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Literature summary extracted from

  • Wermuth, B.; Muench, J.D.B.; von Wartburg, J.P.
    Purification and properties of NADPH-dependent aldehyde reductase from human liver (1977), J. Biol. Chem., 252, 3821-3828.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.2 presence of NADPH or NADP+ prevents loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 ethylphenylhydantoin
-
 Homo sapiens
1.1.1.2 Phenobarbital
-
 Homo sapiens
1.1.1.2 Phenobarbital
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.2 0.025
-
 p-Carboxybenzaldehyde
-
 Homo sapiens
1.1.1.2 0.15
-
 p-nitrobenzaldehyde
-
 Homo sapiens
1.1.1.2 0.94
-
 p-chlorobenzaldehyde
-
 Homo sapiens
1.1.1.2 1.3
-
 methylglyoxal
-
 Homo sapiens
1.1.1.2 1.3
-
 Butyraldehyde
-
 Homo sapiens
1.1.1.2 1.4
-
 Camphorquinone
-
 Homo sapiens
1.1.1.2 1.5
-
 DL-glyceraldehyde
-
 Homo sapiens
1.1.1.2 2.4
-
 m-nitrobenzaldehyde
-
 Homo sapiens
1.1.1.2 2.9
-
 propionaldehyde
-
 Homo sapiens
1.1.1.2 3.4
-
 benzaldehyde
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 30000
-
-
 Sus scrofa
1.1.1.2 30000
-
 gel filtration Homo sapiens
1.1.1.2 33600
-
 sedimentation analysis Homo sapiens
1.1.1.2 36200
-
 gel filtration Homo sapiens
1.1.1.2 36300
-
 ultracentrifugation Homo sapiens
1.1.1.2 39400
-
 1 * 39400, SDS-PAGE Homo sapiens
1.1.1.2 44000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.2 D-glyceraldehyde + NADPH + H+ Homo sapiens
-
 glycerol + NADP+
-
 r
1.1.1.2 D-glyceraldehyde + NADPH + H+ Sus scrofa
-
 glycerol + NADP+
-
 r
1.1.1.2 L-glyceraldehyde + NADPH Homo sapiens
-
 glycerol + NADP+
-
 r
1.1.1.2 L-glyceraldehyde + NADPH Sus scrofa
-
 glycerol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Equus sp.
-
 horse
-
1.1.1.2 Homo sapiens
-
 human
-
1.1.1.2 Pecten maximus
-
 mollusc
-
1.1.1.2 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.2 brain
-
 Homo sapiens
-
1.1.1.2 brain
-
 Sus scrofa
-
1.1.1.2 heart
-
 Homo sapiens
-
1.1.1.2 heart
-
 Sus scrofa
-
1.1.1.2 kidney
-
 Homo sapiens
-
1.1.1.2 kidney
-
 Sus scrofa
-
1.1.1.2 lens
-
 Homo sapiens
-
1.1.1.2 lens
-
 Sus scrofa
-
1.1.1.2 liver
-
 Homo sapiens
-
1.1.1.2 liver
-
 Sus scrofa
-
1.1.1.2 liver
-
 Equus sp.
-
1.1.1.2 muscle
-
 Sus scrofa
-
1.1.1.2 muscle
-
 Pecten maximus
-
1.1.1.2 muscle skeletal muscle Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 14.3
-
 pure aldehyde reductase Homo sapiens
1.1.1.2 15.7
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.2 4°C stable for several months but loss of activity with a concomitant enzyme precipitation occurs upon freezing and thawing Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 (+)-camphorquinone + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.2 (+)-camphorquinone + NADPH
-
 Sus scrofa ? + NADP+
-
 ?
1.1.1.2 4-hydroxy-3-methoxybenzyl alcohol + NADP+
-
 Homo sapiens 4-hydroxy-3-methoxybenzaldehyde + NADPH
-
 r
1.1.1.2 benzaldehyde + NADPH
-
 Homo sapiens benzyl alcohol + NADP+
-
 r
1.1.1.2 benzaldehyde + NADPH
-
 Sus scrofa benzyl alcohol + NADP+
-
 r
1.1.1.2 camphorquinone + NADPH + H+
-
 Homo sapiens 1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+
-
 ?
