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Literature summary extracted from

  • Yamano, S.; Ito, K.; Ogata, S.; Toki, S.
    Purification, characterization and partial primary structure of morphine 6-dehydrogenase from rabbit liver cytosol (1997), Arch. Biochem. Biophys., 341, 81-88.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.218 enzyme stable without 2-mercaptoethanol Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.218 5beta-dihydrotestosterone
-
 Oryctolagus cuniculus
1.1.1.218 additional information 5beta-dihydrotestosterone, lithocholic acid, androsterone and estradiol inhibit competitively toward morphine binding; little inhibitory effect: indomethacin, prostaglandins E2, D2; no or weak inhibition by pyrazole, barbital Oryctolagus cuniculus
1.1.1.218 NEM
-
 Oryctolagus cuniculus
1.1.1.218 p-chloromercuribenzoic acid
-
 Oryctolagus cuniculus
1.1.1.218 Phenylarsine oxide
-
 Oryctolagus cuniculus
1.1.1.218 quercetin
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.218 0.07
-
 codeine with NAD+ Oryctolagus cuniculus
1.1.1.218 0.12
-
 1-Indanol cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 0.39
-
 Dihydrocodeine cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 0.54
-
 cyclohexene-2-ol cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 0.64
-
 normorphine cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 0.72
-
 morphine cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 0.92
-
 3-hydroxyhexobarbital cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 1.48
-
 ethylmorphine cosubstrate NAD+ Oryctolagus cuniculus
1.1.1.218 1.75
-
 benzenedihydrodiol cosubstrate NAD+ Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.218 cytosol
-
 Oryctolagus cuniculus 5829
-
1.1.1.218 microsome microsomal fraction Oryctolagus cuniculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.218 31000
-
 native enzyme, gel filtration Oryctolagus cuniculus
1.1.1.218 36000
-
 denatured enzyme, SDS-PAGE Oryctolagus cuniculus
1.1.1.218 38000
-
 1 * 38000, SDS-PAGE Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.218 Oryctolagus cuniculus
-
 Japanese white rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.218 gel filtration, various chromatographic techniques Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.218 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.218 0.43
-
 morphine, NAD+ Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.1.1.218 -80°C, 20% glycerol, v/v, stable at least four months Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.218 1-indanol + NADP+
-
 Oryctolagus cuniculus 1-indanone + NADPH
-
 ?
1.1.1.218 3-hydroxyhexobarbital + NADP+ does not serve as a substrate for guinea pig enzyme Oryctolagus cuniculus ?
-
 ?
1.1.1.218 benzenedihydrodiol + NADP+
-
 Oryctolagus cuniculus ?
-
 ?
1.1.1.218 codeine + NAD(P)+ codeine best substrate Oryctolagus cuniculus codeinone + NAD(P)H
-
 ?
1.1.1.218 cyclohexene-2-ol + NADP+
-
 Oryctolagus cuniculus cyclohexene-2-one + NADPH
-
 ?
1.1.1.218 dihydrocodeine + NADP+
-
 Oryctolagus cuniculus dihydrocodeinone + NADPH
-
 ?
1.1.1.218 ethylmorphine + NAD(P)+
-
 Oryctolagus cuniculus ?
-
 ?
1.1.1.218 morphine + NADP+ enzyme acitivity with NAD+ four times that with NADP+ Oryctolagus cuniculus morphinone + NADPH + H+
-
 ?
1.1.1.218 normorphine + NAD(P)+
-
 Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.218 monomer 1 * 38000, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.218 25
-
 enzyme assay Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.218 8.6
-
 with NADP+ as cofactor, in glycine-NaCl-NaOH buffer Oryctolagus cuniculus
1.1.1.218 9.4
-
 with NAD+ as cofactor, in glycine-NaCl-NaOH buffer Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.218 NAD(P)+ NAD+ preferred cofactor Oryctolagus cuniculus