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Literature summary extracted from

  • Ohmura, M.; Hara, A.; Nakagawa, M.; Sawada, H.
    Demonstration of 3alpha(17beta)-hydroxysteroid dehydrogenase distinct from 3alpha-hydroxysteroid dehydrogenase in hamster liver (1990), Biochem. J., 266, 583-589.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.213 21-hydroxypregn-4-ene-3,20-dione
-
Mesocricetus auratus
1.1.1.213 5beta-Pregnan-3beta-ol-20-one
-
Mesocricetus auratus
1.1.1.213 dexamethasone
-
Mesocricetus auratus
1.1.1.213 Dienstrol
-
Mesocricetus auratus
1.1.1.213 Hexestrol
-
Mesocricetus auratus
1.1.1.213 Medroxyprogesterone acetate
-
Mesocricetus auratus
1.1.1.213 p-chloromercuribenzenesulfonate
-
Mesocricetus auratus
1.1.1.213 p-chloromercuribenzoate
-
Mesocricetus auratus
1.1.1.213 stilboestrol
-
Mesocricetus auratus
1.1.1.239 21-hydroxypregn-4-ene-3,20-dione 0.010 mM, 73% inhibition Mesocricetus auratus
1.1.1.239 5alpha-Androstan-3beta-ol-17-one 0.005 mM, 55% inhibition Mesocricetus auratus
1.1.1.239 5alpha-pregnan-3beta-ol-20-one 0.005 mM, 52% inhibition Mesocricetus auratus
1.1.1.239 5beta-androstan-3beta-ol-17-one 0.005 mM, 87% inhibition Mesocricetus auratus
1.1.1.239 5beta-Pregnan-3beta-ol-20-one 0.005 mM, 81% inhibition Mesocricetus auratus
1.1.1.239 dexamethasone 0.10 mM, 39% inhibition Mesocricetus auratus
1.1.1.239 Dienstrol 0.010 mM, 60% inhibition Mesocricetus auratus
1.1.1.239 Hexoestrol 0.010 mM, 62% inhibition Mesocricetus auratus
1.1.1.239 indomethacin 0.10 mM, 67% inhibition Mesocricetus auratus
1.1.1.239 Medroxyprogesterone acetate 0.010 mM, 22% inhibition Mesocricetus auratus
1.1.1.239 stilboestrol 0.010 mM, 61% inhibition Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.213 0.003
-
NADP+
-
Mesocricetus auratus
1.1.1.213 0.005
-
NADPH
-
Mesocricetus auratus
1.1.1.239 0.0005
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.239 0.0022
-
testosterone
-
Mesocricetus auratus
1.1.1.239 0.003
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.239 0.0031
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239 0.0038
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.239 0.0046
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.239 0.0048
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239 0.0049
-
5beta-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.239 0.0062
-
5beta-pregnan-3alpha,17beta-diol-20-one
-
Mesocricetus auratus
1.1.1.239 0.0066
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.239 0.0075
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.239 0.02
-
NADH
-
Mesocricetus auratus
1.1.1.239 0.27
-
NAD+
-
Mesocricetus auratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.213 cytosol
-
Mesocricetus auratus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.213 35000
-
gel filtration Mesocricetus auratus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.213 Mesocricetus auratus
-
golden hamster, adult, male
-
1.1.1.239 Mesocricetus auratus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.213 ammonium sulfate precipitation, Q-Sepharose, Red A, HA-Ultrogel, Sephadex G-100, copurification with NAD+ dependent enzyme Mesocricetus auratus
1.1.1.239
-
Mesocricetus auratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.213 liver
-
Mesocricetus auratus
-
1.1.1.239 liver
-
Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.213 0.71
-
NADP+ dependent Mesocricetus auratus
1.1.1.213 4.2
-
NAD+ dependent Mesocricetus auratus
1.1.1.213 6.2
-
testis Mesocricetus auratus
1.1.1.213 9.8
-
kidney Mesocricetus auratus
1.1.1.213 26.8 375 liver Mesocricetus auratus
1.1.1.239 4.2
-
-
Mesocricetus auratus

