EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.169 | expression in Escherichia coli BL21/lambdaDE3 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.169 | 0.742 | - |
ketopantoic acid | +/-0.01 | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.169 | 0.776 | - |
NADPH | +/-0.09 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 31100 | - |
HPLC gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | ketopantoate + NADPH | Escherichia coli | - |
pantoate + NADP+ | - |
? | |
1.1.1.169 | ketopantoate + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | thiamine synthesis, pantothenate and thiamine biosynthetic pathway | pantoate + NADP+ | - |
? | |
1.1.1.169 | ketopantoate + NADPH | Escherichia coli BL21/lambdaDE3 | - |
pantoate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.169 | Escherichia coli | - |
BL21/lambdaDE3 | - |
1.1.1.169 | Escherichia coli BL21/lambdaDE3 | - |
BL21/lambdaDE3 | - |
1.1.1.169 | Saccharomyces cerevisiae | - |
- |
- |
1.1.1.169 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
1.1.1.169 | Stenotrophomonas maltophilia | - |
845 | - |
1.1.1.169 | Stenotrophomonas maltophilia 845 | - |
845 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.169 | chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | additional information | - |
- |
Escherichia coli |
1.1.1.169 | additional information | - |
- |
Saccharomyces cerevisiae |
1.1.1.169 | additional information | - |
no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
1.1.1.169 | 16 | - |
optimal reaction conditions using NADPH | Salmonella enterica subsp. enterica serovar Typhimurium |
1.1.1.169 | 61 | - |
optimal reaction conditions using NADH | Salmonella enterica subsp. enterica serovar Typhimurium |
1.1.1.169 | 64.3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | ketopantoate + NADPH | - |
Escherichia coli | pantoate + NADP+ | - |
? | |
1.1.1.169 | ketopantoate + NADPH | thiamine synthesis, pantothenate and thiamine biosynthetic pathway | Salmonella enterica subsp. enterica serovar Typhimurium | pantoate + NADP+ | - |
? | |
1.1.1.169 | ketopantoate + NADPH | - |
Escherichia coli BL21/lambdaDE3 | pantoate + NADP+ | - |
? | |
1.1.1.169 | ketopantoic acid + NADH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pantoic acid + NAD+ | - |
? | |
1.1.1.169 | ketopantoic acid + NADPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pantoic acid + NADP+ | - |
? | |
1.1.1.169 | ketopantoic acid + NADPH | - |
Escherichia coli | pantoic acid + NADP+ | - |
? | |
1.1.1.169 | ketopantoic acid + NADPH | - |
Saccharomyces cerevisiae | pantoic acid + NADP+ | - |
? | |
1.1.1.169 | ketopantoic acid + NADPH | - |
Escherichia coli BL21/lambdaDE3 | pantoic acid + NADP+ | - |
? | |
1.1.1.169 | additional information | - |
Escherichia coli | ? | - |
? | |
1.1.1.169 | additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.169 | additional information | no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
1.1.1.169 | additional information | - |
Escherichia coli BL21/lambdaDE3 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.169 | monomer | - |
Escherichia coli |
1.1.1.169 | monomer | 1 * 31100, HPLC gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 42 | - |
reduction of ketopantoic acid at pH 6.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 6.3 | - |
reduction of pantoic acid at 42°C | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | additional information | - |
Stenotrophomonas maltophilia | |
1.1.1.169 | additional information | in presence of NADPH the ketopantoic acid reductase does not reduce artificial dye electron acceptors | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.169 | NADH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.1.1.169 | NADH | - |
Saccharomyces cerevisiae | |
1.1.1.169 | NADPH | - |
Escherichia coli | |
1.1.1.169 | NADPH | - |
Saccharomyces cerevisiae | |
1.1.1.169 | NADPH | - |
Stenotrophomonas maltophilia | |
1.1.1.169 | NADPH | preferred | Salmonella enterica subsp. enterica serovar Typhimurium |