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Literature summary extracted from

  • Katoh, S.
    Sepiapterin reductase from horse liver: purification and properties of the enzyme (1971), Arch. Biochem. Biophys., 146, 202-214.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.153 bovine serum albumin stabilizes Equus caballus
1.1.1.153 NADP+ stabilizes Equus caballus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.153 2,4-dinitrophenol 37% inhibition of the reductive reaction at 0.01 mM; slight Equus caballus
1.1.1.153 2,4-dinitrophenol
-
 Rattus norvegicus
1.1.1.153 6,7-Diethylpterin-H2
-
 Equus caballus
1.1.1.153 6,7-dimethyldihydropterin 0.05 mM: 70% inhibition Equus caballus
1.1.1.153 6,7-dimethyllumazine slight Equus caballus
1.1.1.153 6,7-dimethylpterin 0.05 mM: 46% inhibition; 46% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 6,7-dimethylpterin-H2 70% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 6-Carboxypterin 77% inhibition of the reductive reaction at 0.05 mM; IC50 0.014 mM, 0.05 mM: 77% inhibition Equus caballus
1.1.1.153 6-Carboxypterin
-
 Rattus norvegicus
1.1.1.153 6-Hydroxymethylpterin 0.05 mM: 23% inhibition; 23% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 6-methyldihydropterin 0.05 mM: 59% inhibition Equus caballus
1.1.1.153 6-methylpterin 10% inhibition of the reductive reaction at 0.05 mM; slight Equus caballus
1.1.1.153 6-Methylpterin-H2 59% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 7-Methylpterin 0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 acetic acid IC50 0.45 mM, pH 6.5 Equus caballus
1.1.1.153 benzoic acid 0.03 mM: 40% inhibition; 40% inhibition of the reductive reaction at 0.03 mM Equus caballus
1.1.1.153 biopterin 0.05 mM: 24% inhibition; 24% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 biopterin-H2 31% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 D,L-Lactic acid 0.03 mM, 20% inhibition Equus caballus
1.1.1.153 D-erythro-neopterin 14% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 D-erythro-neopterin-H2 7% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 dihydrobiopterin 0.05 mM: 31% inhibition Equus caballus
1.1.1.153 dihydrobiopterin
-
 Rattus norvegicus
1.1.1.153 dihydroxanthopterin slight Equus caballus
1.1.1.153 DL-lactic acid 20% inhibition of the reductive reaction at 0.03 mM Equus caballus
1.1.1.153 formic acid slight Equus caballus
1.1.1.153 iodoacetate 72% inhibition of the reductive reaction at 0.1 mM Equus caballus
1.1.1.153 Isosepiapterin 83% inhibition of the reductive reaction at 0.05 mM; IC50 0.0065 mM, 0.05 mM: 83% inhibition Equus caballus
1.1.1.153 Isoxanthopterin 0.05 mM: very slight Equus caballus
1.1.1.153 KCN 25% inhibition of the reductive reaction at 2.5 mM; slight Equus caballus
1.1.1.153 L-erythro-dihydroneopterin 0.05 mM: 29% inhibition, D-isomer: slight inhibition Equus caballus
1.1.1.153 L-Erythro-neopterin 0.05 mM: 21% inhibition, D-isomer: slight inhibition; 21% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 L-Erythro-neopterin-H2 29% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 Leucopterin 0.05 mM: 21% inhibition; 21% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 monoiodoacetate
-
 Equus caballus
1.1.1.153 additional information other unconjugated pteridines, e.g. xanthopterin, that cause slight inhibition of enzymatic reduction Equus caballus
1.1.1.153 n-butyric acid IC50 0.032 mM, pH 6.5 Equus caballus
1.1.1.153 N-ethylmaleimide
-
 Equus caballus
1.1.1.153 n-valeric acid IC50 0.25 mM, pH 6.5 Equus caballus
1.1.1.153 NADPH above 0.1 mM Equus caballus
1.1.1.153 NaN3 15% inhibition of the reductive reaction at 5 mM; slight Equus caballus
1.1.1.153 NEM 35% inhibition of the reductive reaction at 0.1 mM, 88% at 1 mM Equus caballus
1.1.1.153 p-chloromercuribenzoate
-
 Equus caballus
1.1.1.153 PCMB 93% inhibition of the reductive reaction at 0.01 mM Equus caballus
1.1.1.153 Propionamide 0.03 mM, 14% inhibition; 14% inhibition of the reductive reaction at 0.03 mM Equus caballus
