BRENDA - Enzyme Database

Demonstration of 3alpha(17beta)-hydroxysteroid dehydrogenase distinct from 3alpha-hydroxysteroid dehydrogenase in hamster liver

Ohmura, M.; Hara, A.; Nakagawa, M.; Sawada, H.; Biochem. J. 266, 583-589 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.213
21-hydroxypregn-4-ene-3,20-dione
-
Mesocricetus auratus
1.1.1.213
5beta-Pregnan-3beta-ol-20-one
-
Mesocricetus auratus
1.1.1.213
dexamethasone
-
Mesocricetus auratus
1.1.1.213
Dienstrol
-
Mesocricetus auratus
1.1.1.213
Hexestrol
-
Mesocricetus auratus
1.1.1.213
Medroxyprogesterone acetate
-
Mesocricetus auratus
1.1.1.213
p-chloromercuribenzenesulfonate
-
Mesocricetus auratus
1.1.1.213
p-chloromercuribenzoate
-
Mesocricetus auratus
1.1.1.213
stilboestrol
-
Mesocricetus auratus
1.1.1.239
21-hydroxypregn-4-ene-3,20-dione
0.010 mM, 73% inhibition
Mesocricetus auratus
1.1.1.239
5alpha-Androstan-3beta-ol-17-one
0.005 mM, 55% inhibition
Mesocricetus auratus
1.1.1.239
5alpha-pregnan-3beta-ol-20-one
0.005 mM, 52% inhibition
Mesocricetus auratus
1.1.1.239
5beta-androstan-3beta-ol-17-one
0.005 mM, 87% inhibition
Mesocricetus auratus
1.1.1.239
5beta-Pregnan-3beta-ol-20-one
0.005 mM, 81% inhibition
Mesocricetus auratus
1.1.1.239
dexamethasone
0.10 mM, 39% inhibition
Mesocricetus auratus
1.1.1.239
Dienstrol
0.010 mM, 60% inhibition
Mesocricetus auratus
1.1.1.239
Hexoestrol
0.010 mM, 62% inhibition
Mesocricetus auratus
1.1.1.239
indomethacin
0.10 mM, 67% inhibition
Mesocricetus auratus
1.1.1.239
Medroxyprogesterone acetate
0.010 mM, 22% inhibition
Mesocricetus auratus
1.1.1.239
stilboestrol
0.010 mM, 61% inhibition
Mesocricetus auratus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.213
0.003
-
NADP+
-
Mesocricetus auratus
1.1.1.213
0.005
-
NADPH
-
Mesocricetus auratus
1.1.1.239
0.0005
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.0022
-
testosterone
-
Mesocricetus auratus
1.1.1.239
0.003
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.0031
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239
0.0038
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.239
0.0046
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.239
0.0048
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239
0.0049
-
5beta-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.239
0.0062
-
5beta-pregnan-3alpha,17beta-diol-20-one
-
Mesocricetus auratus
1.1.1.239
0.0066
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.239
0.0075
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.239
0.02
-
NADH
-
Mesocricetus auratus
1.1.1.239
0.27
-
NAD+
-
Mesocricetus auratus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.213
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.213
35000
-
gel filtration
Mesocricetus auratus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.213
Mesocricetus auratus
-
golden hamster, adult, male
-
1.1.1.239
Mesocricetus auratus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.213
ammonium sulfate precipitation, Q-Sepharose, Red A, HA-Ultrogel, Sephadex G-100, copurification with NAD+ dependent enzyme
Mesocricetus auratus
1.1.1.239
-
Mesocricetus auratus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.213
liver
-
Mesocricetus auratus
-
1.1.1.239
liver
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.213
0.71
-
NADP+ dependent
Mesocricetus auratus
1.1.1.213
4.2
-
NAD+ dependent
Mesocricetus auratus
1.1.1.213
6.2
-
testis
Mesocricetus auratus
1.1.1.213
9.8
-
kidney
Mesocricetus auratus
1.1.1.213
26.8
375
liver
Mesocricetus auratus
1.1.1.239
4.2
-
-
Mesocricetus auratus
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.213
4°C, 10 mM-Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 20% glycerol
Mesocricetus auratus
1.1.1.239
4°C, 10 mM Tris/HCl buffer, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA
Mesocricetus auratus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.213
3alpha-hydroxy-5alpha-androstan-17-one + NADP+
-
286354
Mesocricetus auratus
5alpha-androstan-3,17-dione + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5alpha-androstan-17beta-ol-3-one + NADPH + H+
-
286354
Mesocricetus auratus
5alpha-androstan-3alpha,17beta-diol + NADP+
-
286354
Mesocricetus auratus
?
1.1.1.213
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
286354
Mesocricetus auratus
5beta-androstan-3alpha,17beta-diol + NAD+
-
-
-
?
