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Literature summary extracted from
- The enzymic reduction of glyoxylate and hydroxypyruvate in leaves of higher plants (1992), Plant Physiol., 100, 552-556.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.26 | additional information | mutant lacking cytosolic hydroxypyruvate-metabolizing activity (HPR-1) | Hordeum vulgare |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.26 | Acetohydroxamate | inhibits only the cytosolic enzyme type with preference for glyoxylate as substrate | Pisum sativum |  |
| 1.1.1.26 | Acetohydroxamate | inhibits only the cytosolic enzyme type with preference for glyoxylate as substrate | Spinacia oleracea | |
| 1.1.1.26 | oxalate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Pisum sativum | |
| 1.1.1.26 | oxalate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Spinacia oleracea | |
| 1.1.1.26 | Tartronate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Pisum sativum | |
| 1.1.1.26 | Tartronate | inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate | Spinacia oleracea | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.26 | 0.07 | 0.11 | glyoxylate | NADPH, cytosolic enzyme | Spinacia oleracea | |
| 1.1.1.26 | 0.07 | 0.12 | Hydroxypyruvate | NADH, peroxisomal enzyme type with preference for hydroxypyruvate and NADH as substrates | Spinacia oleracea | |
| 1.1.1.26 | 0.8 | - |
Hydroxypyruvate | NADPH, cytosolic enzyme type with preference for hydroxypyruvate and NADPH as substrates | Spinacia oleracea | |
| 1.1.1.26 | 1.1 | - |
glyoxylate | NADPH, cytosolic enzyme type with preference for hydroxypyruvate as substrate | Spinacia oleracea | |
| 1.1.1.26 | 10 | 25 | glyoxylate | NADH, peroxisomal enzyme type with preference for hydroxypyruvate and NADH as substrates | Spinacia oleracea | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.26 | chloroplast | utilizes NADH and NADPH equally | Spinacia oleracea | 9507 | - |
| 1.1.1.26 | chloroplast | utilizes NADH and NADPH equally | Pisum sativum | 9507 | - |
| 1.1.1.26 | cytosol | - |
Hordeum vulgare | 5829 | - |
| 1.1.1.26 | cytosol | NADP+/NADPH preferring type | Spinacia oleracea | 5829 | - |
| 1.1.1.26 | cytosol | NADP+/NADPH preferring type | Pisum sativum | 5829 | - |
| 1.1.1.26 | peroxisome | - |
Hordeum vulgare | 5777 | - |
| 1.1.1.26 | peroxisome | NAD+/NADH preferring type | Spinacia oleracea | 5777 | - |
| 1.1.1.26 | peroxisome | NAD+/NADH preferring type | Pisum sativum | 5777 | - |
| 1.1.1.26 | peroxisome | NAD+/NADH preferring type | Petroselinum crispum | 5777 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.26 | 33000 | - |
4 * 33000, cytosolic enzyme | Spinacia oleracea |
| 1.1.1.26 | 38000 | - |
2 * 38000, cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
| 1.1.1.26 | 70000 | - |
cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
| 1.1.1.26 | 90000 | - |
peroxisomal enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
| 1.1.1.26 | 125000 | - |
cytosolic enzyme | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.26 | glycolate + NAD+ | Spinacia oleracea | - |
glyoxylate + NADH | - |
r | |
| 1.1.1.26 | glycolate + NAD+ | Pisum sativum | - |
glyoxylate + NADH | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | Spinacia oleracea | - |
glycolate + NAD+ | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | Pisum sativum | - |
glycolate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | Pisum sativum | - |
D-glycerate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | Spinacia oleracea | located exclusively in the peroxisomes, prefers NAD+/NADH | D-glycerate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | Petroselinum crispum | located exclusively in the peroxisomes, prefers NAD+/NADH | D-glycerate + NAD+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.26 | Hordeum vulgare | - |
barley | - |
| 1.1.1.26 | Nicotiana tabacum | - |
tobacco | - |
| 1.1.1.26 | Petroselinum crispum | - |
parsley | - |
| 1.1.1.26 | Pisum sativum | - |
- |
- |
| 1.1.1.26 | Spinacia oleracea | - |
spinach | - |
| 1.1.1.26 | Zea mays | - |
maize | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.26 | - |
Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.26 | leaf | - |
Spinacia oleracea | - |
| 1.1.1.26 | leaf | - |
Pisum sativum | - |
| 1.1.1.26 | leaf | - |
Zea mays | - |
| 1.1.1.26 | leaf | - |
Nicotiana tabacum | - |
| 1.1.1.26 | leaf | mutant without cytosolic hydroxypyruvate-metabolizing activity | Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.26 | 0.05 | 0.23 | NADPH, cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
| 1.1.1.26 | 0.3 | 1.5 | NADH, peroxisomal enzyme type with preference for hydroxypyruvate and NADH as substrates | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.26 | glycolate + NAD+ | - |
Spinacia oleracea | glyoxylate + NADH | - |
r | |
| 1.1.1.26 | glycolate + NAD+ | - |
Pisum sativum | glyoxylate + NADH | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | - |
Spinacia oleracea | glycolate + NAD+ | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | - |
Pisum sativum | glycolate + NAD+ | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | equilibrium is very far in direction of glycolate formation | Spinacia oleracea | glycolate + NAD+ | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | equilibrium is very far in direction of glycolate formation | Pisum sativum | glycolate + NAD+ | - |
r | |
| 1.1.1.26 | glyoxylate + NADH | equilibrium is very far in direction of glycolate formation | Nicotiana tabacum | glycolate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | - |
Spinacia oleracea | D-glycerate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | - |
Pisum sativum | D-glycerate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | - |
Petroselinum crispum | D-glycerate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | located exclusively in the peroxisomes, prefers NAD+/NADH | Spinacia oleracea | D-glycerate + NAD+ | - |
r | |
| 1.1.1.26 | hydroxypyruvate + NADH | located exclusively in the peroxisomes, prefers NAD+/NADH | Petroselinum crispum | D-glycerate + NAD+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.26 | dimer | 2 * 38000, cytosolic enzyme with preference for hydroxypyruvate as substrate | Spinacia oleracea |
| 1.1.1.26 | tetramer | 4 * 33000, cytosolic enzyme | Spinacia oleracea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.26 | 5.8 | 7.4 | the optimal pH for hydroxypyruvate reduction is more acidic than that for glyoxylate reduction | Spinacia oleracea |
| 1.1.1.26 | 5.8 | 7.4 | the optimal pH for hydroxypyruvate reduction is more acidic than that for glyoxylate reduction | Pisum sativum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.26 | NAD+ | - |
Pisum sativum | |
| 1.1.1.26 | NAD+ | - |
Nicotiana tabacum | |
| 1.1.1.26 | NAD+ | enzyme of peroxisomes and chloroplasts prefer NAD+, enzymes of the cytosol prefer NADP+ | Spinacia oleracea | |
| 1.1.1.26 | NADH | - |
Pisum sativum | |
| 1.1.1.26 | NADH | - |
Nicotiana tabacum | |
| 1.1.1.26 | NADH | enzyme of peroxisomes and chloroplasts prefer NADH, enzymes of the cytosol prefer NADPH | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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