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Literature summary extracted from

  • Hagishita, T.; Yoshida, T.; Izumi, Y.; Mitsunaga, T.
    Immunological characterization of serine-glyoxylate aminotransferase and hydroxypyruvate reductase from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2 (1996), FEMS Microbiol. Lett., 142, 49-52.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.29 38000
-
 2 * 38000, SDS-PAGE Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.29 hydroxypyruvate + NADH Hyphomicrobium methylovorum
-
 D-glycerate + NAD+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Spinacia oleracea
-
 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Cucumis sativus
-
 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Methylobacterium organophilum
-
 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Hyphomicrobium methylovorum GM2
-
 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Methylorubrum extorquens AM1
-
 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Methylobacterium organophilum XX
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.29 Hyphomicrobium methylovorum
-
-
-
1.1.1.81 Cucumis sativus
-
-
-
1.1.1.81 Hyphomicrobium methylovorum GM2
-
-
-
1.1.1.81 Methylobacterium organophilum
-
-
-
1.1.1.81 Methylorubrum extorquens AM1
-
-
-
1.1.1.81 Spinacia oleracea
-
-
-
2.6.1.45 Hyphomicrobium methylovorum
-
-
-
2.6.1.45 Hyphomicrobium sp.
-
 strains NCIB10099, X, JTS-811, ZV622 and 53-49
-
2.6.1.45 Methylobacterium organophilum
-
-
-
2.6.1.45 Methylobacterium organophilum XX
-
-
-
2.6.1.45 Methylorubrum extorquens
-
-
-
2.6.1.45 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.29
-
 Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.29 additional information
-
 activities of several Hypomicrobium and Methylobacterium strains listed Hyphomicrobium methylovorum
1.1.1.81 0.1
-
 in cell-free extracts Cucumis sativus
1.1.1.81 0.9
-
 in cell-free extracts Spinacia oleracea
1.1.1.81 1.5
-
 in cell-free extracts Methylobacterium organophilum
1.1.1.81 1.7
-
 in cell-free extracts Methylorubrum extorquens AM1
1.1.1.81 1.9
-
 in cell-free extracts Hyphomicrobium methylovorum GM2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.29 hydroxypyruvate + NADH
-
 Hyphomicrobium methylovorum D-glycerate + NAD+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Spinacia oleracea D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Cucumis sativus D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Methylobacterium organophilum D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Hyphomicrobium methylovorum GM2 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Methylorubrum extorquens AM1 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Methylobacterium organophilum XX D-glycerate + NAD(P)+ + H+
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Hyphomicrobium sp. 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Hyphomicrobium methylovorum 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Methylorubrum extorquens 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Methylobacterium organophilum 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Methylobacterium organophilum XX 3-hydroxypyruvate + glycine
-
 ?
2.6.1.45 L-serine + glyoxylate
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 3-hydroxypyruvate + glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.29 dimer 2 * 38000, SDS-PAGE Hyphomicrobium methylovorum