1.1.1.213
5alpha-androstane-3alpha,17beta-diol + NADP+
-
286354
Mesocricetus auratus
5alpha-androstane-17beta-ol-3-one + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5alpha-pregnan-3alpha,21-diol-20-one + NADP+
-
286354
Mesocricetus auratus
5alpha-pregnan-21-ol-3,20-dione + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5beta-androstan-3alpha-ol-17-one + NADP+
-
286354
Mesocricetus auratus
5beta-androstan-3,17-dione + NADPH
-
286354
Mesocricetus auratus
?
1.1.1.213
5beta-androstane-3alpha,17beta-diol + NADP+
-
286354
Mesocricetus auratus
5beta-androstane-17beta-ol-3-one + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5beta-dihydrocortisone + NADPH
-
286354
Mesocricetus auratus
3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
286354
Mesocricetus auratus
?
1.1.1.213
5beta-pregnan-3alpha-ol-20-one + NADP+
-
286354
Mesocricetus auratus
5beta-pregnane-3,20-dione + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
benzenedihydrodiol + NAD(P)+
-
286354
Mesocricetus auratus
? + NAD(P)+
-
286354
Mesocricetus auratus
?
1.1.1.213
glycochenodeoxycholic acid + NADP+
-
286354
Mesocricetus auratus
7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADPH
-
286354
Mesocricetus auratus
?
1.1.1.213
glycolithocholic acid + NADP+
-
286354
Mesocricetus auratus
3-oxo-5beta-cholanoyl glycine + NADPH
-
286354
Mesocricetus auratus
?
1.1.1.213
lithocholic acid + NADP+
-
286354
Mesocricetus auratus
dehydrolithocholic acid + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.239
5alpha-androstan-3alpha,17beta-diol + NAD+
-
286354
Mesocricetus auratus
5alpha-androstan-3alpha-ol-17-one + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5alpha-pregnan-3,21alpha-diol-20-one + NAD+
-
286354
Mesocricetus auratus
5alpha-pregnan-21-ol-3,20-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-androstan-17beta-ol-3-one + NAD+
-
286354
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-androstan-3alpha,17beta-diol + NAD+
-
286354
Mesocricetus auratus
5beta-androstan-3alpha-ol-17-one + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-pregnan-3alpha,21-diol-20-one + NAD+
-
286354
Mesocricetus auratus
5beta-pregnan-21-ol-3,20-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-pregnan-3alpha-ol-20-one + NAD+
-
286354
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
glycochenodeoxycholic acid + NAD+
-
286354
Mesocricetus auratus
7alpha-hydroxy-3-oxo-5-beta-cholanoyl glycine + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
glycolithocholic acid + NAD+
-
286354
Mesocricetus auratus
3-oxo-5beta-cholanoyl glycine + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
lithocholic acid + NAD+
-
286354
Mesocricetus auratus
5beta-cholan-3-one-24-oic acid + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
testosterone + NAD+
-
286354
Mesocricetus auratus
androst-4-ene-3,17-dione + NADH + H+
-
286354
Mesocricetus auratus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.213
monomer
1 * 35000 SDS-PAGE
Mesocricetus auratus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.213
25
-
assay at
Mesocricetus auratus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.213
45
-
50% inactivation after 17 min
Mesocricetus auratus
1.1.1.239
45
-
half-life 2.3 min
Mesocricetus auratus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.213
0.2
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.213
0.217
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.213
0.233
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.213
0.233
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.213
0.35
-
5alpha-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.213
0.417
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.213
0.433
-
5beta-Dihydrocortisone
-
Mesocricetus auratus
1.1.1.213
0.45
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.213
0.567
-
5alpha-Androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.213
0.633
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.213
0.733
-
Dehydrolithocholic acid
-
Mesocricetus auratus
1.1.1.213
0.767
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.213
0.767
-
5alpha-pregnan-21-ol-3,20-one
-
Mesocricetus auratus
1.1.1.213
1.2
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.213
1.33
-
5beta-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.1.1.213
1.33
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.213
2.72
-
5alpha-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.1.1.239
0.02
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.239
0.05
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.158
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.239
0.167
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.239
0.233
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.239
0.25
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.267
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.239
