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Literature summary extracted from
- Purification, properties and DNA sequence of the D-lactate dehydrogenase from Leuconostoc mesenteroides subsp. cremoris (1995), Res. Microbiol., 146, 291-302.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.28 | expression in Escherichia coli | Leuconostoc mesenteroides subsp. cremoris |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.28 | 0.03 | - |
NADH | - |
Leuconostoc mesenteroides subsp. cremoris |  |
| 1.1.1.28 | 0.16 | - |
NAD+ | - |
Leuconostoc mesenteroides subsp. cremoris | |
| 1.1.1.28 | 0.3 | - |
pyruvate | - |
Leuconostoc mesenteroides subsp. cremoris | |
| 1.1.1.28 | 19 | - |
(R)-lactate | - |
Leuconostoc mesenteroides subsp. cremoris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.28 | 40000 | - |
2 * 40000, SDS-PAGE | Leuconostoc mesenteroides subsp. cremoris |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.28 | (R)-lactate + NAD+ | Leuconostoc mesenteroides subsp. cremoris | - |
pyruvate + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.28 | Leuconostoc mesenteroides subsp. cremoris | P51011 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.28 | - |
Leuconostoc mesenteroides subsp. cremoris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.28 | (R)-lactate + NAD+ | - |
Leuconostoc mesenteroides subsp. cremoris | pyruvate + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.28 | dimer | 2 * 40000, SDS-PAGE | Leuconostoc mesenteroides subsp. cremoris |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.28 | NAD+ | - |
Leuconostoc mesenteroides subsp. cremoris | |
| 1.1.1.28 | NADH | - |
Leuconostoc mesenteroides subsp. cremoris | |
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