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Literature summary extracted from

  • Miyoshi, T.; Sato, H.; Harada, T.
    Purification and characterization of 2-alkyne-1-ol dehydrogenase induced by 2-butyne-1,4-diol in Fusarium merismoides B11 (1974), Biochim. Biophys. Acta, 358, 231-239.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.165 highly labile, even in the presence of DTT Fusarium merismoides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.165 CuCl2 complete inhibition at 2 mM Fusarium merismoides
1.1.1.165 HgCl2 complete inhibition at 2 mM Fusarium merismoides
1.1.1.165 iodoacetic acid complete inhibition at 2 mM Fusarium merismoides
1.1.1.165 KCN slight inhibition at 2 mM Fusarium merismoides
1.1.1.165 NaN3 slight inhibition at 2 mM Fusarium merismoides
1.1.1.165 Ni2+ 84% inhibition at 2 mM Fusarium merismoides
1.1.1.165 p-chloromercuribenzoate complete inhibition at 2 mM Fusarium merismoides
1.1.1.165 Zn2+ 55% inhibition at 2 mM Fusarium merismoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.165 9.1
-
 2-butyne-1,4-diol
-
 Fusarium merismoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.165 2-butyne-1,4-diol + NAD+ Fusarium merismoides
-
 4-hydroxybut-2-ynal + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.165 Fusarium merismoides
-
 strainB11
-
1.1.1.165 Fusarium merismoides B11
-
 strainB11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.165 to homogeneity, chromatography techniques Fusarium merismoides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.165 culture condition:2-butyne-1,4-diol-grown cell highest induction of enzyme formation by use of 2-butyne-1,4-diol-containing growth media Fusarium merismoides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.165 751.1
-
-
 Fusarium merismoides

Storage Stability

EC Number Storage Stability Organism
1.1.1.165 5°C, 2 days Fusarium merismoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.165 1,2,4-butanetriol + NAD+ low activity Fusarium merismoides ? + NADH
-
 ?
1.1.1.165 1,2,4-butanetriol + NAD+ low activity Fusarium merismoides B11 ? + NADH
-
 ?
1.1.1.165 1,2-butanediol + NAD+ low activity Fusarium merismoides 1-hydroxybutan-2-one + NADH
-
 ?
1.1.1.165 1,2-butanediol + NAD+ low activity Fusarium merismoides B11 1-hydroxybutan-2-one + NADH
-
 ?
1.1.1.165 1,2-propanediol + NAD+
-
 Fusarium merismoides 1-hydroxy-2-propanal + NADH
-
 ?
1.1.1.165 1,2-propanediol + NAD+
-
 Fusarium merismoides B11 1-hydroxy-2-propanal + NADH
-
 ?
1.1.1.165 1,4-butanediol + NAD+
-
 Fusarium merismoides 1-hydroxy-4-butanal + NADH
-
 ?
1.1.1.165 1,4-butanediol + NAD+
-
 Fusarium merismoides B11 1-hydroxy-4-butanal + NADH
-
 ?
1.1.1.165 1,6-hexanediol + NAD+ low activity Fusarium merismoides 1-hydroxy-6-hexanal + NADH
-
 ?
1.1.1.165 1-butene-3-ol + NAD+ low activity Fusarium merismoides 1-buten-3-one + NADH
-
 ?
1.1.1.165 1-butyne-3-ol + NAD+ very low activity Fusarium merismoides 1-butyne-3-one + NADH
-
 ?
1.1.1.165 2-butene-1-ol + NAD+ cis-trans mixture, very low activity Fusarium merismoides 2-butene-1-al + NADH
-
 ?
1.1.1.165 2-butyne-1,4-diol + NAD+
-
 Fusarium merismoides 4-hydroxybut-2-ynal + NADH
-
 ?
1.1.1.165 2-butyne-1-ol + NAD+ highest activity Fusarium merismoides 2-butyn-1-al + NADH
-
 ?
1.1.1.165 2-propene-1-ol + NAD+ low activity Fusarium merismoides 2-propene-1-al + NADH
-
 ?
1.1.1.165 2-propyne-1-ol + NAD+
-
 Fusarium merismoides 2-propyn-1-al + NADH
-
 ?
1.1.1.165 3-butyne-1-ol + NAD+ very low activity Fusarium merismoides 3-butyn-1-al + NADH
-
 ?
1.1.1.165 n-propanol + NAD+ low activity Fusarium merismoides n-propanal + NADH
-
 ?
1.1.1.165 n-propanol + NAD+ low activity Fusarium merismoides B11 n-propanal + NADH
-
 ?
1.1.1.165 trans-2-butene-1,4-diol + NAD+
-
 Fusarium merismoides ? + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.165 40
-
 stable below, rapid decrease of activity above Fusarium merismoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.165 7.5 8.5
-
 Fusarium merismoides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.165 5.8 8.5 stable between Fusarium merismoides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.165 NAD+
-
 Fusarium merismoides
1.1.1.165 NADP+ one third of the activity compared to NAD+ Fusarium merismoides