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Literature summary extracted from

  • Bradshaw, R.A.; Noyes, B.E.
    L-3-hydroxylacyl coenzyme A dehydrogenase from pig heart muscle (1975), Methods Enzymol., 35, 122-128.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.35 50% saturation with ammonium sulfate solution, 0.1 M potassium phosphate, pH 6.8, 1 mM EDTA, 2 mM beta-mercaptoethanol, 4°C, crystals appear after 2 days Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.35 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Sus scrofa
1.1.1.35 iodoacetamide
-
 Sus scrofa
1.1.1.35 iodoacetic acid
-
 Sus scrofa
1.1.1.35 N-bromsuccinimide
-
 Sus scrofa
1.1.1.35 N-ethylmaleimide
-
 Sus scrofa
1.1.1.35 p-chloromercuribenzoate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 0.06
-
 acetoacetyl-CoA
-
 Sus scrofa
1.1.1.35 0.4
-
 acetoacetyl-pantetheine
-
 Sus scrofa
1.1.1.35 10
-
 acetoacetyl-Nacetylcysteamine
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.35 mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.35 65000
-
 sedimentation equilibrium Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Sus scrofa
-
 acetoacetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 ammonium sulfate, first CM-cellulose, gelfiltration, second and third CM-cellulose Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.35 after denaturation with 2-3 M urea or guanidinium-HCl, 94% activity can be recovered by dialyzing the enzyme at 4°C against 100 mM sodium diphosphate, pH 7.3, 10 mM 2-mercaptoethanol Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.35 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.35 220
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.1.1.35 4°C, 90% ammonium sulfate, at least 6 monts, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Sus scrofa acetoacetyl-CoA + NADH
-
 r
1.1.1.35 acetoacetyl-N-acetylcysteamine + NADH
-
 Sus scrofa (S)-3-hydroxybutyryl-N-acetylcysteamine + NAD+
-
 ?
1.1.1.35 acetoacetyl-pantetheine + NADH
-
 Sus scrofa (S)-3-hydroxybutyryl-pantetheine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.35 dimer
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Sus scrofa