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Literature summary extracted from

  • Marchat, L.; Loiseau, P.M.; Petek, F.
    Purification and characterization of lactate dehydrogenase isoenzymes 1 and 2 from Molinema dessetae (Nematoda:Filarioidea) (1996), Parasitol. Res., 82, 672-680.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 Hg2+
-
 Molinema dessetae
1.1.1.27 iodoacetate
-
 Molinema dessetae
1.1.1.27 oxalate noncompetitive with pyruvate, competitive with lactate Molinema dessetae
1.1.1.27 oxamate competitive with pyruvate, noncompetitive with lactate Molinema dessetae
1.1.1.27 p-chloromercuribenzoate
-
 Molinema dessetae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.18
-
 NAD+
-
 Molinema dessetae
1.1.1.27 0.25
-
 NADH
-
 Molinema dessetae
1.1.1.27 0.34
-
 pyruvate
-
 Molinema dessetae
1.1.1.27 2.5
-
 (S)-lactate
-
 Molinema dessetae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 58000
-
 2 * 58000, isoenzyme LDH1 and LDH2, SDS-PAGE Molinema dessetae
1.1.1.27 113000
-
 gel filtration Molinema dessetae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Molinema dessetae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 two major isoenzymes LDH1 and LDH2 Molinema dessetae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 80.27
-
-
 Molinema dessetae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ pyruvate reduction is the favored reaction Molinema dessetae pyruvate + NADH + H+
-
 r
1.1.1.27 pyruvate + NADH + H+ pyruvate reduction is the favored reaction Molinema dessetae (S)-lactate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 dimer 2 * 58000, isoenzyme LDH1 and LDH2, SDS-PAGE Molinema dessetae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 50
-
 15 min, 2% loss of activity Molinema dessetae
1.1.1.27 55
-
 stable below Molinema dessetae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 6.8
-
 reduction of pyruvate Molinema dessetae
1.1.1.27 8.6
-
 oxidation of lactate Molinema dessetae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.27 6 8 pH 6.0: 85% of maximal activity, pH 8.0: 45% of maximal activity, reduction of pyruvate Molinema dessetae
1.1.1.27 8 9.6 pH 8.0: 60% of maximal activity, pH 9.6: 45% of maximal activity, oxidation of lactate Molinema dessetae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+ coenzyme Molinema dessetae
1.1.1.27 NADH coenzyme Molinema dessetae