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Literature summary extracted from

  • Monder, C.; White, A.
    The 21-hydroxysteroid dehydrogenases of liver. A nicotinamide adenine dinucleotide phosphate dehydrogenase and two nicotinamide adenine dinucleotide dehydrogenases (1965), J. Biol. Chem., 240, 71-77.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.151 not sensitive against repeated freezing and thawing Ovis aries aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.150 Ag+ complete inhibition of NAD dehydrogenase II at 0.018 mM Ovis aries aries
1.1.1.150 Hg2+ 40% inhibition of NAD dehydrogenase II at 0.004 mM Ovis aries aries
1.1.1.150 NAD+ NAD dehydrogenase II, competitive inhibition Ovis aries aries
1.1.1.150 NADP+ NAD dehydrogenase II, competitive inhibition Ovis aries aries
1.1.1.150 p-mercuribenzoate 79% inhibition of NAD dehydrogenase II at 0.014 mM Ovis aries aries
1.1.1.151 Ag+ complete inhibition at 0.004 mM Ovis aries aries
1.1.1.151 Hg2+ 53% inhibition at 0.002 mM Ovis aries aries
1.1.1.151 NADH competitive inhibition Ovis aries aries
1.1.1.151 NADPH competitive inhibition Ovis aries aries
1.1.1.151 p-mercuribenzoate 55% inhibition at 0.014 mM Ovis aries aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.150 0.01
-
 NADH NAD dehydrogenase I Ovis aries aries
1.1.1.150 0.048
-
 NADH NAD dehydrogenase II Ovis aries aries
1.1.1.150 0.105
-
 11beta-hydroxy-pregn-4-ene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
1.1.1.150 0.111
-
 17-hydroxy-pregn-4-ene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
1.1.1.150 0.172
-
 11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
1.1.1.150 0.174
-
 pregn-4-ene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
1.1.1.150 0.182
-
 11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
1.1.1.150 0.221
-
 17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
 Ovis aries aries
1.1.1.151 0.021
-
 NADPH
-
 Ovis aries aries
1.1.1.151 0.118
-
 pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
1.1.1.151 0.137
-
 11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione
-
 Ovis aries aries
1.1.1.151 0.137
-
 17-hydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
1.1.1.151 0.177
-
 11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
1.1.1.151 0.233
-
 11beta-hydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
1.1.1.151 0.461
-
 17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
 Ovis aries aries

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.150 Ovis aries aries
-
 sheep
-
1.1.1.151 Ovis aries aries
-
 sheep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.150 to homogeneity, gel filtration, starch block electrophoresis Ovis aries aries
1.1.1.151 to homogeneity, Sephadex G-100, starch block elelctrophoresis Ovis aries aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.150 liver 2 isoenzymes: NAD dehydrogenase I and II Ovis aries aries
-
1.1.1.151 liver
-
 Ovis aries aries
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.151 -10°C, 0.01 M sodium phosphate , pH 6-7.4, 1 year Ovis aries aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.150 11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADH i.e. 21-dehydro-delta1-cortisol, only NAD dehydrogenase II Ovis aries aries 11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NAD+ i.e. 21-hydroxy-delta1-cortisol ?
1.1.1.150 11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADH
-
 Ovis aries aries 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. cortisol ?
1.1.1.150 11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADH i.e. 21-dehydrocorticosterone, only NAD dehydrogenase II Ovis aries aries 11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. 21-hydroxycorticosterone ?
1.1.1.150 17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADH i.e. 21-dehydrocortisone Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NAD+ i.e. cortisone ?
1.1.1.150 17-hydroxy-pregn-4-ene-3,20,21-trione + NADH i.e. 21-dehydro-11-deoxycortisol, only NAD dehydrogenase II Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. 21-hydroxy-11-deoxycortisol ?
1.1.1.150 pregn-4-ene-3,20,21-trione + NADH i.e. 21-dehydro-11-deoxycorticosterone, only NAD dehydrogenase II Ovis aries aries 21-hydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. 21-hydroxy-11-deoxycorticosterone ?
1.1.1.151 11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADPH i.e. 21-dehydro-delta1-cortisol Ovis aries aries 11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NADP+ 21-hydroxy-delta1-cortisol ?
1.1.1.151 11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydrocortisol Ovis aries aries 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. cortisol ?
1.1.1.151 11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydrocorticosterone Ovis aries aries 11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+ 21-hydroxycorticosterone ?
1.1.1.151 17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH + H+
-
 Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+
-
 r
1.1.1.151 17-hydroxy-pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydro-11-deoxycortisol Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,20-dione + NADP+ i.e. 21-hydroxy-11-deoxycortisol ?
1.1.1.151 17alpha-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADPH i.e. 21-dehydrocortisone, marked dependence on the nature of steroids Ovis aries aries 17alpha,21-dihydroxy-pregn-4-ene-3,11,20-trione + NADP+ i.e. cortisone ?
1.1.1.151 pregn-4-ene-3,20,21-trione + NADPH i.e. 21-dehydro-11-deoxy-corticosterone Ovis aries aries 21-hydroxy-pregn-4-ene-3,20-dione + NADP+ 21-hydroxy-11-deoxy-corticosterone ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.150 56
-
 30% loss of activity in 5 min, NAD dehydrogenase I more heat resistant than NAD dehydrogenase II Ovis aries aries
1.1.1.151 56
-
 80% loss of activity in 5 min Ovis aries aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.150 6.2
-
 NAD dehydrogenase II Ovis aries aries
1.1.1.150 6.9
-
 NAD dehydrogenase I Ovis aries aries
1.1.1.151 5.9 6.1
-
 Ovis aries aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.150 NADH
-
 Ovis aries aries
1.1.1.151 NADPH
-
 Ovis aries aries