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Literature summary extracted from

  • Okabayashi, K.; Nakano, E.
    Purification and properties of mitochondrial malate dehydrogenase from unfertilized eggs of the sea urchin, Anthocidaris crassispina (1984), J. Biochem., 95, 1625-1632.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.37 dithiothreitol
-
 Heliocidaris crassispina
1.1.1.37 Sodium phosphate
-
 Heliocidaris crassispina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 ADP
-
 Heliocidaris crassispina
1.1.1.37 ATP
-
 Heliocidaris crassispina
1.1.1.37 Na2SO3
-
 Heliocidaris crassispina
1.1.1.37 oxaloacetate substrate inhibition Heliocidaris crassispina
1.1.1.37 p-chloromercuriphenylsulfonate
-
 Heliocidaris crassispina
1.1.1.37 sulfite
-
 Heliocidaris crassispina
1.1.1.37 Zn2+
-
 Heliocidaris crassispina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.03
-
 NADH
-
 Heliocidaris crassispina
1.1.1.37 0.6
-
 NAD+
-
 Heliocidaris crassispina
1.1.1.37 7
-
 L-malate
-
 Heliocidaris crassispina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol
-
 Heliocidaris crassispina 5829
-
1.1.1.37 mitochondrion
-
 Heliocidaris crassispina 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.37 CaCl2
-
 Heliocidaris crassispina
1.1.1.37 MgCl2
-
 Heliocidaris crassispina
1.1.1.37 Na2HAsO4
-
 Heliocidaris crassispina
1.1.1.37 NaF
-
 Heliocidaris crassispina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 35000
-
 2 * 35000, SDS-PAGE Heliocidaris crassispina
1.1.1.37 65000
-
-
 Heliocidaris crassispina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Heliocidaris crassispina
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Strongylocentrotus purpuratus
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Heliocidaris crassispina
-
 sea urchin
-
1.1.1.37 Strongylocentrotus purpuratus
-
 sea urchin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Strongylocentrotus purpuratus
1.1.1.37 M-MDH Heliocidaris crassispina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 egg
-
 Heliocidaris crassispina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 1.28
-
-
 Heliocidaris crassispina

Storage Stability

EC Number Storage Stability Organism
1.1.1.37 -20°C, can be stored at least several months without any appreciable loss in activity Heliocidaris crassispina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Heliocidaris crassispina oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Strongylocentrotus purpuratus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Heliocidaris crassispina oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Strongylocentrotus purpuratus oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Heliocidaris crassispina L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Strongylocentrotus purpuratus L-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer 2 * 35000, SDS-PAGE Heliocidaris crassispina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 45
-
 after incubation for 50 min enzyme loses about 90% of its activity Heliocidaris crassispina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 8.2 9.4 reduction of oxaloacetate with NADH Heliocidaris crassispina
1.1.1.37 10.2
-
 malate oxidation with NAD+ Heliocidaris crassispina