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Literature summary extracted from

  • Frodyma, M.E.; Downs, D.
    ApbA, the ketopantoate reductase enzyme of Salmonella typhimurium is required for the synthesis of thiamine via the alternative pyrimidine biosynthetic pathway (1998), J. Biol. Chem., 273, 5572-5576.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.169 expression in Escherichia coli BL21/lambdaDE3 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.169 0.742
-
 ketopantoic acid +/-0.01 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 0.776
-
 NADPH +/-0.09 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.169 31100
-
 HPLC gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.169 ketopantoate + NADPH Escherichia coli
-
 pantoate + NADP+
-
 ?
1.1.1.169 ketopantoate + NADPH Salmonella enterica subsp. enterica serovar Typhimurium thiamine synthesis, pantothenate and thiamine biosynthetic pathway pantoate + NADP+
-
 ?
1.1.1.169 ketopantoate + NADPH Escherichia coli BL21/lambdaDE3
-
 pantoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.169 Escherichia coli
-
 BL21/lambdaDE3
-
1.1.1.169 Escherichia coli BL21/lambdaDE3
-
 BL21/lambdaDE3
-
1.1.1.169 Saccharomyces cerevisiae
-
-
-
1.1.1.169 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
1.1.1.169 Stenotrophomonas maltophilia
-
 845
-
1.1.1.169 Stenotrophomonas maltophilia 845
-
 845
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.169 chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.169 additional information
-
-
 Escherichia coli
1.1.1.169 additional information
-
-
 Saccharomyces cerevisiae
1.1.1.169 additional information
-
 no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 16
-
 optimal reaction conditions using NADPH Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 61
-
 optimal reaction conditions using NADH Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 64.3
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.169 ketopantoate + NADPH
-
 Escherichia coli pantoate + NADP+
-
 ?
1.1.1.169 ketopantoate + NADPH thiamine synthesis, pantothenate and thiamine biosynthetic pathway Salmonella enterica subsp. enterica serovar Typhimurium pantoate + NADP+
-
 ?
1.1.1.169 ketopantoate + NADPH
-
 Escherichia coli BL21/lambdaDE3 pantoate + NADP+
-
 ?
1.1.1.169 ketopantoic acid + NADH
-
 Salmonella enterica subsp. enterica serovar Typhimurium pantoic acid + NAD+
-
 ?
1.1.1.169 ketopantoic acid + NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium pantoic acid + NADP+
-
 ?
1.1.1.169 ketopantoic acid + NADPH
-
 Escherichia coli pantoic acid + NADP+
-
 ?
1.1.1.169 ketopantoic acid + NADPH
-
 Saccharomyces cerevisiae pantoic acid + NADP+
-
 ?
1.1.1.169 ketopantoic acid + NADPH
-
 Escherichia coli BL21/lambdaDE3 pantoic acid + NADP+
-
 ?
1.1.1.169 additional information
-
 Escherichia coli ?
-
 ?
1.1.1.169 additional information
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.169 additional information no activity with pantoate, ketoisovalerate, oxaloacetate, pyruvate, 3-hydroxypyruvic acid, alpha-ketoglutarate, alpha-ketobutyrate, acetaldehyde at 0.5 mM Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
1.1.1.169 additional information
-
 Escherichia coli BL21/lambdaDE3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.169 monomer
-
 Escherichia coli
1.1.1.169 monomer 1 * 31100, HPLC gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.169 42
-
 reduction of ketopantoic acid at pH 6.0 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.169 6.3
-
 reduction of pantoic acid at 42°C Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.169 additional information
-
 Stenotrophomonas maltophilia
1.1.1.169 additional information in presence of NADPH the ketopantoic acid reductase does not reduce artificial dye electron acceptors Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 NADH
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.169 NADH
-
 Saccharomyces cerevisiae
1.1.1.169 NADPH
-
 Escherichia coli
1.1.1.169 NADPH
-
 Saccharomyces cerevisiae
1.1.1.169 NADPH
-
 Stenotrophomonas maltophilia
1.1.1.169 NADPH preferred Salmonella enterica subsp. enterica serovar Typhimurium