Literature summary extracted from

Kochhar, S.; Lamzin, V.S.; Razeto, A.; Delley, M.; Hottinger, H.; Germond, J.E.
Roles of His205, His296, His303 and Asp259 in catalysis by NAD+-specific D-lactate dehydrogenase (2000), Eur. J. Biochem., 267, 1633-1639.

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.1.1.28	D259N	56fold reduction in kcat, 5fold lowering of Km, shifting of the enzymatic activity profile towards the acidic range by two units	Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28	H205K	125-fold reduction in Kcat	Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28	H296K	no significant change	Lactobacillus delbrueckii subsp. bulgaricus
1.1.1.28	H303K	Km for pyruvate increased fourfold	Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.28	(R)-lactate + NAD+	Lactobacillus delbrueckii subsp. bulgaricus	-	pyruvate + NADH	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.28	Lactobacillus delbrueckii subsp. bulgaricus	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.28	(R)-lactate + NAD+	-	Lactobacillus delbrueckii subsp. bulgaricus	pyruvate + NADH	-	?

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Release 2023.1 (January 2023)