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Literature summary extracted from

  • van der Werft, M.J.; van der Ven, C.; Barbirato, F.; Eppink, H.M.; de Bont, J.A.M.; van Berkel, W.J.H.
    Stereoselective carveol dehydrogenase from Rhodococcus erythropolis DCL14 (1999), J. Biol. Chem., 274, 26296-26304.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.243 0.041
-
 (4R,6S)-carveol
-
 Rhodococcus erythropolis
1.1.1.243 0.35
-
 (4R,6R)-carveol
-
 Rhodococcus erythropolis
1.1.1.243 0.39
-
 (4S,6S)-carveol
-
 Rhodococcus erythropolis
1.1.1.243 2
-
 (4S,6R)-carveol
-
 Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.243 40000
-
 4 * 40000, SDS-PAGE Rhodococcus erythropolis
1.1.1.243 120000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.243 (4R,6S)-trans-carveol + NAD+ Rhodococcus erythropolis enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known (4R)-carvone + NADH
-
 ir
1.1.1.243 (4R,6S)-trans-carveol + NAD+ Rhodococcus erythropolis DCL14 enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known (4R)-carvone + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.243 Rhodococcus erythropolis
-
 enzyme is induced after growth on limonene or carveol
-
1.1.1.243 Rhodococcus erythropolis DCL14
-
 enzyme is induced after growth on limonene or carveol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.243 octyl Sepharose, gel filtration, hydroxyapatite, anion exchange Rhodococcus erythropolis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.243 (-)-trans-carveol + NADP+ = (-)-carvone + NADPH + H+ steroselective conversion of (6S)-carveol stereomers from a diastereomeric mixture of (4R)- or (4S)-carveol Rhodococcus erythropolis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.243 1.46
-
-
 Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.243 (1R,2R,4S)-limonene-1,2-diol + NAD+ 15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis ?
-
 ir
1.1.1.243 (1S,2S,4R)-limonene-1,2-diol + NAD+ 25% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis ?
-
 ir
1.1.1.243 (4R,6R)-carveol + NADP+
-
 Rhodococcus erythropolis ?
-
 r
1.1.1.243 (4R,6S)-dihydrocarveol + NAD+ 20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis (4R)-carvone + NADH
-
 ir
1.1.1.243 (4R,6S)-trans-carveol + NAD+ 95% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis (4R)-carvone + NADH
-
 ir
1.1.1.243 (4R,6S)-trans-carveol + NAD+ enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known Rhodococcus erythropolis (4R)-carvone + NADH
-
 ir
1.1.1.243 (4R,6S)-trans-carveol + NAD+ 95% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis DCL14 (4R)-carvone + NADH
-
 ir
1.1.1.243 (4R,6S)-trans-carveol + NAD+ enzyme uses dichlorophenolindophenol as artificial electron acceptor, physiological acceptor not known Rhodococcus erythropolis DCL14 (4R)-carvone + NADH
-
 ir
1.1.1.243 (4S,6R)-carveol + NADP+
-
 Rhodococcus erythropolis ?
-
 r
1.1.1.243 (4S,6RS)-carveol + NAD+ enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis (4S)-carvone + NADH
-
 ir
1.1.1.243 (4S,6RS)-carveol + NAD+ enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis DCL14 (4S)-carvone + NADH
-
 ir
1.1.1.243 (4S,6S)-carveol + NADP+
-
 Rhodococcus erythropolis ?
-
 r
1.1.1.243 (4S,6S)-dihydrocarveol + NAD+ 20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis (4S)-carvone + NADH
-
 ir
1.1.1.243 (R)-indanol + NAD+ 13% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis indanone + NADH
-
 ir
1.1.1.243 (S)-indanol + NAD+ 30% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis indanon + NADH
-
 ir
1.1.1.243 2-ethylcyclohexanol + NAD+ 110% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 2-ethylcyclohexanone + NADH
-
 ir
1.1.1.243 2-methylcyclohexanol + NAD+ 100% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 2-methylcyclohexanone + NADH
-
 ir
1.1.1.243 3,3,5,5-tetramethylcyclohexanol + NAD+ 55% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 3,3,5,5-tetramethylcyclohexanone + NADH
-
 ir
1.1.1.243 3,5,5-trimethylcyclohex-2-en-1-ol + NAD+ 115% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 3,5,5-trimethylcyclohex-2-en-1-one + NADH
-
 ir
1.1.1.243 3-methylcyclohexanol + NAD+ 165% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 3-methylcyclohexanone + NADH
-
 ir
1.1.1.243 3-methylcyclohexanol + NAD+ 165% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis DCL14 3-methylcyclohexanone + NADH
-
 ir
1.1.1.243 4-ethylcyclohexanol + NAD+ 40% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 4-ethylcyclohexanone + NADH
-
 ir
1.1.1.243 4-methylcyclohexanol + NAD+ 35% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis 4-methylcyclohexanone + NADH
-
 ir
1.1.1.243 cyclohex-2-ene-1-ol + NAD+ 10% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis cyclohex-2-ene-1-one + NADH
-
 ir
1.1.1.243 cyclohexanol + NAD+ 15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis cyclohexanone + NADH
-
 ir
1.1.1.243 cyclohexanol + NAD+ 15% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis DCL14 cyclohexanone + NADH
-
 ir
1.1.1.243 sobrerol + NAD+ 20% of activity with (4S,6S)-trans-carveol, enzyme uses dichlorophenolindophenol as artificial electron acceptor Rhodococcus erythropolis ?
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.243 tetramer 4 * 40000, SDS-PAGE Rhodococcus erythropolis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.243 50
-
-
 Rhodococcus erythropolis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.243 6 50 gradual increase in specific activity from 0.55 micromol/min mg to 2.85 micromol/min mg Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.243 35
-
 time dependent inactivation above Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.243 5.5
-
 25% of optimal activity at pH 4.6 and 7.0 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.243 NAD+ each monomer of carveol dehydrogenase contains one tightly bound NAD(H) molecule Rhodococcus erythropolis