EC Number | General Stability | Organism |
---|---|---|
1.1.1.244 | dilution inactivates methanol dehydrogenase activity, not reductase activity, sucrose, 20% w/v, stabilizes formaldehyde reductase not methanol dehydrogenase activity, metal ions, such as Fe2+ and Zn2+, do not stabilize methanol dehydrogenase activity, glycerol does not stabilize methanol dehydrogenase activity, dithiothreitol stabilizes reductase, not methanol dehydrogenase activity, storage leads to dissociation | Bacillus methanolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.244 | additional information | - |
additional information | 3.8 mM and 166 mM, methanol, due to two active sites biphasic kinetics for methanol, ethanol or NAD+ in cell-free extracts, not for formaldehyde | Bacillus methanolicus | |
1.1.1.244 | 2 | - |
formaldehyde | - |
Bacillus methanolicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.244 | cytoplasm | - |
Bacillus methanolicus | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 43000 | - |
10 * 43000, SDS-PAGE | Bacillus methanolicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.244 | ethanol + NAD+ | Bacillus methanolicus | - |
ethanal + NADH | - |
? | |
1.1.1.244 | methanol + NAD+ | Bacillus methanolicus | involved in initial methanol oxidation | formaldehyde + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.244 | Bacillus methanolicus | - |
thermotolerant methylotrophic strain C1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.244 | Q-Sepharose, Phenyl-Superose, dehydrogenase activity is almost completely lost after purification, 34% reductase activity are recovered | Bacillus methanolicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 19.6 | - |
formaldehyde reductase activity | Bacillus methanolicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.244 | -80 to 4°C, in the presence of DTT at least 4 days stable | Bacillus methanolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.244 | ethanol + NAD+ | - |
Bacillus methanolicus | ethanal + NADH | - |
? | |
1.1.1.244 | ethanol + NAD+ | best substrate | Bacillus methanolicus | ethanal + NADH | - |
r | |
1.1.1.244 | methanol + NAD+ | 33% of activity with ethanol | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | involved in initial methanol oxidation | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | n-butanol + NAD+ | 87% of activity with ethanol | Bacillus methanolicus | n-butanal + NADH | - |
r | |
1.1.1.244 | n-propanol + NAD+ | 71% of activity with ethanol | Bacillus methanolicus | n-propanal + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.244 | decamer | 10 * 43000, SDS-PAGE | Bacillus methanolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 50 | - |
- |
Bacillus methanolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 6.7 | - |
formaldehyde reduction | Bacillus methanolicus |
1.1.1.244 | 9.5 | - |
methanol oxidation | Bacillus methanolicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.244 | additional information | no pyrroloquinoline quinone enzyme | Bacillus methanolicus | |
1.1.1.244 | NAD+ | required, specific for | Bacillus methanolicus |