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Literature summary extracted from
- Purification and properties of L-lactate dehydrogenase from Acinetobacter calcoaceticus (1986), FEMS Microbiol. Lett., 36, 183-186.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.27 | Hg2+ | HgCl2 | Acinetobacter calcoaceticus |  |
| 1.1.1.27 | oxalate | - |
Acinetobacter calcoaceticus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.27 | 0.083 | - |
(S)-lactate | - |
Acinetobacter calcoaceticus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.27 | integral to plasma membrane | - |
Acinetobacter calcoaceticus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.27 | 40000 | - |
x * 40000, oligomer, SDS-PAGE | Acinetobacter calcoaceticus |
| 1.1.1.27 | 670000 | - |
above, gel filtration | Acinetobacter calcoaceticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.27 | Acinetobacter calcoaceticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.27 | - |
Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.27 | 27.6 | - |
- |
Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.27 | (S)-lactate + NAD+ | - |
Acinetobacter calcoaceticus | pyruvate + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.27 | ? | x * 40000, oligomer, SDS-PAGE | Acinetobacter calcoaceticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.27 | 7.5 | - |
- |
Acinetobacter calcoaceticus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.27 | FMN | contains non-covalently bound FMN as prosthetic group | Acinetobacter calcoaceticus | |
| 1.1.1.27 | NAD+ | coenzyme | Acinetobacter calcoaceticus | |
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