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Literature summary extracted from

  • Boynton, Z.L.; Bennett, G.N.; Rudolph, F.B.
    Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824 (1996), J. Bacteriol., 178, 3015-3024.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.1 expression in Escherichia coli Clostridium acetobutylicum
4.2.1.150 expressed in Escherichia coli XL1-Blue cells Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.150 28200
-
 x * 28200, calculated from amino acid sequence Clostridium acetobutylicum
4.2.1.150 29000
-
 x * 29000, SDS-PAGE Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Clostridium acetobutylicum
-
 ATCC 824
-
4.2.1.150 Clostridium acetobutylicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.150
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.1 1.2
-
 enzyme activity in crude extracts Clostridium acetobutylicum
1.3.8.1 3.3
-
 enzyme activity in crude extracts of cells harboring a plasmid with cloned gene Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.150 crotonyl-CoA + H2O
-
 Clostridium acetobutylicum 3-hydroxybutyryl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.150 ? x * 29000, SDS-PAGE Clostridium acetobutylicum
4.2.1.150 ? x * 28200, calculated from amino acid sequence Clostridium acetobutylicum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.150 crotonase
-
 Clostridium acetobutylicum
4.2.1.150 crt
-
 Clostridium acetobutylicum