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Literature summary extracted from

  • Anwar, R.A.; Vlaovic, M.
    Purification of UDP-N-acetylenolpyruvoylglucosamine reductase from Escherichia coli by affinity chromatography, its subunit structure and the absence of flavin as the prosthetic group (1979), Can. J. Biochem., 57, 188-196.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.98 iodoacetate
-
 Escherichia coli
1.3.1.98 p-chloromercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.98 0.0046
-
 NADPH
-
 Escherichia coli
1.3.1.98 0.0065
-
 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.98 K+
-
 Escherichia coli
1.3.1.98 NH4+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.98 13500
-
 1 * 21500 + 1 * 13500, SDS-PAGE Escherichia coli
1.3.1.98 21500
-
 1 * 21500 + 1 * 13500, SDS-PAGE Escherichia coli
1.3.1.98 35000
-
 gel filtration Escherichia coli
1.3.1.98 35500
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Escherichia coli
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.98 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.98
-
 Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.98 3.32
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.3.1.98 -20°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Escherichia coli UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.98 heterodimer 1 * 21500 + 1 * 13500, SDS-PAGE Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.98 7.8 8.4
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.98 additional information absorption spectra not consistent with flavoprotein Escherichia coli
1.3.1.98 additional information 3'-NADPH, alpha-NADPH are substrates Escherichia coli
1.3.1.98 NADPH
-
 Escherichia coli