EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.218 | CuSO4 | - |
Cavia porcellus | |
1.1.1.218 | Ketamine | - |
Cavia porcellus | |
1.1.1.218 | lithocholic acid | - |
Cavia porcellus | |
1.1.1.218 | additional information | no or weak inhibition by pyrazole, barbital | Cavia porcellus | |
1.1.1.218 | Phenylarsine oxide | - |
Cavia porcellus | |
1.1.1.218 | prostaglandin E1 | - |
Cavia porcellus | |
1.1.1.218 | quercitrin | - |
Cavia porcellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.218 | 0.0022 | - |
NADH | - |
Cavia porcellus | |
1.1.1.218 | 0.0065 | - |
NADPH | - |
Cavia porcellus | |
1.1.1.218 | 0.27 | - |
naloxone | cosubstrate NADPH | Cavia porcellus | |
1.1.1.218 | 0.44 | - |
naloxone | cosubstrate NADH | Cavia porcellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.218 | cytosol | - |
Cavia porcellus | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.218 | Cavia porcellus | - |
- |
- |
1.1.1.218 | Cavia porcellus Hartley | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.218 | gel filtration, various chromatographic techniques | Cavia porcellus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.218 | liver | - |
Cavia porcellus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 0.86 | - |
morphine, NADP+ | Cavia porcellus |
1.1.1.218 | 0.9 | - |
naloxone, NADPH | Cavia porcellus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.218 | 2-4°C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week | Cavia porcellus |
1.1.1.218 | 4°C, 80% of the activity after a week in 50 mM sodium phosphate buffer, pH 6.0, 100 mM 2-mercaptoethanol, more stable at pH 6.0 than at pH 8.0 | Cavia porcellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.218 | morphine + NADP+ | - |
Cavia porcellus | morphinone + NADPH + H+ | - |
? | |
1.1.1.218 | morphine + NADP+ | - |
Cavia porcellus Hartley | morphinone + NADPH + H+ | - |
? | |
1.1.1.218 | naloxone + NAD(P)H | - |
Cavia porcellus | 6alpha-naloxol + NADP+ | stereospecific reduction | ? | |
1.1.1.218 | naloxone + NAD(P)H | - |
Cavia porcellus Hartley | 6alpha-naloxol + NADP+ | stereospecific reduction | ? | |
1.1.1.218 | naloxone + NADH + H+ | - |
Cavia porcellus | ? | - |
r | |
1.1.1.218 | naloxone + NADH + H+ | - |
Cavia porcellus Hartley | ? | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.218 | naloxone reductase | - |
Cavia porcellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 37 | - |
enzyme assay, reduction of naloxone | Cavia porcellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 6.2 | - |
with NADH als cofactor, 67 mM sodium phosphate buffer | Cavia porcellus |
1.1.1.218 | 6.8 | - |
with NADPH as cofactor, 67 mM sodium phosphate buffer | Cavia porcellus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.218 | 6 | - |
at pH6.0 more stable than at pH 8.0 on storage at 4°C | Cavia porcellus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.218 | NAD(P)+ | utilized both with nearly equal efficiency | Cavia porcellus | |
1.1.1.218 | NAD(P)H | NADPH: 2.7 times as effective as NADH | Cavia porcellus |