0.367
-
5beta-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.239
0.367
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.239
0.417
-
testosterone
-
Mesocricetus auratus
1.1.1.239
0.45
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239
0.85
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.239
10.4
-
oxidation of 5beta-androstan-3alpha,17beta-diol
Mesocricetus auratus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.213
NADP+
-
Mesocricetus auratus
1.1.1.213
NADPH
-
Mesocricetus auratus
1.1.1.239
NAD+
preferred
Mesocricetus auratus
1.1.1.239
NADH
preferred
Mesocricetus auratus
1.1.1.239
NADP+
15% of NAD+-activity
Mesocricetus auratus
1.1.1.239
NADPH
-
Mesocricetus auratus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.213
NADP+
-
Mesocricetus auratus
1.1.1.213
NADPH
-
Mesocricetus auratus
1.1.1.239
NAD+
preferred
Mesocricetus auratus
1.1.1.239
NADH
preferred
Mesocricetus auratus
1.1.1.239
NADP+
15% of NAD+-activity
Mesocricetus auratus
1.1.1.239
NADPH
-
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.213
21-hydroxypregn-4-ene-3,20-dione
-
Mesocricetus auratus
1.1.1.213
5beta-Pregnan-3beta-ol-20-one
-
Mesocricetus auratus
1.1.1.213
dexamethasone
-
Mesocricetus auratus
1.1.1.213
Dienstrol
-
Mesocricetus auratus
1.1.1.213
Hexestrol
-
Mesocricetus auratus
1.1.1.213
Medroxyprogesterone acetate
-
Mesocricetus auratus
1.1.1.213
p-chloromercuribenzenesulfonate
-
Mesocricetus auratus
1.1.1.213
p-chloromercuribenzoate
-
Mesocricetus auratus
1.1.1.213
stilboestrol
-
Mesocricetus auratus
1.1.1.239
21-hydroxypregn-4-ene-3,20-dione
0.010 mM, 73% inhibition
Mesocricetus auratus
1.1.1.239
5alpha-Androstan-3beta-ol-17-one
0.005 mM, 55% inhibition
Mesocricetus auratus
1.1.1.239
5alpha-pregnan-3beta-ol-20-one
0.005 mM, 52% inhibition
Mesocricetus auratus
1.1.1.239
5beta-androstan-3beta-ol-17-one
0.005 mM, 87% inhibition
Mesocricetus auratus
1.1.1.239
5beta-Pregnan-3beta-ol-20-one
0.005 mM, 81% inhibition
Mesocricetus auratus
1.1.1.239
dexamethasone
0.10 mM, 39% inhibition
Mesocricetus auratus
1.1.1.239
Dienstrol
0.010 mM, 60% inhibition
Mesocricetus auratus
1.1.1.239
Hexoestrol
0.010 mM, 62% inhibition
Mesocricetus auratus
1.1.1.239
indomethacin
0.10 mM, 67% inhibition
Mesocricetus auratus
1.1.1.239
Medroxyprogesterone acetate
0.010 mM, 22% inhibition
Mesocricetus auratus
1.1.1.239
stilboestrol
0.010 mM, 61% inhibition
Mesocricetus auratus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.213
0.003
-
NADP+
-
Mesocricetus auratus
1.1.1.213
0.005
-
NADPH
-
Mesocricetus auratus
1.1.1.239
0.0005
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.0022
-
testosterone
-
Mesocricetus auratus
1.1.1.239
0.003
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.0031
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239
0.0038
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.239
0.0046
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.239
0.0048
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239
0.0049
-
5beta-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.239
0.0062
-
5beta-pregnan-3alpha,17beta-diol-20-one
-
Mesocricetus auratus
1.1.1.239
0.0066
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.239
0.0075
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.239
0.02
-
NADH
-
Mesocricetus auratus
1.1.1.239
0.27
-
NAD+
-
Mesocricetus auratus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.213
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.213
35000
-
gel filtration
Mesocricetus auratus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.213
ammonium sulfate precipitation, Q-Sepharose, Red A, HA-Ultrogel, Sephadex G-100, copurification with NAD+ dependent enzyme
Mesocricetus auratus
1.1.1.239
-
Mesocricetus auratus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.213
liver
-
Mesocricetus auratus
-
1.1.1.239
liver
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.213
0.71
-
NADP+ dependent
Mesocricetus auratus
1.1.1.213
4.2
-
NAD+ dependent
Mesocricetus auratus
1.1.1.213
6.2
-
testis
Mesocricetus auratus
1.1.1.213
9.8
-
kidney
Mesocricetus auratus
1.1.1.213
26.8
375
liver
Mesocricetus auratus
1.1.1.239
4.2
-
-
Mesocricetus auratus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.213
4°C, 10 mM-Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 20% glycerol
Mesocricetus auratus
1.1.1.239
4°C, 10 mM Tris/HCl buffer, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.213
3alpha-hydroxy-5alpha-androstan-17-one + NADP+
-
286354
Mesocricetus auratus
5alpha-androstan-3,17-dione + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5alpha-androstan-17beta-ol-3-one + NADPH + H+
-
286354
Mesocricetus auratus
5alpha-androstan-3alpha,17beta-diol + NADP+
-
286354
Mesocricetus auratus
?
1.1.1.213
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
286354
Mesocricetus auratus
5beta-androstan-3alpha,17beta-diol + NAD+
-
-
-
?
