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Literature summary extracted from

  • Tae-Kang, K.; Choi, J.H.; Rhee, I.K.
    Purification and characterization of a cyclohexanol dehydrogenase from Rhodococcus sp. TK6 (2002), J. Microbiol. Biotechnol., 12, 39-45.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.245 Al3+ almost complete inhibition of activity at 10 mM Rhodococcus sp.
1.1.1.245 Ca2+ inhibition of activity at 10 mM Rhodococcus sp.
1.1.1.245 Co2+ inhibition of activity at 10 mM Rhodococcus sp.
1.1.1.245 Cu2+ almost complete inhibition of activity at 10 mM Rhodococcus sp.
1.1.1.245 Mn2+ 93% inhibition of activity at 10 mM Rhodococcus sp.
1.1.1.245 Zn2+ 91% inhibition of activity at 10 mM Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.245 0.0017
-
 Cyclohexanol isoenzyme II at pH 7.6 Rhodococcus sp.
1.1.1.245 0.0028
-
 trans-Cyclohexane-1,2-diol isoenzyme II at pH 7.6 Rhodococcus sp.
1.1.1.245 0.0135
-
 hexane-1,2-diol isoenzyme II at pH 7.6 Rhodococcus sp.
1.1.1.245 0.0137
-
 trans-cyclopentane-1,2-diol isoenzyme II at pH 7.6 Rhodococcus sp.
1.1.1.245 0.0142
-
 Cyclopentanol isoenzyme II at pH 7.6 Rhodococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.245 Ba2+ increase of activity at 10 mM Rhodococcus sp.
1.1.1.245 Mg2+ increase of activity at 10 mM Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.245 28000
-
 2 * 28000, SDS-PAGE, isoenzyme II Rhodococcus sp.
1.1.1.245 60000
-
 gel filtration, isoenzyme II Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.245 Rhodococcus sp.
-
-
-
1.1.1.245 Rhodococcus sp. TK6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.245 9.8fold, isoenzyme II Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.245 127
-
 purified enzyme Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.245 1-butanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. butanal + NADH
-
 ?
1.1.1.245 1-hexanol + NAD+ relative oxidation activity at 21% the rate of cyclohexanol oxidation Rhodococcus sp. hexanal + NADH
-
 ?
1.1.1.245 1-pentanol + NAD+ relative activity at 23% the rate of cyclohexanol oxidation Rhodococcus sp. n-pentanal + NADH
-
 ?
1.1.1.245 1-propanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. propionaldehyde + NADH
-
 ?
1.1.1.245 2-butanol + NAD+ relative activity at 44% the rate of cyclohexanol oxidation Rhodococcus sp. 2-butanone + NADH
-
 ?
1.1.1.245 2-methyl-1-butanol + NAD+ relative activity at 26% the rate of cyclohexanol oxidation Rhodococcus sp. 2-methyl-1-butanal + NADH
-
 ?
1.1.1.245 2-methyl-1-propanol + NAD+ relative activity at 17% the rate of cyclohexanol oxidation Rhodococcus sp. isobutyraldehyde + NADH
-
 ?
1.1.1.245 2-methyl-2-propanol + NAD+ relative activity at 16% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
 ?
1.1.1.245 2-propanol + NAD+ relative activity at 17% the rate of cyclohexanol oxidation Rhodococcus sp. acetone + NADH
-
 ?
1.1.1.245 cis-cyclopentane-1,2-diol + NAD+ relative activity at 27% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
 ?
1.1.1.245 cyclohexan-1,2-diol + NAD+ relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,2-dione + NADH
-
 ?
1.1.1.245 cyclohexan-1,2-diol + NAD+ relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,2-dione + NADH
-
 ?
1.1.1.245 cyclohexan-1,2-diol + NAD+ relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,2-dione + NADH
-
 ?
1.1.1.245 cyclohexan-1,2-diol + NAD+ relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 cyclohexane-1,2-dione + NADH
-
 ?
1.1.1.245 cyclohexane-1,3-diol + NAD+ relative oxidation activity at 28% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,3-dione + NADH
-
 ?
1.1.1.245 cyclohexane-1,4-diol + NAD+ relative oxidation activity at 22% the rate of cyclohexanol oxidation Rhodococcus sp. cyclohexane-1,4-dione + NADH
-
 ?
1.1.1.245 cyclohexanol + NAD+
-
 Rhodococcus sp. cyclohexanone + NADH
-
 ?
1.1.1.245 cyclohexanol + NAD+
-
 Rhodococcus sp. TK6 cyclohexanone + NADH
-
 ?
1.1.1.245 cyclopentanol + NAD+ relative oxidation activity at 77% the rate of cyclohexanol oxidation Rhodococcus sp. cyclopentanone + NADH
-
 ?
1.1.1.245 cyclopentanol + NAD+ relative oxidation activity at 77% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 cyclopentanone + NADH
-
 ?
1.1.1.245 ethane-1,2-diol + NAD+ relative activity at 5% the rate of cyclohexanol oxidation Rhodococcus sp. glyoxal + NADH
-
 ?
1.1.1.245 ethanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. acetaldehyde + NADH
-
 ?
1.1.1.245 ethanol + NAD+ relative activity at 20% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 acetaldehyde + NADH
-
 ?
1.1.1.245 hexane-1,2-diol + NAD+ relative activity at 84% the rate of cyclohexanol oxidation Rhodococcus sp. 2-oxohexanal + NADH
-
 ?
1.1.1.245 hexane-1,6-diol + NAD+ relative activity at 21% the rate of cyclohexanol oxidation Rhodococcus sp. hexanedial + NADH
-
 ?
1.1.1.245 methanol + NAD+ relative activity at 2% the rate of cyclohexanol oxidation Rhodococcus sp. formaldehyde + NADH + H+
-
 ?
1.1.1.245 methanol + NAD+ relative activity at 2% the rate of cyclohexanol oxidation Rhodococcus sp. TK6 formaldehyde + NADH + H+
-
 ?
1.1.1.245 propane-1,2-diol + NAD+ relative activity at 30% the rate of cyclohexanol oxidation Rhodococcus sp. methylglyoxal + NADH
-
 ?
1.1.1.245 propane-1,3-diol + NAD+ relative activity at 7% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
 ?
1.1.1.245 trans-cyclohexane-1,2-diol + NAD+
-
 Rhodococcus sp. cyclohexane-1,2-dione + NADH + H+
-
 ?
1.1.1.245 trans-cyclopentane-1,2-diol + NAD+ relative activity at 114% the rate of cyclohexanol oxidation Rhodococcus sp. ? + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.245 dimer 2 * 28000, SDS-PAGE, isoenzyme II Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.245 cyclohexanol dehydrogenase II isoenzyme Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.245 4
-
 labile Rhodococcus sp.
1.1.1.245 40
-
 90% activity after incubation for 20 min Rhodococcus sp.
1.1.1.245 50
-
 less than 20% activity after incubation for 20 min Rhodococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.245 7.5 8.5 stable Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.245 NAD+ isoenzyme II Rhodococcus sp.