EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.2 | ADH gene cloned and overexpressed in Escherichia coli | Thermococcus zilligii |
1.1.1.2 | gene encoding NADPH group III alcohol dehydrogenase cloned and overexpressed in Escherichia coli | Thermococcus hydrothermalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.2 | 0.01 | - |
benzaldehyde | co-substrate NADP | Thermococcus hydrothermalis | |
1.1.1.2 | 0.13 | - |
NADPH | benzaldehyde as substrate | Thermococcus hydrothermalis | |
1.1.1.2 | 0.8 | - |
NADP+ | benzyl aclohol as substrate | Thermococcus hydrothermalis | |
1.1.1.2 | 2 | - |
benzyl alcohol | co-substrate NADP | Thermococcus hydrothermalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 45000 | - |
4 * 45000, pH 10.5 | Thermococcus hydrothermalis |
1.1.1.2 | 80500 | - |
pH-dependent enzyme structure, pH 7.5 | Thermococcus hydrothermalis |
1.1.1.2 | 197000 | - |
pH-dependent enzyme structure, pH 10.5 | Thermococcus hydrothermalis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.2 | Aquifex aeolicus | - |
- |
- |
1.1.1.2 | Archaeoglobus fulgidus | - |
- |
- |
1.1.1.2 | Bacillus methanolicus | - |
- |
- |
1.1.1.2 | Clostridium acetobutylicum | - |
- |
- |
1.1.1.2 | Pyrococcus furiosus | - |
- |
- |
1.1.1.2 | Saccharomyces cerevisiae | - |
yeast | - |
1.1.1.2 | Schizosaccharomyces pombe | - |
- |
- |
1.1.1.2 | Thermococcus hydrothermalis | - |
- |
- |
1.1.1.2 | Thermococcus litoralis | - |
- |
- |
1.1.1.2 | Thermococcus zilligii | - |
- |
- |
1.1.1.2 | Zymomonas mobilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.2 | - |
Thermococcus hydrothermalis |
1.1.1.2 | - |
Thermococcus zilligii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 6.66 | - |
native enzyme | Thermococcus hydrothermalis |
1.1.1.2 | 24 | - |
recombinant enzyme | Thermococcus hydrothermalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.2 | 1,3-propanediol + NADP+ | - |
Archaeoglobus fulgidus | ? + NADPH | - |
? | |
1.1.1.2 | 1,3-propanediol + NADP+ | - |
Thermococcus litoralis | ? + NADPH | - |
? | |
1.1.1.2 | benzaldehyde + NADPH + H+ | - |
Thermococcus hydrothermalis | benzyl alcohol + NADP+ | - |
r | |
1.1.1.2 | benzyl alcohol + NADP+ | - |
Thermococcus hydrothermalis | benzaldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | glycerol + NADP+ | - |
Thermococcus hydrothermalis | DL-glyceraldehyde + NADPH + H+ | - |
r | |
1.1.1.2 | methanol + NADP+ | - |
Thermococcus hydrothermalis | formaldehyde + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.2 | dimer | - |
Saccharolobus solfataricus |
1.1.1.2 | dimer | - |
Thermococcus hydrothermalis |
1.1.1.2 | tetramer | - |
Thermococcus litoralis |
1.1.1.2 | tetramer | 4 * 45000, pH 10.5 | Thermococcus hydrothermalis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.2 | putative iron alcohol dehydrogenase | - |
Schizosaccharomyces pombe |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.2 | 7.5 | - |
optimum for aldehyde reduction | Thermococcus hydrothermalis |
1.1.1.2 | 10.5 | - |
optimum for alcohol oxidation | Thermococcus hydrothermalis |