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Literature summary extracted from

  • Gross, W.; Seipold, P.; Schnarrenberger, C.
    Characterization and purification of an aldose from the acidophilic and thermophilic red algae Galdiera sulphuraria (1997), Plant Physiol., 114, 231-236.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 9.6
-
 D-fructose
-
 Galdieria sulphuraria
1.1.1.21 22
-
 L-sorbose
-
 Galdieria sulphuraria
1.1.1.21 24.8
-
 D-tagatose
-
 Galdieria sulphuraria
1.1.1.21 24.9
-
 D-xylose-5-phosphate
-
 Galdieria sulphuraria
1.1.1.21 27
-
 D-xylose
-
 Galdieria sulphuraria
1.1.1.21 29.1
-
 D-ribose-5-phosphate
-
 Galdieria sulphuraria
1.1.1.21 42.9
-
 D-galactose
-
 Galdieria sulphuraria
1.1.1.21 47.3
-
 D-glucose
-
 Galdieria sulphuraria
1.1.1.21 52.3
-
 L-rhamnose
-
 Galdieria sulphuraria
1.1.1.21 53.4
-
 D-mannose
-
 Galdieria sulphuraria
1.1.1.21 59.3
-
 D-fucose
-
 Galdieria sulphuraria
1.1.1.21 80.7
-
 L-fucose
-
 Galdieria sulphuraria
1.1.1.21 120.5
-
 hexanal
-
 Galdieria sulphuraria
1.1.1.21 245
-
 L-ribose
-
 Galdieria sulphuraria
1.1.1.21 312.3
-
 L-Lyxose
-
 Galdieria sulphuraria
1.1.1.21 370.9
-
 D-ribose
-
 Galdieria sulphuraria
1.1.1.21 416.8
-
 L-arabinose
-
 Galdieria sulphuraria
1.1.1.21 446.7
-
 D-arabinose
-
 Galdieria sulphuraria
1.1.1.21 459.2
-
 L-Xylose
-
 Galdieria sulphuraria
1.1.1.21 479.2
-
 D-Lyxose
-
 Galdieria sulphuraria
1.1.1.21 730.2
-
 DL-glyceraldehyde
-
 Galdieria sulphuraria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 40000
-
 2 * 40000, SDS-PAGE Galdieria sulphuraria
1.1.1.21 80000
-
 gel filtration Galdieria sulphuraria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.21 pentose + NADPH + H+ Galdieria sulphuraria with hexoses and sugar phosphates as substrate the activity is 5-10% of that with pentoses pentiol + NADP+
-
 r
1.1.1.21 pentose + NADPH + H+ Galdieria sulphuraria D-isoforms of pentoses show less than 10% activity compared to the L-isoforms pentiol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Galdieria sulphuraria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21
-
 Galdieria sulphuraria

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.21 17.1
-
 purified enzyme Galdieria sulphuraria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 D-arabinose + NADPH
-
 Galdieria sulphuraria arabitol + NADP+
-
 r
1.1.1.21 D-fructose + NAD(P)H + H+
-
 Galdieria sulphuraria D-arabino-hexitol + NAD(P)+
-
 r
1.1.1.21 D-fucose + NADPH
-
 Galdieria sulphuraria fucitol + NADP+
-
 r
1.1.1.21 D-galactose + NAD(P)H
-
 Galdieria sulphuraria dulcitol + NAD(P)+
-
 r
1.1.1.21 D-glucose + NAD(P)H
-
 Galdieria sulphuraria sorbitol + NAD(P)+
-
 r
1.1.1.21 D-lyxose + NADPH
-
 Galdieria sulphuraria arabitol + NADP+
-
 r
1.1.1.21 D-mannose + NAD(P)H
-
 Galdieria sulphuraria mannitol + NAD(P)+
-
 r
1.1.1.21 D-ribose + NAD(P)H
-
 Galdieria sulphuraria ribitol + NAD(P)+
-
 r
1.1.1.21 D-ribose + NADPH
-
 Galdieria sulphuraria adonitol + NADP+
-
 r
1.1.1.21 D-ribose 5-phosphate + NADPH
-
 Galdieria sulphuraria D-ribitol 5-phosphate + NADP+
-
 r
1.1.1.21 D-tagatose + NADPH
-
 Galdieria sulphuraria ?
-
 r
1.1.1.21 D-xylose + NAD(P)H
-
 Galdieria sulphuraria xylitol + NAD(P)+
-
 r
1.1.1.21 D-xylose 5-phosphate + NADPH
-
 Galdieria sulphuraria D-xylitol 5-phosphate + NADP+
-
 r
1.1.1.21 DL-glyceraldehyde + NAD(P)H
-
 Galdieria sulphuraria glycerol + NAD(P)+
-
 r
1.1.1.21 hexanal + NADPH + H+
-
 Galdieria sulphuraria hexanol + NADP+
-
 r
1.1.1.21 L-arabinose + NAD(P)H
-
 Galdieria sulphuraria L-arabitol + NAD(P)+
-
 r
1.1.1.21 L-arabinose + NADPH
-
 Galdieria sulphuraria arabitol + NADP+
-
 r
1.1.1.21 L-fucose + NADPH
-
 Galdieria sulphuraria fucitol + NADP+
-
 r
1.1.1.21 L-lyxose + NADPH
-
 Galdieria sulphuraria arabitol + NADP+
-
 r
1.1.1.21 L-rhamnose + NADPH
-
 Galdieria sulphuraria L-rhamnitol + NADP+
-
 r
1.1.1.21 L-ribose + NADPH
-
 Galdieria sulphuraria adonitol + NADP+
-
 r
1.1.1.21 L-sorbose + NADPH + H+
-
 Galdieria sulphuraria L-sorbitol + NADP+
-
 r
1.1.1.21 L-xylose + NAD(P)H
-
 Galdieria sulphuraria L-xylitol + NAD(P)+
-
 r
1.1.1.21 pentose + NADPH with hexoses and sugar phosphates as substrate the activity was only 5-10% of that with pentoses Galdieria sulphuraria pentitol + NADP+
-
 r
1.1.1.21 pentose + NADPH + H+ with hexoses and sugar phosphates as substrate the activity is 5-10% of that with pentoses Galdieria sulphuraria pentiol + NADP+
-
 r
1.1.1.21 pentose + NADPH + H+ D-isoforms of pentoses show less than 10% activity compared to the L-isoforms Galdieria sulphuraria pentiol + NADP+
-
 r
1.1.1.21 xylitol + NAD(P)+
-
 Galdieria sulphuraria D-xylose + NAD(P)H + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.21 dimer 2 * 40000, SDS-PAGE Galdieria sulphuraria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.21 6.8
-
-
 Galdieria sulphuraria

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.21 5.8 7.6 59% activity at both threshold values Galdieria sulphuraria

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADP+ NADP(H) dependent, no activity with NAD(H) in either directions Galdieria sulphuraria