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Literature summary extracted from

  • Nakada T.; Fukui, T.; Saito, T.; Miki, K.; Oji, C.; Matsuda, S.; Ushijima, A.; Tomita, K.
    Purification and properties of D-beta-hydroxybutyrate dehydrogenase from Zoogloea ramiger I-16-M (1981), J. Biochem., 89, 625-635.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 2-mercaptoethanol 10 mM, 41% increase Zoogloea ramigera I-16-M
1.1.1.30 dithiothreitol 10 mM, 31% increase Zoogloea ramigera I-16-M
1.1.1.30 EDTA 0.5 mM, 21% increase Zoogloea ramigera I-16-M
1.1.1.30 KCN 0.1 mM, 37% increase Zoogloea ramigera I-16-M
1.1.1.30 phosphatidylcholine
-
 Zoogloea ramigera I-16-M
1.1.1.30 reduced glutathione 10 mM, 38% increase Zoogloea ramigera I-16-M

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.30 2-Hydroxybutyrate 10 mM, 57% inhibition Zoogloea ramigera I-16-M
1.1.1.30 2-oxobutyrate 10 mM, 29% inhibition Zoogloea ramigera I-16-M
1.1.1.30 5,5'-dithiobis(2-nitrobenzoic acid) 0.15 mM, 63% inhibition Zoogloea ramigera I-16-M
1.1.1.30 acetoacetate inhibition above 1 mM, 2 mM, 89% inhibition Zoogloea ramigera I-16-M
1.1.1.30 acetoacetyl-CoA 1 mM, 29% inhibition Zoogloea ramigera I-16-M
1.1.1.30 acetyl-CoA 1 mM, 82% inhibition Zoogloea ramigera I-16-M
1.1.1.30 Butyrate 10 mM, 20% inhibition Zoogloea ramigera I-16-M
1.1.1.30 crotonate 10 mM, 20% inhibition Zoogloea ramigera I-16-M
1.1.1.30 CuSO4 1 mM, 81% inhibition Zoogloea ramigera I-16-M
1.1.1.30 HgCl2 0.1 mM, 100% inhibition Zoogloea ramigera I-16-M
1.1.1.30 L-3-hydroxybutyrate 5 mM, 73% inhibition Zoogloea ramigera I-16-M
1.1.1.30 malonate 10 mM, 66% inhibition, oxidation Zoogloea ramigera I-16-M
1.1.1.30 MnCl2 1 mM, 50% inhibition Zoogloea ramigera I-16-M
1.1.1.30 NADH 0.15 mM, 48% inhibition Zoogloea ramigera I-16-M
1.1.1.30 p-chloromercuribenzoate 0.1 mM, 100% inhibition Zoogloea ramigera I-16-M
1.1.1.30 pyruvate 4 mM, 5% inhibition Zoogloea ramigera I-16-M
1.1.1.30 succinate 10 mM, 12% inhibition Zoogloea ramigera I-16-M

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.015
-
 NADH
-
 Zoogloea ramigera I-16-M
1.1.1.30 0.057
-
 NAD+
-
 Zoogloea ramigera I-16-M
1.1.1.30 0.15
-
 acetoacetate
-
 Zoogloea ramigera I-16-M
1.1.1.30 0.32
-
 (R)-3-hydroxybutanoate
-
 Zoogloea ramigera I-16-M

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.30 Ca2+ 10 mM 20% increase in activity Zoogloea ramigera I-16-M
1.1.1.30 Mg2+ 10 mM 41% increase in activity Zoogloea ramigera I-16-M

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 28000
-
 alpha4, 4 * 28000, SDS-PAGE Zoogloea ramigera I-16-M
1.1.1.30 112000
-
 gel filtration Zoogloea ramigera I-16-M

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Zoogloea ramigera I-16-M
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Zoogloea ramigera I-16-M
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 ammonium sulfate, DEAE-Sepharose, 60°C, 10 min, hydroxylapatite, octyl-Sepharose, Sephadex G-200, DEAE-Sepharose Zoogloea ramigera I-16-M

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 123
-
-
 Zoogloea ramigera I-16-M

Storage Stability

EC Number Storage Stability Organism
1.1.1.30 -20°C, 1 mg enzyme/ml, 0.2 mM potassium phosphate pH 8.0, 10% glycerol, 10 mM 2-mercaptoethanol, 6 months, 0% inactivation, 0°C, 0.1 mg enzyme/ml, 1 day, 50% inactivation Zoogloea ramigera I-16-M

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Zoogloea ramigera I-16-M acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Zoogloea ramigera I-16-M acetoacetate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 tetramer alpha4, 4 * 28000, SDS-PAGE Zoogloea ramigera I-16-M

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.30 8
-
 oxidation of beta-hydroxybutyrate Zoogloea ramigera I-16-M

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.30 5.5 7.5 acetoacetate reduction Zoogloea ramigera I-16-M

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Zoogloea ramigera I-16-M