Storage Stability

EC Number Storage Stability Organism
1.1.1.213 4°C, 10 mM-Tris/HCl, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 20% glycerol Mesocricetus auratus
1.1.1.239 4°C, 10 mM Tris/HCl buffer, pH 8.0, 5 mM 2-mercaptoethanol, 0.5 mM EDTA Mesocricetus auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.213 3alpha-hydroxy-5alpha-androstan-17-one + NADP+
-
Mesocricetus auratus 5alpha-androstan-3,17-dione + NADPH
-
r
1.1.1.213 5alpha-androstan-17beta-ol-3-one + NADPH + H+
-
Mesocricetus auratus 5alpha-androstan-3alpha,17beta-diol + NADP+
-
?
1.1.1.213 5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
Mesocricetus auratus 5beta-androstan-3alpha,17beta-diol + NAD+
-
?
1.1.1.213 5alpha-androstane-3alpha,17beta-diol + NADP+
-
Mesocricetus auratus 5alpha-androstane-17beta-ol-3-one + NADPH
-
r
1.1.1.213 5alpha-pregnan-3alpha,21-diol-20-one + NADP+
-
Mesocricetus auratus 5alpha-pregnan-21-ol-3,20-dione + NADPH
-
r
1.1.1.213 5beta-androstan-3alpha-ol-17-one + NADP+
-
Mesocricetus auratus 5beta-androstan-3,17-dione + NADPH
-
?
1.1.1.213 5beta-androstane-3alpha,17beta-diol + NADP+
-
Mesocricetus auratus 5beta-androstane-17beta-ol-3-one + NADPH
-
r
1.1.1.213 5beta-dihydrocortisone + NADPH
-
Mesocricetus auratus 3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
?
1.1.1.213 5beta-pregnan-3alpha-ol-20-one + NADP+
-
Mesocricetus auratus 5beta-pregnane-3,20-dione + NADPH
-
r
1.1.1.213 benzenedihydrodiol + NAD(P)+
-
Mesocricetus auratus ?
-
?
1.1.1.213 glycochenodeoxycholic acid + NADP+
-
Mesocricetus auratus 7alpha-hydroxy-3-oxo-5beta-cholanoyl glycine + NADPH
-
?
1.1.1.213 glycolithocholic acid + NADP+
-
Mesocricetus auratus 3-oxo-5beta-cholanoyl glycine + NADPH
-
?
1.1.1.213 lithocholic acid + NADP+
-
Mesocricetus auratus dehydrolithocholic acid + NADPH
-
r
1.1.1.239 5alpha-androstan-3alpha,17beta-diol + NAD+
-
Mesocricetus auratus 5alpha-androstan-3alpha-ol-17-one + NADH
-
?
1.1.1.239 5alpha-pregnan-3,21alpha-diol-20-one + NAD+
-
Mesocricetus auratus 5alpha-pregnan-21-ol-3,20-dione + NADH
-
?
1.1.1.239 5beta-androstan-17beta-ol-3-one + NAD+
-
Mesocricetus auratus 5beta-androstan-3,17-dione + NADH
-
?
1.1.1.239 5beta-androstan-3alpha,17beta-diol + NAD+
-
Mesocricetus auratus 5beta-androstan-3alpha-ol-17-one + NADH
-
?
1.1.1.239 5beta-pregnan-3alpha,21-diol-20-one + NAD+
-
Mesocricetus auratus 5beta-pregnan-21-ol-3,20-dione + NADH
-
?
1.1.1.239 5beta-pregnan-3alpha-ol-20-one + NAD+
-
Mesocricetus auratus 5beta-pregnan-3,20-dione + NADH
-
?
1.1.1.239 glycochenodeoxycholic acid + NAD+
-
Mesocricetus auratus 7alpha-hydroxy-3-oxo-5-beta-cholanoyl glycine + NADH
-
?
1.1.1.239 glycolithocholic acid + NAD+
-
Mesocricetus auratus 3-oxo-5beta-cholanoyl glycine + NADH
-
?
1.1.1.239 lithocholic acid + NAD+
-
Mesocricetus auratus 5beta-cholan-3-one-24-oic acid + NADH
-
?
1.1.1.239 testosterone + NAD+
-
Mesocricetus auratus androst-4-ene-3,17-dione + NADH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.213 monomer 1 * 35000 SDS-PAGE Mesocricetus auratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.213 25
-
assay at Mesocricetus auratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.213 45
-
50% inactivation after 17 min Mesocricetus auratus
1.1.1.239 45
-
half-life 2.3 min Mesocricetus auratus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.213 0.2
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.213 0.217
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.213 0.233
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.213 0.233
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.213 0.35
-
5alpha-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.213 0.417
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.213 0.433
-
5beta-Dihydrocortisone
-
Mesocricetus auratus
1.1.1.213 0.45
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.213 0.567
-
5alpha-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.213 0.633
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.213 0.733
-
Dehydrolithocholic acid
-
Mesocricetus auratus
1.1.1.213 0.767
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.213 0.767
-
5alpha-pregnan-21-ol-3,20-one
-
Mesocricetus auratus
1.1.1.213 1.2
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.213 1.33
-
5beta-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.1.1.213 1.33
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.213 2.72
-
5alpha-Androstan-3alpha-ol-17-one
-
Mesocricetus auratus
1.1.1.239 0.02
-
glycochenodeoxycholic acid
-
Mesocricetus auratus
1.1.1.239 0.05
-
lithocholic acid
-
Mesocricetus auratus
1.1.1.239 0.158
-
5beta-pregnan-3,20-dione
-
Mesocricetus auratus
1.1.1.239 0.167
-
5beta-androstan-3,17-dione
-
Mesocricetus auratus
1.1.1.239 0.233
-
5beta-Pregnan-3alpha-ol-20-one
-
Mesocricetus auratus
1.1.1.239 0.25
-
glycolithocholic acid
-
Mesocricetus auratus
1.1.1.239 0.267
-
5alpha-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.239 0.367
-
5beta-androstan-17beta-ol-3-one
-
Mesocricetus auratus
1.1.1.239 0.367
-
5beta-Pregnan-3alpha,21-diol-20-one
-
Mesocricetus auratus
1.1.1.239 0.417
-
testosterone
-
Mesocricetus auratus
1.1.1.239 0.45
-
5alpha-androstan-3alpha,17beta-diol
-
Mesocricetus auratus
1.1.1.239 0.85
-
5beta-Androstan-3alpha,17beta-diol
-
Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.239 10.4
-
oxidation of 5beta-androstan-3alpha,17beta-diol Mesocricetus auratus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.213 NADP+
-
Mesocricetus auratus
1.1.1.213 NADPH
-
Mesocricetus auratus
1.1.1.239 NAD+ preferred Mesocricetus auratus
1.1.1.239 NADH preferred Mesocricetus auratus
1.1.1.239 NADP+ 15% of NAD+-activity Mesocricetus auratus
1.1.1.239 NADPH
-
Mesocricetus auratus