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Literature summary extracted from

  • Nagai, A.; Ohta, D.
    Histidinol dehydrogenase loses its catalytic function through the mutation of His261-Asn due to its inability to ligate the essential Zn (1994), J. Biochem., 115, 22-25.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.23 expressed in Spodoptera frugiperda, SF9 cells Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.23 H261N mutation causes complete loss of Zn and activity Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.23 0.0027
-
 L-histidinal
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.23 0.0037
-
 L-histidinal H216N mutation Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.23 0.0072
-
 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.23 0.24
-
 NAD+ H216N mutation Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.23 Zn2+ essential for activity, one Zn atom per subunit Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.23 L-histidinal + NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium reaction intermediate L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinol + 2 NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.23 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.23 L-histidinal + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinal + NAD+ + H2O reaction intermediate Salmonella enterica subsp. enterica serovar Typhimurium L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinol + 2 NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-histidine + 2 NADH + 2 H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.23 0.15
-
 L-histidinal H216N mutation Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.23 3.6
-
 L-histidinal
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.23 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium