BRENDA - Enzyme Database show

Purification and characterization of a cyclohexanol dehydrogenase from Rhodococcus sp. TK6

Tae-Kang, K.; Choi, J.H.; Rhee, I.K.; J. Microbiol. Biotechnol. 12, 39-45 (2002)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.245
Al3+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Ca2+
inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Co2+
inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Cu2+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Mn2+
93% inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Zn2+
91% inhibition of activity at 10 mM
Rhodococcus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.245
0.0017
-
Cyclohexanol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0028
-
trans-Cyclohexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0135
-
hexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0137
-
trans-cyclopentane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0142
-
Cyclopentanol
isoenzyme II at pH 7.6
Rhodococcus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.245
Ba2+
increase of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Mg2+
increase of activity at 10 mM
Rhodococcus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.245
28000
-
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
1.1.1.245
60000
-
gel filtration, isoenzyme II
Rhodococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.245
Rhodococcus sp.
-
-
-
1.1.1.245
Rhodococcus sp. TK6
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.245
9.8fold, isoenzyme II
Rhodococcus sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.245
127
-
purified enzyme
Rhodococcus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.245
1-butanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
butanal + NADH
-
-
-
?
1.1.1.245
1-hexanol + NAD+
relative oxidation activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanal + NADH
-
-
-
?
1.1.1.245
1-pentanol + NAD+
relative activity at 23% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
n-pentanal + NADH
-
-
-
?
1.1.1.245
1-propanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
propionaldehyde + NADH
-
-
-
?
1.1.1.245
2-butanol + NAD+
relative activity at 44% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-butanone + NADH
-
-
-
?
1.1.1.245
2-methyl-1-butanol + NAD+
relative activity at 26% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-methyl-1-butanal + NADH
-
-
-
?
1.1.1.245
2-methyl-1-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
isobutyraldehyde + NADH
-
-
-
?
1.1.1.245
2-methyl-2-propanol + NAD+
relative activity at 16% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
1.1.1.245
2-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetone + NADH
-
-
-
?
1.1.1.245
cis-cyclopentane-1,2-diol + NAD+
relative activity at 27% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,3-dione + NADH
-
-
-
?
1.1.1.245
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 22% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,4-dione + NADH
-
-
-
?
1.1.1.245
cyclohexanol + NAD+
-
286383
Rhodococcus sp.
cyclohexanone + NADH
-
-
-
?
1.1.1.245
cyclohexanol + NAD+
-
286383
Rhodococcus sp. TK6
cyclohexanone + NADH
-
-
-
?
1.1.1.245
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclopentanone + NADH
-
-
-
?
1.1.1.245
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclopentanone + NADH
-
-
-
?
1.1.1.245
ethane-1,2-diol + NAD+
relative activity at 5% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
glyoxal + NADH
-
-
-
?
1.1.1.245
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetaldehyde + NADH
-
-
-
?
1.1.1.245
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
acetaldehyde + NADH
-
-
-
?
1.1.1.245
hexane-1,2-diol + NAD+
relative activity at 84% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-oxohexanal + NADH
-
-
-
?
1.1.1.245
hexane-1,6-diol + NAD+
relative activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanedial + NADH
-
-
-
?
1.1.1.245
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
formaldehyde + NADH + H+
-
-
-
?
1.1.1.245
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
formaldehyde + NADH + H+
-
-
-
?
1.1.1.245
propane-1,2-diol + NAD+
relative activity at 30% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
methylglyoxal + NADH
-
-
-
?
1.1.1.245
propane-1,3-diol + NAD+
relative activity at 7% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
1.1.1.245
trans-cyclopentane-1,2-diol + NAD+
relative activity at 114% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.245
dimer
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.245
4
-
labile
Rhodococcus sp.
1.1.1.245
40
-
90% activity after incubation for 20 min
Rhodococcus sp.
1.1.1.245
50
-
less than 20% activity after incubation for 20 min
Rhodococcus sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.245
7.5
8.5
stable
Rhodococcus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.245
NAD+
isoenzyme II
Rhodococcus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.245
NAD+
isoenzyme II
Rhodococcus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.245
Al3+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Ca2+
inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Co2+
inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Cu2+
almost complete inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Mn2+
93% inhibition of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Zn2+
91% inhibition of activity at 10 mM
Rhodococcus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.245
0.0017
-
Cyclohexanol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0028
-
trans-Cyclohexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0135
-
hexane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0137
-
trans-cyclopentane-1,2-diol
isoenzyme II at pH 7.6
Rhodococcus sp.
