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Literature summary extracted from

  • Stoll, V.S.; Manohar, A.V.; Gillon, W.; MacFarlane, E.L.; Hynes, R.C.; Pai, E.F.
    A thioredoxin fusion protein of VanH, a D-lactate dehydrogenase from Enterococcus faecium: cloning, expression, purification, kinetic analysis, and crystallization (1998), Protein Sci., 7, 1147-1155.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.28 2-mercaptoethanol strongly reducing enviroment required Enterococcus faecium
1.1.1.28 acetate
-
Enterococcus faecium
1.1.1.28 phosphate
-
Enterococcus faecium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 expression in Escherichia coli as thioredoxin fusion protein Enterococcus faecium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.28 hexagonal and tetragonal crystal forms, tetragonal form diffracted to 3.0 A resolution Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.0035
-
NADPH
-
Enterococcus faecium
1.1.1.28 0.019
-
NADH
-
Enterococcus faecium
1.1.1.28 1.5
-
pyruvate
-
Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Enterococcus faecium
-
pyruvate + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Enterococcus faecium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
Enterococcus faecium pyruvate + NADH
-
?
1.1.1.28 pyruvate + NADPH + H+
-
Enterococcus faecium (R)-lactate + NADP+
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
Enterococcus faecium
1.1.1.28 NADH
-
Enterococcus faecium