1.1.1.153 propionic acid 43% inhibition of the reductive reaction at 0.03 mM; IC50 0.0045 mM, pH 6.5 Equus caballus
1.1.1.153 pterin 0.05 mM: 18% inhibition; 18% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 Pyruvic acid 9% inhibition of the reductive reaction at 0.03 mM; slight Equus caballus
1.1.1.153 sepiapterin above 0.05 mM Equus caballus
1.1.1.153 sepiapterin
-
 Rattus norvegicus
1.1.1.153 Sodium acetate
-
 Equus caballus
1.1.1.153 Xanthopterin slight Equus caballus
1.1.1.153 Xanthopterin B2 slight Equus caballus
1.1.1.153 Xanthopterin-B2 12% inhibition of the reductive reaction at 0.05 mM Equus caballus
1.1.1.153 xanthopterin-H2 9% inhibition of the reductive reaction at 0.05 mM Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.153 additional information
-
 additional information kinetics Equus caballus
1.1.1.153 0.0014
-
 NADPH pH 10.4, 25°C Equus caballus
1.1.1.153 0.0021
-
 sepiapterin pH 10.4, 25°C Equus caballus
1.1.1.153 0.0057
-
 NADP+ pH 10.4, 25°C Equus caballus
1.1.1.153 0.0077
-
 7,8-dihydrobiopterin pH 10.4, 25°C Equus caballus
1.1.1.153 0.014
-
 NADPH
-
 Equus caballus
1.1.1.153 0.021
-
 sepiapterin
-
 Equus caballus
1.1.1.153 0.057
-
 dihydrobiopterin at pH 10.4 Equus caballus
1.1.1.153 0.077
-
 NADP+ at pH 10.4 Equus caballus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.153 cytoplasm 92% of total activity Equus caballus 5737
-
1.1.1.153 additional information intracellular localization, overview Equus caballus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus equilibrium lies much in favor of dihydrobiopterin formation 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Equus caballus equilibrium lies much in favor of dihydrobiopterin formation 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Equus caballus reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 sepiapterin + NADPH + H+ Equus caballus
-
 7,8-dihydrobiopterin + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Equus caballus
-
-
-
1.1.1.153 Rattus norvegicus
-
-
-
1.1.1.153 Rattus norvegicus
-
 female and male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153 liver Equus caballus
1.1.1.153 native enzyme 5000fold from liver by ammonium sulfate and protamine sulfate fractionation, and two times treatment by hydroxylapatite and anion exchange chromatography Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.153 blood
-
 Rattus norvegicus
-
1.1.1.153 blood
-
 Equus caballus
-
1.1.1.153 brain
-
 Rattus norvegicus
-
1.1.1.153 erythrocyte
-
 Rattus norvegicus
-
1.1.1.153 erythrocyte
-
 Equus caballus
-
1.1.1.153 heart
-
 Rattus norvegicus
-
1.1.1.153 kidney
-
 Rattus norvegicus
-
1.1.1.153 liver
-
 Rattus norvegicus
-
1.1.1.153 liver
-
 Equus caballus
-
1.1.1.153 additional information tissue distribution, no activity in stomach, small intestine, and muscle, overview Rattus norvegicus
-
1.1.1.153 spleen
-
 Rattus norvegicus
-
1.1.1.153 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.153 additional information
-
 purified enzyme Equus caballus

Storage Stability

EC Number Storage Stability Organism
1.1.1.153 -15°C, in 150 mM Tris-HCl buffer, pH 6.0, second hydroxylapatite fraction, for 1 week, relatively little loss of activity Equus caballus
1.1.1.153 -15°C, purified enzyme, 0.01 M potassium phosphate buffer, pH 6.0, stable for 1 week with little loss of activity Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH in the reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding L-isomers are inactive Equus caballus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH equilibrium lies much in favor of dihydrobiopterin formation Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH equilibrium lies much in favor of dihydrobiopterin formation Equus caballus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH reverse reaction, L-erythro and L-threo-dihydroneopterin are also oxidized, the corresponding D-isomers are inactive Equus caballus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 r
1.1.1.153 isosepiapterin + NADPH
-
 Equus caballus 6-(1-hydroxypropyl)-dihydropterin + NADP+
-
 ?
1.1.1.153 isosepiapterin + NADPH
-
 Equus caballus ? + NADP+
-
 r
1.1.1.153 L-erythro-dihydroneopterin + NADP+ D-isomer completely inactive Equus caballus ?
-
 r
1.1.1.153 L-erythro-dihydroneopterin + NADP+ very slow activity, no activity with the D-isomer Equus caballus ?
-
 r
1.1.1.153 L-threo-dihydroneopterin + NADP+ D-isomer completely inactive Equus caballus ?
-
 r
1.1.1.153 L-threo-dihydroneopterin + NADP+ very slow activity, no activity with the D-isomer Equus caballus ?
-
 r
1.1.1.153 additional information not: 6-acetyl-7-methyldihydropterin Rattus norvegicus ?
-
 ?
1.1.1.153 additional information not: 6-acetyl-7-methyldihydropterin Equus caballus ?
-
 ?
1.1.1.153 additional information no activity with 6-acetyl-7-methyl-7,8-dihydropterin Equus caballus ?
-
 ?
1.1.1.153 sepiapterin + NADPH + H+
-
 Rattus norvegicus 7,8-dihydrobiopterin + NADP+
-
 r
1.1.1.153 sepiapterin + NADPH + H+
-
 Equus caballus 7,8-dihydrobiopterin + NADP+
-
 r
1.1.1.153 xanthopterin B2 + NADPH
-
 Equus caballus 6-(1',2'-dihydroxypropyl)-dihydrolumazine + NADP+
-
 ?
1.1.1.153 xanthopterin-B2 + NADPH
-
 Equus caballus ? + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.153 SR
-
 Rattus norvegicus
1.1.1.153 SR
-
 Equus caballus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.153 25
-
 assay at Rattus norvegicus
1.1.1.153 25
-
 assay at Equus caballus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.153 5.5
-
 in potassium phosphate buffer Rattus norvegicus
1.1.1.153 5.5
-
 in potassium phosphate buffer Equus caballus
1.1.1.153 5.5
-
 for reduction of sepiapterin Equus caballus
1.1.1.153 6.4
-
 substrate reduction reaction, assay at Rattus norvegicus
1.1.1.153 6.4
-
 substrate reduction reaction, assay at Equus caballus
1.1.1.153 10.4
-
 the reverse reaction, oxidation of 7,8-dihydrobiopterin, activity rises with rise of pH Equus caballus
1.1.1.153 10.4
-
 substrate oxidation reaction, assay at Rattus norvegicus
1.1.1.153 10.4
-
 substrate oxidation reaction, assay at Equus caballus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.153 5.5
-
 unstable Rattus norvegicus
1.1.1.153 5.5
-
 unstable Equus caballus
1.1.1.153 5.5
-
 pH 6.4 for enzyme assay Equus caballus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 NAD+ reverse reaction, one-fifth of that with NADP+ Equus caballus
1.1.1.153 NADH
-
 Rattus norvegicus
1.1.1.153 NADH activity one-fourth of that with NADPH Equus caballus
1.1.1.153 NADP+
-
 Rattus norvegicus
1.1.1.153 NADP+
-
 Equus caballus
1.1.1.153 NADPH
-
 Rattus norvegicus
1.1.1.153 NADPH
-
 Equus caballus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.153 0.0045
-
 IC50 0.0045 mM, pH 6.5 Equus caballus propionic acid
1.1.1.153 0.0065
-
 IC50 0.0065 mM, 0.05 mM: 83% inhibition Equus caballus Isosepiapterin
1.1.1.153 0.014
-
 IC50 0.014 mM, 0.05 mM: 77% inhibition Equus caballus 6-Carboxypterin
1.1.1.153 0.032
-
 IC50 0.032 mM, pH 6.5 Equus caballus n-butyric acid
1.1.1.153 0.25
-
 IC50 0.25 mM, pH 6.5 Equus caballus n-valeric acid
1.1.1.153 0.45
-
 IC50 0.45 mM, pH 6.5 Equus caballus acetic acid