1.1.1.245
0.0142
-
Cyclopentanol
isoenzyme II at pH 7.6
Rhodococcus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.245
Ba2+
increase of activity at 10 mM
Rhodococcus sp.
1.1.1.245
Mg2+
increase of activity at 10 mM
Rhodococcus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.245
28000
-
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
1.1.1.245
60000
-
gel filtration, isoenzyme II
Rhodococcus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.245
9.8fold, isoenzyme II
Rhodococcus sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.245
127
-
purified enzyme
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.245
1-butanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
butanal + NADH
-
-
-
?
1.1.1.245
1-hexanol + NAD+
relative oxidation activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanal + NADH
-
-
-
?
1.1.1.245
1-pentanol + NAD+
relative activity at 23% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
n-pentanal + NADH
-
-
-
?
1.1.1.245
1-propanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
propionaldehyde + NADH
-
-
-
?
1.1.1.245
2-butanol + NAD+
relative activity at 44% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-butanone + NADH
-
-
-
?
1.1.1.245
2-methyl-1-butanol + NAD+
relative activity at 26% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-methyl-1-butanal + NADH
-
-
-
?
1.1.1.245
2-methyl-1-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
isobutyraldehyde + NADH
-
-
-
?
1.1.1.245
2-methyl-2-propanol + NAD+
relative activity at 16% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
1.1.1.245
2-propanol + NAD+
relative activity at 17% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetone + NADH
-
-
-
?
1.1.1.245
cis-cyclopentane-1,2-diol + NAD+
relative activity at 27% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis/trans mixture at 123% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of trans-isomer at 146% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexan-1,2-diol + NAD+
relative oxidation activity of cis-isomer at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclohexane-1,2-dione + NADH
-
-
-
?
1.1.1.245
cyclohexane-1,3-diol + NAD+
relative oxidation activity at 28% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,3-dione + NADH
-
-
-
?
1.1.1.245
cyclohexane-1,4-diol + NAD+
relative oxidation activity at 22% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclohexane-1,4-dione + NADH
-
-
-
?
1.1.1.245
cyclohexanol + NAD+
-
286383
Rhodococcus sp.
cyclohexanone + NADH
-
-
-
?
1.1.1.245
cyclohexanol + NAD+
-
286383
Rhodococcus sp. TK6
cyclohexanone + NADH
-
-
-
?
1.1.1.245
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
cyclopentanone + NADH
-
-
-
?
1.1.1.245
cyclopentanol + NAD+
relative oxidation activity at 77% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
cyclopentanone + NADH
-
-
-
?
1.1.1.245
ethane-1,2-diol + NAD+
relative activity at 5% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
glyoxal + NADH
-
-
-
?
1.1.1.245
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
acetaldehyde + NADH
-
-
-
?
1.1.1.245
ethanol + NAD+
relative activity at 20% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
acetaldehyde + NADH
-
-
-
?
1.1.1.245
hexane-1,2-diol + NAD+
relative activity at 84% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
2-oxohexanal + NADH
-
-
-
?
1.1.1.245
hexane-1,6-diol + NAD+
relative activity at 21% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
hexanedial + NADH
-
-
-
?
1.1.1.245
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
formaldehyde + NADH + H+
-
-
-
?
1.1.1.245
methanol + NAD+
relative activity at 2% the rate of cyclohexanol oxidation
286383
Rhodococcus sp. TK6
formaldehyde + NADH + H+
-
-
-
?
1.1.1.245
propane-1,2-diol + NAD+
relative activity at 30% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
methylglyoxal + NADH
-
-
-
?
1.1.1.245
propane-1,3-diol + NAD+
relative activity at 7% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
1.1.1.245
trans-cyclopentane-1,2-diol + NAD+
relative activity at 114% the rate of cyclohexanol oxidation
286383
Rhodococcus sp.
? + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.245
dimer
2 * 28000, SDS-PAGE, isoenzyme II
Rhodococcus sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.245
4
-
labile
Rhodococcus sp.
1.1.1.245
40
-
90% activity after incubation for 20 min
Rhodococcus sp.
1.1.1.245
50
-
less than 20% activity after incubation for 20 min
Rhodococcus sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.245
7.5
8.5
stable
Rhodococcus sp.