1.1.1.213
5alpha-androstane-3alpha,17beta-diol + NADP+
-
286354
Mesocricetus auratus
5alpha-androstane-17beta-ol-3-one + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5alpha-pregnan-3alpha,21-diol-20-one + NADP+
-
286354
Mesocricetus auratus
5alpha-pregnan-21-ol-3,20-dione + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5beta-androstan-3alpha-ol-17-one + NADP+
-
286354
Mesocricetus auratus
5beta-androstan-3,17-dione + NADPH
-
286354
Mesocricetus auratus
?
1.1.1.213
5beta-androstane-3alpha,17beta-diol + NADP+
-
286354
Mesocricetus auratus
5beta-androstane-17beta-ol-3-one + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
5beta-dihydrocortisone + NADPH
-
286354
Mesocricetus auratus
3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
286354
Mesocricetus auratus
?
1.1.1.213
5beta-pregnan-3alpha-ol-20-one + NADP+
-
286354
Mesocricetus auratus
5beta-pregnane-3,20-dione + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.213
benzenedihydrodiol + NAD(P)+
-
286354
Mesocricetus auratus
? + NAD(P)+
-
286354
Mesocricetus auratus
?
1.1.1.213
glycochenodeoxycholic acid + NADP+
-
286354
Mesocricetus auratus
7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADPH
-
286354
Mesocricetus auratus
?
1.1.1.213
glycolithocholic acid + NADP+
-
286354
Mesocricetus auratus
3-oxo-5beta-cholanoyl glycine + NADPH
-
286354
Mesocricetus auratus
?
1.1.1.213
lithocholic acid + NADP+
-
286354
Mesocricetus auratus
dehydrolithocholic acid + NADPH
-
286354
Mesocricetus auratus
r
1.1.1.239
5alpha-androstan-3alpha,17beta-diol + NAD+
-
286354
Mesocricetus auratus
5alpha-androstan-3alpha-ol-17-one + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5alpha-pregnan-3,21alpha-diol-20-one + NAD+
-
286354
Mesocricetus auratus
5alpha-pregnan-21-ol-3,20-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-androstan-17beta-ol-3-one + NAD+
-
286354
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-androstan-3alpha,17beta-diol + NAD+
-
286354
Mesocricetus auratus
5beta-androstan-3alpha-ol-17-one + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-pregnan-3alpha,21-diol-20-one + NAD+
-
286354
Mesocricetus auratus
5beta-pregnan-21-ol-3,20-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
5beta-pregnan-3alpha-ol-20-one + NAD+
-
286354
Mesocricetus auratus
5beta-pregnan-3,20-dione + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
glycochenodeoxycholic acid + NAD+
-
286354
Mesocricetus auratus
7alpha-hydroxy-3-oxo-5-beta-cholanoyl glycine + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
glycolithocholic acid + NAD+
-
286354
Mesocricetus auratus
3-oxo-5beta-cholanoyl glycine + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
lithocholic acid + NAD+
-
286354
Mesocricetus auratus
5beta-cholan-3-one-24-oic acid + NADH
-
286354
Mesocricetus auratus
?
1.1.1.239
testosterone + NAD+
-
286354
Mesocricetus auratus
androst-4-ene-3,17-dione + NADH + H+
-
286354
Mesocricetus auratus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.213
monomer
1 * 35000 SDS-PAGE
Mesocricetus auratus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.213
25
-
assay at
Mesocricetus auratus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.213
45
-
50% inactivation after 17 min
Mesocricetus auratus
1.1.1.239
45
-
half-life 2.3 min
Mesocricetus auratus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.213
0.2
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.213
0.217
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.213
0.233
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.213
0.233
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.213
0.35
-
5alpha-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.213
0.417
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.213
0.433
-
5beta-Dihydrocortisone
-
Mesocricetus auratus
1.1.1.213
0.45
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.213
0.567
-
5alpha-Androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.213
0.633
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.213
0.733
-
Dehydrolithocholic acid
-
Mesocricetus auratus
1.1.1.213
0.767
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.213
0.767
-
5alpha-pregnan-21-ol-3,20-one
-
Mesocricetus auratus
1.1.1.213
1.2
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.213
1.33
-
5beta-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.1.1.213
1.33
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.213
2.72
-
5alpha-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.1.1.239
0.02
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.239
0.05
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.158
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.239
0.167
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.239
0.233
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.239
0.25
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.239
0.267
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.239
0.367
-
5beta-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.239
0.367
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.239
0.417
-
testosterone
-
Mesocricetus auratus
1.1.1.239
0.45
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239
0.85
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.239
10.4
-
oxidation of 5beta-androstan-3alpha,17beta-diol
Mesocricetus auratus