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Literature summary extracted from

  • Ludwig-Muller, J.; Schramm, P.; Hilgenberg, W.
    Indole-3-acetaldehyde reductase in Phycomyces blakesleeanus. Characterization of the enzyme (1990), Physiol. Plant., 80, 472-478.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.191 hydroxymethylindole 42% activation at 0.2 mM Phycomyces blakesleeanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.191 Cu2+ 59% inhibition at 1 mM Phycomyces blakesleeanus
1.1.1.191 Hg2+ 50% inhibition at 0.01 mM Phycomyces blakesleeanus
1.1.1.191 Indole-3-aldehyde slight inhibition at 0.2 mM Phycomyces blakesleeanus
1.1.1.191 indole-3-glycolate 62% inhibition at 0.2 mM Phycomyces blakesleeanus
1.1.1.191 KI 23% inhibition at 1 mM Phycomyces blakesleeanus
1.1.1.191 Mn2+ 46% inhibition at 1 mM Phycomyces blakesleeanus
1.1.1.191 NADP+ non-competitive inhibition, half-maximal inhibition at 0.2 mM Phycomyces blakesleeanus
1.1.1.191 Zn2+ 36% inhibition at 1 mM Phycomyces blakesleeanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.191 0.024
-
 NADPH
-
 Phycomyces blakesleeanus
1.1.1.191 0.2
-
 Indole-3-acetaldehyde
-
 Phycomyces blakesleeanus
1.1.1.191 0.645
-
 dihydroxyacetone phosphate
-
 Phycomyces blakesleeanus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.191 Fe3+ 22% activation at 0.1 mM Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.191 indole-3-acetaldehyde + NADPH Phycomyces blakesleeanus
-
 indole-3-ethanol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.191 Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.191 partial Phycomyces blakesleeanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.191 cell culture maximum enzyme activity from cultures after 48 h Phycomyces blakesleeanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.191 0.06
-
 light-grown cells Phycomyces blakesleeanus
1.1.1.191 0.11
-
 dark-grown cells Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.191 acetaldehyde + NADPH + H+ very low activity Phycomyces blakesleeanus ethanol + NADP+
-
 ?
1.1.1.191 benzaldehyde + NADPH 2.5fold higher activity than for indole-3-acetaldehyde Phycomyces blakesleeanus benzyl alcohol + NADP+
-
 ?
1.1.1.191 dihydroxyacetonephosphate + NADPH well accepted substrate Phycomyces blakesleeanus glycerol 1-phosphate + NADP+
-
 ?
1.1.1.191 indole-3-acetaldehyde + NADPH
-
 Phycomyces blakesleeanus indole-3-ethanol + NADP+
-
 ?
1.1.1.191 indole-3-aldehyde + NADPH 19% of the activity of indole-3-acetaldehyde Phycomyces blakesleeanus indole-3-ol + NADP+
-
 ?
1.1.1.191 phenylacetaldehyde + NADPH + H+ well accepted substrate Phycomyces blakesleeanus phenylethanol + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.191 30
-
-
 Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.191 6 8
-
 Phycomyces blakesleeanus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.191 NADP+ with NAD+ 10times lower activity Phycomyces blakesleeanus