Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rahimtula, A.D.; Gaylor, J.L.
    Investigation of the component reactions of oxidative sterol demethylation. Partial purification of a microsomal sterol 4alpha-carboxylic acid decarboxylase (1972), J. Biol. Chem., 247, 9-15.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.170 5alpha-cholest-7-en-3-one 2-9.3% inhibition between 16 and 130 nmol Rattus sp.
1.1.1.170 5alpha-Cholest-7-en-3beta-ol 15.5-65% inhibition between 30 and 260 nmol Rattus sp.
1.1.1.170 Fe2+ less than 25% inhibition at 1 mM Rattus sp.
1.1.1.170 Zn2+ marked inhibition between 0.1 and 1 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.170 0.007
-
 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid
-
 Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.170 3beta-hydroxy-4alpha-methyl-5alpha-cholest-7-ene-4beta-carboxylate + NAD+ Rattus sp.
-
 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
 ?
1.1.1.170 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid + NAD+ Rattus sp. no epimerization of 4beta-isomer into 4alpha-isomer 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.170 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.170 phospholipoprotein presence of phosphatidylcholine and phosphatidylethanolamine, no loss of activity after removal of phospholipids Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.170 near homogeneity, chromatography techniques, sodium deoxycholate solubilization Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.170 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.170 0.11
-
-
 Rattus sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.170 4°C, 10 mM phosphate buffer, pH 7.4, no loss of activity within 2 weeks Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.170 3beta-hydroxy-4alpha-methyl-5alpha-cholest-7-ene-4beta-carboxylate + NAD+
-
 Rattus sp. 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
 ?
1.1.1.170 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-en-4alpha-oic acid + NAD+ no epimerization of 4beta-isomer into 4alpha-isomer Rattus sp. 4alpha-methyl-5alpha-cholest-7-en-3-one + CO2 + NADH
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.1.1.170 4alpha-carboxylic acid decarboxylase
-
 Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.170 8 9
-
 Rattus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.170 additional information
-
 acidic pH results in a very low decarboxylation rate Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.170 NAD+ can not be replaced by NADP+ Rattus sp.