EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.215 | - |
Acetobacter pasteurianus |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.215 | 2-mercaptoethanol stabilizes | Acetobacter pasteurianus |
1.1.1.215 | dialysis, stable to | Acetobacter pasteurianus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.215 | Cd2+ | - |
Acetobacter pasteurianus | |
1.1.1.215 | Co2+ | - |
Acetobacter pasteurianus | |
1.1.1.215 | Hg2+ | complete inhibition | Acetobacter pasteurianus | |
1.1.1.215 | additional information | not inhibited by monoiodoacetate | Acetobacter pasteurianus | |
1.1.1.215 | oxalate | - |
Acetobacter pasteurianus | |
1.1.1.215 | oxamate | slight inhibition of reverse reaction | Acetobacter pasteurianus | |
1.1.1.215 | pyruvate | weak | Acetobacter pasteurianus | |
1.1.1.215 | Tartronate | slight inhibition of reverse reaction | Acetobacter pasteurianus | |
1.1.1.215 | Zn2+ | - |
Acetobacter pasteurianus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.215 | 0.01 | - |
NADPH | + 5-keto-D-gluconate | Acetobacter pasteurianus | |
1.1.1.215 | 0.062 | - |
NADPH | + 2-keto-D-galactonate | Acetobacter pasteurianus | |
1.1.1.215 | 0.065 | - |
Hydroxypyruvate | - |
Acetobacter pasteurianus | |
1.1.1.215 | 0.083 | - |
NADP+ | + D-gluconate | Acetobacter pasteurianus | |
1.1.1.215 | 0.11 | - |
NADPH | + 2-keto-L-gulonate | Acetobacter pasteurianus | |
1.1.1.215 | 0.14 | - |
NADPH | + hydroxypyruvate | Acetobacter pasteurianus | |
1.1.1.215 | 0.38 | - |
glyoxylate | - |
Acetobacter pasteurianus | |
1.1.1.215 | 0.71 | - |
NADPH | cosubstrate glyoxylate | Acetobacter pasteurianus | |
1.1.1.215 | 0.86 | - |
5-Keto-D-gluconate | - |
Acetobacter pasteurianus | |
1.1.1.215 | 2.4 | - |
D-gluconate | - |
Acetobacter pasteurianus | |
1.1.1.215 | 16 | - |
2-keto-D-galactonate | - |
Acetobacter pasteurianus | |
1.1.1.215 | 91 | - |
2-keto-L-gulonate | - |
Acetobacter pasteurianus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.215 | cytoplasm | - |
Acetobacter pasteurianus | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.215 | additional information | no metal requirement | Acetobacter pasteurianus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 15000 | - |
8 * 15000, SDS-PAGE | Acetobacter pasteurianus |
1.1.1.215 | 120000 | - |
gel filtration | Acetobacter pasteurianus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.215 | 2-dehydro-D-gluconate + NADPH | Acetobacter pasteurianus | - |
D-gluconate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.215 | Acetobacter pasteurianus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.215 | - |
Acetobacter pasteurianus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 336 | - |
- |
Acetobacter pasteurianus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.215 | -20°C, stable in the presence of sulfhydryl agents and ammonium sulfate | Acetobacter pasteurianus |
1.1.1.215 | 0°C, stable for at least a month in phosphate buffer | Acetobacter pasteurianus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.215 | 2-dehydro-D-gluconate + NADPH | - |
Acetobacter pasteurianus | D-gluconate + NADP+ | - |
r | |
1.1.1.215 | 2-keto-L-gulonate + NADPH | - |
Acetobacter pasteurianus | L-idonate + NADP+ | - |
r | |
1.1.1.215 | 5-keto-D-gluconate + NADPH | poor substrate | Acetobacter pasteurianus | D-gluconate + NADP+ | - |
? | |
1.1.1.215 | acetaldehyde + NADPH + H+ | poor substrate | Acetobacter pasteurianus | ethanol + NADP+ | - |
? | |
1.1.1.215 | D-galactonate + NADP+ | - |
Acetobacter pasteurianus | 2-dehydro-D-galactonate + NADPH | - |
r | |
1.1.1.215 | glyoxal + NADPH | reduction at 33% the rate of 2-ketogluconate reduction | Acetobacter pasteurianus | ? | - |
? | |
1.1.1.215 | glyoxylate + NADPH | reduction at 350% the rate of 2-ketogluconate reduction | Acetobacter pasteurianus | glycolate + NADP+ | - |
? | |
1.1.1.215 | hydroxypyruvate + NADPH | reduction at 733% the rate of 2-keto-gluconate reduction | Acetobacter pasteurianus | 2,3-dihydroxypropanoate + NADP+ | - |
? | |
1.1.1.215 | L-idonate + NADP+ | - |
Acetobacter pasteurianus | 2-keto-L-idonate + NADPH | - |
? | |
1.1.1.215 | pyruvate + NADPH + H+ | reduction at 7% the rate of 2-ketogluconate reduction | Acetobacter pasteurianus | lactate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.215 | octamer | 8 * 15000, SDS-PAGE | Acetobacter pasteurianus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 50 | - |
- |
Acetobacter pasteurianus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 40 | 55 | about half-maximal activity at 40°C and 55°C | Acetobacter pasteurianus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 55 | - |
stable for 10 min | Acetobacter pasteurianus |
1.1.1.215 | 60 | - |
rapid inactivation | Acetobacter pasteurianus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 7 | - |
reduction of 2-ketogluconate | Acetobacter pasteurianus |
1.1.1.215 | 12 | - |
- |
Acetobacter pasteurianus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 5 | 9.2 | about half-maximal activity at pH 5.0 and 9.2, reduction of ketogluconate | Acetobacter pasteurianus |
1.1.1.215 | 10.8 | 12.5 | about half-maximal activity at pH 10.8 and 12.5, oxidation of gluconate | Acetobacter pasteurianus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 6 | - |
stable for a week in the cold, increasing inactivation below and above, stable for a month or more with additional 2-mercaptoethanol | Acetobacter pasteurianus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.215 | NADP+ | - |
Acetobacter pasteurianus | |
1.1.1.215 | NADPH | - |
Acetobacter pasteurianus |