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Literature summary extracted from

  • Lourenco, E.J.; Neves, V.A.
    Partial purification and some properties of shikimate dehydrogenase from tomatoes (1984), Phytochemistry, 23, 497-499.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.25 Borate
-
Solanum lycopersicum
1.1.1.25 Cu2+
-
Solanum lycopersicum
1.1.1.25 Hg2+
-
Solanum lycopersicum
1.1.1.25 N-ethylmaleimide
-
Solanum lycopersicum
1.1.1.25 NH4+
-
Solanum lycopersicum
1.1.1.25 p-chloromercuribenzoate
-
Solanum lycopersicum
1.1.1.25 protocatechuic acid
-
Solanum lycopersicum
1.1.1.25 Zn2+
-
Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.01
-
NADP+
-
Solanum lycopersicum
1.1.1.25 0.038
-
shikimate
-
Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.25 additional information no requirement for divalent ions Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 73000
-
gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 shikimate + NADP+ Solanum lycopersicum
-
3-dehydroshikimate + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Solanum lycopersicum
-
tomato
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.25 fruit
-
Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.25 0.28
-
-
Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 shikimate + NADP+
-
Solanum lycopersicum 3-dehydroshikimate + NADPH + H+
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.25 50
-
5 min, 85% loss of activity Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 9.1
-
-
Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.25 6 11 no activity below and above Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 additional information no activity with NAD+ Solanum lycopersicum
1.1.1.25 NADP+ specific for Solanum lycopersicum
1.1.1.25 NADPH specific for Solanum lycopersicum