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Literature summary extracted from

  • Chowdhury, E.K.; Nagata, S.; Misono, H.
    3-Hydroxyisobutyrate dehydrogenase from Pseudomonas putida E23: purification and characterization (1996), Biosci. Biotechnol. Biochem., 60, 2043-2047.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.31 AgNO3
-
 Diutina rugosa
1.1.1.31 AgNO3
-
 Oryctolagus cuniculus
1.1.1.31 AgNO3
-
 Pseudomonas putida
1.1.1.31 HgCl2
-
 Pseudomonas putida
1.1.1.31 p-chloromercuribenzoate
-
 Diutina rugosa
1.1.1.31 p-chloromercuribenzoate
-
 Oryctolagus cuniculus
1.1.1.31 p-chloromercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.31 0.023
-
 NAD+
-
 Oryctolagus cuniculus
1.1.1.31 0.05
-
 NAD+
-
 Diutina rugosa
1.1.1.31 0.061
-
 L-3-hydroxyisobutyrate
-
 Oryctolagus cuniculus
1.1.1.31 0.12
-
 L-3-hydroxyisobutyrate
-
 Pseudomonas putida
1.1.1.31 0.37
-
 L-3-hydroxyisobutyrate
-
 Diutina rugosa
1.1.1.31 0.44
-
 NAD+
-
 Pseudomonas putida
1.1.1.31 1.25
-
 D-3-hydroxyisobutyrate
-
 Diutina rugosa
1.1.1.31 18
-
 3-hydroxypropionate
-
 Pseudomonas putida
1.1.1.31 44
-
 L-serine
-
 Pseudomonas putida
1.1.1.31 83
-
 2-methyl-D,L-serine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.31 30000
-
 alpha4, 4 * 30000, SDS-PAGE Pseudomonas putida
1.1.1.31 74000
-
-
 Oryctolagus cuniculus
1.1.1.31 80000
-
-
 Diutina rugosa
1.1.1.31 120000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.31 L-3-hydroxyisobutyrate + NAD+ Oryctolagus cuniculus
-
 L-2-methyl-3-oxopropionate + NADH
-
 r
1.1.1.31 L-3-hydroxyisobutyrate + NAD+ Pseudomonas putida
-
 L-2-methyl-3-oxopropionate + NADH
-
 ?
1.1.1.31 L-3-hydroxyisobutyrate + NAD+ Pseudomonas putida E23
-
 L-2-methyl-3-oxopropionate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.31 Diutina rugosa
-
-
-
1.1.1.31 Oryctolagus cuniculus
-
-
-
1.1.1.31 Pseudomonas putida
-
 E23
-
1.1.1.31 Pseudomonas putida E23
-
 E23
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.31
-
 Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.31 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.31 36.1
-
-
 Diutina rugosa
1.1.1.31 294
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.31 2-methyl-D,L-serine + NAD+
-
 Pseudomonas putida ?
-
 ?
1.1.1.31 2-methyl-D,L-serine + NAD+
-
 Pseudomonas putida E23 ?
-
 ?
1.1.1.31 3-hydroxypropanoate + NAD+
-
 Oryctolagus cuniculus ?
-
 ?
1.1.1.31 3-hydroxypropanoate + NAD+
-
 Pseudomonas putida ?
-
 ?
1.1.1.31 3-hydroxypropanoate + NAD+
-
 Pseudomonas putida E23 ?
-
 ?
1.1.1.31 D-3-hydroxyisobutyrate + NAD+
-
 Diutina rugosa methylmalonate semialdehyde + NADH + H+
-
 r
1.1.1.31 L-3-hydroxyisobutyrate + NAD+
-
 Oryctolagus cuniculus L-2-methyl-3-oxopropionate + NADH
-
 r
1.1.1.31 L-3-hydroxyisobutyrate + NAD+
-
 Pseudomonas putida L-2-methyl-3-oxopropionate + NADH
-
 ?
1.1.1.31 L-3-hydroxyisobutyrate + NAD+
-
 Pseudomonas putida E23 L-2-methyl-3-oxopropionate + NADH
-
 ?
1.1.1.31 L-3-hydroxyisobutyrate + NAD+
-
 Diutina rugosa methylmalonate semialdehyde + NADH + H+
-
 r
1.1.1.31 L-serine + NAD+
-
 Pseudomonas putida ?
-
 ?
1.1.1.31 L-serine + NAD+
-
 Pseudomonas putida E23 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.31 homodimer
-
 Oryctolagus cuniculus
1.1.1.31 homodimer
-
 Diutina rugosa
1.1.1.31 tetramer alpha4, 4 * 30000, SDS-PAGE Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.31 9 10.5
-
 Diutina rugosa
1.1.1.31 9 11
-
 Oryctolagus cuniculus
1.1.1.31 10
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.31 4.5 7.3
-
 Diutina rugosa
1.1.1.31 5.7 9.3
-
 Pseudomonas putida
1.1.1.31 7 11.5
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.31 NAD+
-
 Oryctolagus cuniculus
1.1.1.31 NAD+
-
 Pseudomonas putida
1.1.1.31 NAD+
-
 Diutina rugosa