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Literature summary extracted from

  • Colrat, S.; Latche, A.; Guis, M.; Pech, J.C.; Bouzayen, M.; Fallot, J.; Roustan, J.P.
    Purification and characterization of a NADPH-dependent aldehyde reductase from mung bean that detoxifies eutypine, a toxin from Eutypa lata1 (1999), Plant Physiol., 119, 621-626.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.2 agriculture detoxification of eutypine toxin from Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera Vigna radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.0029
-
 m-nitrobenzaldehyde
-
 Vigna radiata
1.1.1.2 0.0033
-
 decanal
-
 Vigna radiata
1.1.1.2 0.0045
-
 4-pyridine carboxaldehyde
-
 Vigna radiata
1.1.1.2 0.0053
-
 4-nitrobenzaldehyde
-
 Vigna radiata
1.1.1.2 0.0054
-
 3-Fluorobenzaldehyde
-
 Vigna radiata
1.1.1.2 0.0063
-
 (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) eutypine Vigna radiata
1.1.1.2 0.015
-
 1-hexanal
-
 Vigna radiata
1.1.1.2 0.02
-
 m-methylbenzaldehyde
-
 Vigna radiata
1.1.1.2 0.033
-
 benzaldehyde
-
 Vigna radiata
1.1.1.2 0.04
-
 p-fluorobenzaldehyde
-
 Vigna radiata
1.1.1.2 0.041
-
 p-methylbenzaldehyde
-
 Vigna radiata
1.1.1.2 0.049
-
 2-Methoxybenzaldehyde
-
 Vigna radiata
1.1.1.2 0.06
-
 coniferylaldehyde
-
 Vigna radiata
1.1.1.2 0.066
-
 Salicylaldehyde
-
 Vigna radiata
1.1.1.2 0.087
-
 propionaldehyde
-
 Vigna radiata
1.1.1.2 0.099
-
 cinnamaldehyde
-
 Vigna radiata
1.1.1.2 0.125
-
 o-methylbenzaldehyde
-
 Vigna radiata
1.1.1.2 0.126
-
 sinapylaldehyde
-
 Vigna radiata
1.1.1.2 0.15
-
 p-Methoxybenzaldehyde
-
 Vigna radiata
1.1.1.2 0.207
-
 furaldehyde
-
 Vigna radiata
1.1.1.2 0.212
-
 m-methoxybenzaldehyde
-
 Vigna radiata
1.1.1.2 0.376
-
 o-nitrobenzaldehyde
-
 Vigna radiata
1.1.1.2 0.702
-
 o-fluorobenzaldehyde
-
 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 36000
-
-
 Vigna radiata
1.1.1.2 36000
-
 1 * 36000 Thermococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.2 (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+ Vigna radiata eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+ eutypinol, non-toxic metabolite ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Thermococcus sp.
-
-
-
1.1.1.2 Vigna radiata
-
 mung bean
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 additional information
-
 891 nkat/mg Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzaldehyde) + NADPH + H+ eutypine, a toxin produced by Eutypa lata, the causal agent of Eutypa dieback in the grapevine Vitis vinifera Vigna radiata (4-hydroxy-3-[3-methyl-3-butene-1-ynyl]benzyl alcohol) + NADP+ eutypinol, non-toxic metabolite ir
1.1.1.2 2-methoxybenzaldehyde + NADPH + H+
-
 Vigna radiata 2-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.2 4-pyridinecarboxaldehyde + NADPH
-
 Vigna radiata 4-pyridinemethanol + NADP+
-
 ?
1.1.1.2 benzaldehyde + NADPH
-
 Vigna radiata benzyl alcohol + NADP+
-
 ?
1.1.1.2 coniferaldehyde + NADPH converted less efficiently than eutypine Vigna radiata coniferyl alcohol + NADP+
-
 ?
1.1.1.2 furaldehyde + NADPH
-
 Vigna radiata furfuryl alcohol + NADP+
-
 ?
1.1.1.2 hexanal + NADPH + H+
-
 Vigna radiata hexanol + NADP+
-
 ?
1.1.1.2 m-fluorobenzaldehyde + NADPH + H+
-
 Vigna radiata m-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.2 m-methoxybenzaldehyde + NADPH + H+
-
 Vigna radiata m-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.2 m-methylbenzaldehyde + NADPH
-
 Vigna radiata m-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.2 m-nitrobenzaldehyde + NADPH
-
 Vigna radiata m-nitrobenzylalcohol + NADP+
-
 ?
1.1.1.2 additional information
-
 Thermococcus sp. ?
-
 ?
1.1.1.2 additional information dihydroquercitin and dihydromyricetin are no substrates Vigna radiata ?
-
 ?
1.1.1.2 n-decanal + NADPH + H+ converted less efficiently than eutypine Vigna radiata n-decanol + NADP+
-
 ?
1.1.1.2 o-fluorobenzaldehyde + NADPH + H+
-
 Vigna radiata o-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.2 o-methylbenzaldehyde + NADPH
-
 Vigna radiata o-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.2 o-nitrobenzaldehyde + NADPH
-
 Vigna radiata o-nitrobenzylalcohol + NADP+
-
 ?
1.1.1.2 p-fluorobenzaldehyde + NADPH + H+
-
 Vigna radiata p-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.2 p-methoxybenzaldehyde + NADPH + H+
-
 Vigna radiata p-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.2 p-methylbenzaldehyde + NADPH
-
 Vigna radiata p-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.2 p-nitrobenzaldehyde + NADPH
-
 Vigna radiata p-nitrobenzylalcohol + NADP+
-
 ?
1.1.1.2 propionaldehyde + NADPH
-
 Vigna radiata propanol + NADP+
-
 ?
1.1.1.2 salicylaldehyde + NADPH
-
 Vigna radiata 2-hydroxymethylphenol + NADP+
-
 ?
1.1.1.2 sinapaldehyde + NADPH + H+ converted less efficiently than eutypine Vigna radiata sinapyl alcohol + NADP+
-
 ?
1.1.1.2 trans-cinnamaldehyde + NADPH converted less efficiently than eutypine Vigna radiata cinnamyl alcohol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 monomer
-
 Vigna radiata
1.1.1.2 monomer 1 * 36000 Thermococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.2 6.2 7.5
-
 Vigna radiata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH
-
 Vigna radiata
1.1.1.2 NADPH
-
 Thermococcus sp.