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Literature summary extracted from

  • Uehara, K.; Mannen, S.; Hosomi, S.; Miyashita, T.
    Studies on D-tetrose metabolism. Crystallization and properties of D-erythrulose reductase from chicken liver (1980), J. Biochem., 87, 47-55.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.162
-
Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.162 0.0079
-
NADPH
-
Gallus gallus
1.1.1.162 0.067
-
NADH
-
Gallus gallus
1.1.1.162 0.38
-
D-erythrulose
-
Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.162 22400
-
4 * 22400, SDS-PAGE Gallus gallus
1.1.1.162 96000
-
sedimentation equilibrium analysis Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.162 D-erythrulose + NAD(P)+ Gallus gallus one route of formation of tetritols in mammalia ?
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.162 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.162
-
Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.162 liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.162 205
-
-
Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.162 D-erythrulose + NAD(P)+ one route of formation of tetritols in mammalia Gallus gallus ?
-
r
1.1.1.162 D-erythrulose + NAD(P)H highly specific for D-erythrulose Gallus gallus D-threitol + NAD(P)+ reverse reaction rate is very low r
1.1.1.162 D-erythrulose + NAD(P)H the equilibrium of the reaction strongly favors the reduction of D-erythrulose, the reverse reaction is slightly detectable when carried out at pH 7.5-9.0 using NADP+, erythritol does not serve as substrate Gallus gallus D-threitol + NAD(P)+ reverse reaction rate is very low r
1.1.1.162 D-erythrulose + NADH + H+
-
Gallus gallus D-threitol + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.162 tetramer 4 * 22400, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.162 25
-
assay at Gallus gallus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.162 additional information
-
chicken: quite stable, even when a dilute solution at high ionic strength is incubated at low temperatures Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.162 5.8
-
D-erythrulose + NADPH Gallus gallus
1.1.1.162 6.3
-
D-erythrulose + NADH Gallus gallus
1.1.1.162 9
-
D-threitol + NADP+ Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.162 NADP+ enzyme contains 2-3 mol of bound NADP+ Gallus gallus