EC Number | General Stability | Organism |
---|---|---|
1.1.1.20 | freezing and thawing: 3 times causes 25% decrease of activity, 5 times causes 40% decrease of activity | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.20 | Barbital | - |
Rattus norvegicus | |
1.1.1.20 | barbiturates | - |
Rattus norvegicus | |
1.1.1.20 | dithiothreitol | - |
Rattus norvegicus | |
1.1.1.20 | Hg2+ | - |
Rattus norvegicus | |
1.1.1.20 | iodoacetic acid | - |
Rattus norvegicus | |
1.1.1.20 | NaF | - |
Rattus norvegicus | |
1.1.1.20 | Phenobarbital | - |
Rattus norvegicus | |
1.1.1.20 | Zn2+ | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.20 | 0.045 | - |
NADPH | with D-glucuronic acid | Rattus norvegicus | |
1.1.1.20 | 4 | - |
D-galacturonic acid | - |
Rattus norvegicus | |
1.1.1.20 | 6 | - |
D-glucuronic acid | L-iduronic acid | Rattus norvegicus | |
1.1.1.20 | 9 | - |
D-glucuronolactone | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.20 | cytoplasm | - |
Rattus norvegicus | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.20 | 40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
1.1.1.20 | 41000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.20 | L-gulono-1,4-lactone + NADP+ | Rattus norvegicus | - |
D-glucurono-3,6-lactone + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.20 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.20 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.20 | kidney | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.20 | additional information | - |
13450 U/min * mg, 1 unit is defined as change of absorbance of 0.01/min at 340 nm | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.20 | -80°C, 10 days, 30% loss of activity | Rattus norvegicus |
1.1.1.20 | 0°C, 2 months, less than 10% loss of activity | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.20 | D-galacturonic acid + NADPH | - |
Rattus norvegicus | ? | - |
? | |
1.1.1.20 | D-glucuronic acid + NADPH | - |
Rattus norvegicus | L-gulonic acid + NADP+ | - |
? | |
1.1.1.20 | D-glucurono-3,6-lactone + NADPH | oxidation activity is very low | Rattus norvegicus | D-gulono-1,4-lactone + NADP+ | - |
r | |
1.1.1.20 | D-iduronic acid + NADPH | - |
Rattus norvegicus | ? | - |
? | |
1.1.1.20 | erythrose + NADPH | - |
Rattus norvegicus | glycerol + NADP+ | - |
? | |
1.1.1.20 | glyceraldehyde + NADPH | - |
Rattus norvegicus | ? | - |
? | |
1.1.1.20 | L-gulono-1,4-lactone + NADP+ | - |
Rattus norvegicus | D-glucurono-3,6-lactone + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.20 | monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.20 | 40 | - |
30 min, 20% loss of activity | Rattus norvegicus |
1.1.1.20 | 40 | - |
60 min, 30% loss of activity | Rattus norvegicus |
1.1.1.20 | 40 | - |
180 min, 50% loss of activity | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.20 | 5.5 | 7.5 | DL-glyceraldehyde | Rattus norvegicus |
1.1.1.20 | 6 | 7 | D-glucuronic acid | Rattus norvegicus |
1.1.1.20 | 9.5 | - |
broad, oxidation of glycerol and L-gulonolactone | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.20 | NADH | NADPH is 145-times as effective as NADH | Rattus norvegicus | |
1.1.1.20 | NADP+ | - |
Rattus norvegicus | |
1.1.1.20 | NADPH | NADPH is 145-times as effective as NADH | Rattus norvegicus |