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Literature summary extracted from

  • Antoine, E.; Rolland, J.L.; Raffin, J.P.; Dietrich, J.
    Cloning and over-expression in Escherichia coli of the gene encoding NADPH group III alcohol dehydrogenase from Thermococcus hydrothermalis. Characterization and comparison of the native and the recombinant enzymes (1999), Eur. J. Biochem., 264, 880-889.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 ADH gene cloned and overexpressed in Escherichia coli Thermococcus zilligii
1.1.1.2 gene encoding NADPH group III alcohol dehydrogenase cloned and overexpressed in Escherichia coli Thermococcus hydrothermalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.01
-
benzaldehyde co-substrate NADP Thermococcus hydrothermalis
1.1.1.2 0.13
-
NADPH benzaldehyde as substrate Thermococcus hydrothermalis
1.1.1.2 0.8
-
NADP+ benzyl aclohol as substrate Thermococcus hydrothermalis
1.1.1.2 2
-
benzyl alcohol co-substrate NADP Thermococcus hydrothermalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 45000
-
4 * 45000, pH 10.5 Thermococcus hydrothermalis
1.1.1.2 80500
-
pH-dependent enzyme structure, pH 7.5 Thermococcus hydrothermalis
1.1.1.2 197000
-
pH-dependent enzyme structure, pH 10.5 Thermococcus hydrothermalis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Aquifex aeolicus
-
-
-
1.1.1.2 Archaeoglobus fulgidus
-
-
-
1.1.1.2 Bacillus methanolicus
-
-
-
1.1.1.2 Clostridium acetobutylicum
-
-
-
1.1.1.2 Pyrococcus furiosus
-
-
-
1.1.1.2 Saccharomyces cerevisiae
-
yeast
-
1.1.1.2 Schizosaccharomyces pombe
-
-
-
1.1.1.2 Thermococcus hydrothermalis
-
-
-
1.1.1.2 Thermococcus litoralis
-
-
-
1.1.1.2 Thermococcus zilligii
-
-
-
1.1.1.2 Zymomonas mobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
Thermococcus hydrothermalis
1.1.1.2
-
Thermococcus zilligii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 6.66
-
native enzyme Thermococcus hydrothermalis
1.1.1.2 24
-
recombinant enzyme Thermococcus hydrothermalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 1,3-propanediol + NADP+
-
Archaeoglobus fulgidus ? + NADPH
-
?
1.1.1.2 1,3-propanediol + NADP+
-
Thermococcus litoralis ? + NADPH
-
?
1.1.1.2 benzaldehyde + NADPH + H+
-
Thermococcus hydrothermalis benzyl alcohol + NADP+
-
r
1.1.1.2 benzyl alcohol + NADP+
-
Thermococcus hydrothermalis benzaldehyde + NADPH + H+
-
r
1.1.1.2 glycerol + NADP+
-
Thermococcus hydrothermalis DL-glyceraldehyde + NADPH + H+
-
r
1.1.1.2 methanol + NADP+
-
Thermococcus hydrothermalis formaldehyde + NADPH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 dimer
-
Saccharolobus solfataricus
1.1.1.2 dimer
-
Thermococcus hydrothermalis
1.1.1.2 tetramer
-
Thermococcus litoralis
1.1.1.2 tetramer 4 * 45000, pH 10.5 Thermococcus hydrothermalis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 putative iron alcohol dehydrogenase
-
Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7.5
-
optimum for aldehyde reduction Thermococcus hydrothermalis
1.1.1.2 10.5
-
optimum for alcohol oxidation Thermococcus hydrothermalis