1.1.1.2 D-glyceraldehyde + NADPH + H+
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.2 D-glyceraldehyde + NADPH + H+
-
 Sus scrofa glycerol + NADP+
-
 r
1.1.1.2 D-lactaldehyde + NADPH + H+
-
 Homo sapiens propane-1,2-diol + NADP+
-
 r
1.1.1.2 D-lactaldehyde + NADPH + H+
-
 Sus scrofa propane-1,2-diol + NADP+
-
 r
1.1.1.2 DL-glyceraldehyde + NADPH + H+
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.2 L-glyceraldehyde + NADPH
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.2 L-glyceraldehyde + NADPH
-
 Sus scrofa glycerol + NADP+
-
 r
1.1.1.2 m-nitrobenzaldehyde + NADPH
-
 Homo sapiens m-nitrobenzylalcohol + NADP+
-
 r
1.1.1.2 m-nitrobenzaldehyde + NADPH
-
 Sus scrofa m-nitrobenzylalcohol + NADP+
-
 r
1.1.1.2 methylglyoxal + NADPH
-
 Homo sapiens ? + NADP+
-
 r
1.1.1.2 methylglyoxal + NADPH
-
 Sus scrofa ? + NADP+
-
 r
1.1.1.2 additional information
-
 Sus scrofa ?
-
 ?
1.1.1.2 additional information no enzymatic activity in the presence of NADPH with acetaldehyde, pyridoxal, pyridoxal phosphate, glyceraldehyde phosphate, glucose or fructose, no activity in the reverse reaction with ethanol, cyclohexanol, phenylpropyl alcohol and glycerol phosphate Homo sapiens ?
-
 ?
1.1.1.2 n-butyraldehyde + NADPH
-
 Homo sapiens n-butanol + NADP+
-
 r
1.1.1.2 n-butyraldehyde + NADPH
-
 Sus scrofa n-butanol + NADP+
-
 r
1.1.1.2 p-carboxybenzaldehyde + NADPH
-
 Homo sapiens p-carboxybenzyl alcohol + NADP+
-
 r
1.1.1.2 p-carboxybenzaldehyde + NADPH
-
 Sus scrofa p-carboxybenzyl alcohol + NADP+
-
 r
1.1.1.2 p-chlorobenzaldehyde + NADPH + H+
-
 Homo sapiens p-chlorobenzyl alcohol + NADP+
-
 r
1.1.1.2 p-chlorobenzaldehyde + NADPH + H+
-
 Sus scrofa p-chlorobenzyl alcohol + NADP+
-
 r
1.1.1.2 p-nitrobenzaldehyde + NADPH cofactor NADPH Homo sapiens p-nitrobenzylalcohol + NADP+
-
 r
1.1.1.2 p-nitrobenzaldehyde + NADPH cofactor NADPH Sus scrofa p-nitrobenzylalcohol + NADP+
-
 r
1.1.1.2 phenylethanediol + NADP+
-
 Homo sapiens ?
-
 r
1.1.1.2 phenylethanol + NADP+
-
 Homo sapiens phenylacetaldehyde + NADPH + H+
-
 r
1.1.1.2 propane-1,2-diol + NADP+
-
 Homo sapiens lactaldehyde + NADPH
-
 r
1.1.1.2 propane-1,2-diol + NADP+
-
 Sus scrofa lactaldehyde + NADPH
-
 r
1.1.1.2 propionaldehyde + NADPH
-
 Homo sapiens propanol + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.2 monomer
-
 Sus scrofa
1.1.1.2 monomer
-
 Pecten maximus
1.1.1.2 monomer 1 * 39400, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 liver alcohol dehydrogenase
-
 Equus sp.
1.1.1.2 Octopine dehydrogenase
-
 Pecten maximus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.2 0.0283
-
 glycerol
-
 Homo sapiens
1.1.1.2 0.0283
-
 Phenylethanol
-
 Homo sapiens
1.1.1.2 0.0433
-
 Propane-1,2-diol
-
 Homo sapiens
1.1.1.2 0.0733
-
 benzylalcohol
-
 Homo sapiens
1.1.1.2 0.08
-
 phenylethanediol
-
 Homo sapiens
1.1.1.2 0.148
-
 4-Hydroxy-3-methoxybenzyl alcohol
-
 Homo sapiens
1.1.1.2 9.5
-
 p-nitrobenzaldehyde
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 6.6
-
 reduction of p-nitrobenzaldehyde in presence of NADPH Homo sapiens
1.1.1.2 9 9.5 oxidation of benzyl alcohol in presence of NADP+ Homo sapiens