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Literature summary extracted from
- Purification, characterization, and cloning of a eubacterial 3-hydroxy-3-methylglutaryl coenzyme A reductase, a key enzyme involved in biosynthesis of terpenoids (1999), J. Bacteriol., 181, 1256-1263.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.34 | overexpression in Escherichia coli | Streptomyces sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.34 | 0.008 | - |
3-hydroxy-3-methylglutaryl-CoA | - |
Streptomyces sp. |  |
| 1.1.1.34 | 0.062 | - |
NADPH | - |
Streptomyces sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.34 | 41000 | - |
alpha2, 2 * 41000, SDS-PAGE | Streptomyces sp. |
| 1.1.1.34 | 100000 | - |
gel filtration | Streptomyces sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.34 | 3-hydroxy-3-methylglutaryl-CoA + NADPH | Streptomyces sp. | - |
(R)-mevalonate + CoA + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.34 | Streptomyces sp. | - |
CL190 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.34 | - |
Streptomyces sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.34 | 1.6 | - |
- |
Streptomyces sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.34 | 3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Streptomyces sp. | (R)-mevalonate + CoA + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.34 | dimer | alpha2, 2 * 41000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.34 | 35 | 60 | - |
Streptomyces sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.34 | 15 | 60 | - |
Streptomyces sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.34 | 7.2 | - |
- |
Streptomyces sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.34 | NADPH | - |
Streptomyces sp. | |
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Release 2023.1 (January 2023)
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