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Literature summary extracted from

  • Chiyonobu, T.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Purification, crystallization and properties of 2-ketogluconate reductase from Acetobacter rancens (1976), Agric. Biol. Chem., 40, 175-184.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.215
-
Acetobacter pasteurianus

General Stability

EC Number General Stability Organism
1.1.1.215 2-mercaptoethanol stabilizes Acetobacter pasteurianus
1.1.1.215 dialysis, stable to Acetobacter pasteurianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.215 Cd2+
-
Acetobacter pasteurianus
1.1.1.215 Co2+
-
Acetobacter pasteurianus
1.1.1.215 Hg2+ complete inhibition Acetobacter pasteurianus
1.1.1.215 additional information not inhibited by monoiodoacetate Acetobacter pasteurianus
1.1.1.215 oxalate
-
Acetobacter pasteurianus
1.1.1.215 oxamate slight inhibition of reverse reaction Acetobacter pasteurianus
1.1.1.215 pyruvate weak Acetobacter pasteurianus
1.1.1.215 Tartronate slight inhibition of reverse reaction Acetobacter pasteurianus
1.1.1.215 Zn2+
-
Acetobacter pasteurianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.215 0.01
-
NADPH + 5-keto-D-gluconate Acetobacter pasteurianus
1.1.1.215 0.062
-
NADPH + 2-keto-D-galactonate Acetobacter pasteurianus
1.1.1.215 0.065
-
Hydroxypyruvate
-
Acetobacter pasteurianus
1.1.1.215 0.083
-
NADP+ + D-gluconate Acetobacter pasteurianus
1.1.1.215 0.11
-
NADPH + 2-keto-L-gulonate Acetobacter pasteurianus
1.1.1.215 0.14
-
NADPH + hydroxypyruvate Acetobacter pasteurianus
1.1.1.215 0.38
-
glyoxylate
-
Acetobacter pasteurianus
1.1.1.215 0.71
-
NADPH cosubstrate glyoxylate Acetobacter pasteurianus
1.1.1.215 0.86
-
5-Keto-D-gluconate
-
Acetobacter pasteurianus
1.1.1.215 2.4
-
D-gluconate
-
Acetobacter pasteurianus
1.1.1.215 16
-
2-keto-D-galactonate
-
Acetobacter pasteurianus
1.1.1.215 91
-
2-keto-L-gulonate
-
Acetobacter pasteurianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 cytoplasm
-
Acetobacter pasteurianus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.215 additional information no metal requirement Acetobacter pasteurianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 15000
-
8 * 15000, SDS-PAGE Acetobacter pasteurianus
1.1.1.215 120000
-
gel filtration Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 2-dehydro-D-gluconate + NADPH Acetobacter pasteurianus
-
D-gluconate + NADP+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Acetobacter pasteurianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215
-
Acetobacter pasteurianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.215 336
-
-
Acetobacter pasteurianus

Storage Stability

EC Number Storage Stability Organism
1.1.1.215 -20°C, stable in the presence of sulfhydryl agents and ammonium sulfate Acetobacter pasteurianus
1.1.1.215 0°C, stable for at least a month in phosphate buffer Acetobacter pasteurianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 2-dehydro-D-gluconate + NADPH
-
Acetobacter pasteurianus D-gluconate + NADP+
-
r
1.1.1.215 2-keto-L-gulonate + NADPH
-
Acetobacter pasteurianus L-idonate + NADP+
-
r
1.1.1.215 5-keto-D-gluconate + NADPH poor substrate Acetobacter pasteurianus D-gluconate + NADP+
-
?
1.1.1.215 acetaldehyde + NADPH + H+ poor substrate Acetobacter pasteurianus ethanol + NADP+
-
?
1.1.1.215 D-galactonate + NADP+
-
Acetobacter pasteurianus 2-dehydro-D-galactonate + NADPH
-
r
1.1.1.215 glyoxal + NADPH reduction at 33% the rate of 2-ketogluconate reduction Acetobacter pasteurianus ?
-
?
1.1.1.215 glyoxylate + NADPH reduction at 350% the rate of 2-ketogluconate reduction Acetobacter pasteurianus glycolate + NADP+
-
?
1.1.1.215 hydroxypyruvate + NADPH reduction at 733% the rate of 2-keto-gluconate reduction Acetobacter pasteurianus 2,3-dihydroxypropanoate + NADP+
-
?
1.1.1.215 L-idonate + NADP+
-
Acetobacter pasteurianus 2-keto-L-idonate + NADPH
-
?
1.1.1.215 pyruvate + NADPH + H+ reduction at 7% the rate of 2-ketogluconate reduction Acetobacter pasteurianus lactate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 octamer 8 * 15000, SDS-PAGE Acetobacter pasteurianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.215 50
-
-
Acetobacter pasteurianus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.215 40 55 about half-maximal activity at 40°C and 55°C Acetobacter pasteurianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.215 55
-
stable for 10 min Acetobacter pasteurianus
1.1.1.215 60
-
rapid inactivation Acetobacter pasteurianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.215 7
-
reduction of 2-ketogluconate Acetobacter pasteurianus
1.1.1.215 12
-
-
Acetobacter pasteurianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.215 5 9.2 about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate Acetobacter pasteurianus
1.1.1.215 10.8 12.5 about half-maximal activity at pH 10.8 and 12.5, oxidation of gluconate Acetobacter pasteurianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.215 6
-
stable for a week in the cold, increasing inactivation below and above, stable for a month or more with additional 2-mercaptoethanol Acetobacter pasteurianus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 NADP+
-
Acetobacter pasteurianus
1.1.1.215 NADPH
-
Acetobacter pasteurianus