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Literature summary extracted from

  • Kleczkowski, L.A.; Randall, D.D.; Edwards, G.E.
    Oxalate as a potent and selective inhibitor of spinach (Spinacia oleracea) leaf NADPH-dependent hydroxypyruvate reductase (1991), Biochem. J., 276, 125-127.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.81 oxalate 2 mM, 90% inhibition Spinacia oleracea
1.1.1.81 phosphohydroxypyruvate 2 mM, 44-71% inhibition Spinacia oleracea
1.1.1.81 Tartronate 2 mM, 30-55% inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.29 0.09
-
 Hydroxypyruvate
-
 Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.29 hydroxypyruvate + NADH Zea mays
-
 D-glycerate + NAD+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H Spinacia oleracea
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.29 Hordeum vulgare
-
 barley
-
1.1.1.29 Zea mays
-
-
-
1.1.1.81 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.29 leaf
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.81 260
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.29 hydroxypyruvate + NADH
-
 Hordeum vulgare D-glycerate + NAD+
-
 r
1.1.1.29 hydroxypyruvate + NADH
-
 Zea mays D-glycerate + NAD+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Spinacia oleracea D-glycerate + NAD(P)+ + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.29 NADH
-
 Zea mays