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Literature summary extracted from

  • Vongsuvanlert, V.; Tani, Y.
    Characterization of D-sorbitol dehydrogenase involved in D-sorbitol production of a methanol yeast, Candida boidinii (Kloeckera sp.) No. 2201 (1988), Agric. Biol. Chem., 52, 419-426.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.14 Semicarbazide 1 mM [Candida] boidinii
1.1.1.14 Sodium azide 10 mM stimulates 2fold [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 Ba(CH3COO)2 1 mM reduces activity to about 20% [Candida] boidinii
1.1.1.14 CoCl2
-
 [Candida] boidinii
1.1.1.14 dithiothreitol
-
 [Candida] boidinii
1.1.1.14 EDTA
-
 [Candida] boidinii
1.1.1.14 Na2SO4
-
 [Candida] boidinii
1.1.1.14 NiCl2 1 mM reduces activity to about 20% [Candida] boidinii
1.1.1.14 o-phenanthroline
-
 [Candida] boidinii
1.1.1.14 Pb(CH3COO)2
-
 [Candida] boidinii
1.1.1.14 Semicarbazide 10 mM [Candida] boidinii
1.1.1.14 ZnSO4
-
 [Candida] boidinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.14 Fe3+ 10 mM FeCl3 stimulates activity 2 fold [Candida] boidinii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 [Candida] boidinii
-
 (Kloeckera sp.) No. 2201
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 30.94
-
 partially purified [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-arabinose + NADH low activity [Candida] boidinii ?
-
 ?
1.1.1.14 D-arabitol + NAD+ D-arabinitol is identical, low activity [Candida] boidinii ?
-
 ?
1.1.1.14 D-fructose + NADH + H+
-
 [Candida] boidinii D-sorbitol + NAD+
-
 ?
1.1.1.14 D-glucose + NADH low activity [Candida] boidinii ?
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 [Candida] boidinii ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 [Candida] boidinii ?
-
 ?
1.1.1.14 D-ribose + NADH low activity [Candida] boidinii ?
-
 ?
1.1.1.14 D-ribulose + NADH
-
 [Candida] boidinii ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 [Candida] boidinii D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 [Candida] boidinii ?
-
 ?
1.1.1.14 D-xylose + NADH low activity [Candida] boidinii ?
-
 ?
1.1.1.14 D-xylulose + NADH
-
 [Candida] boidinii ?
-
 ?
1.1.1.14 L-iditol + NAD+ low activity [Candida] boidinii ?
-
 ?
1.1.1.14 L-rhamnose + NADH low activity [Candida] boidinii ?
-
 ?
1.1.1.14 L-sorbose + NADH low activity [Candida] boidinii ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.14 37
-
-
 [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 5.6
-
 D-fructose reduction [Candida] boidinii
1.1.1.14 9.5
-
 sorbitol oxidation [Candida] boidinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.14 7 8 12 h at 4°C [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+ preferred [Candida] boidinii
1.1.1.14 NADH preferred [Candida] boidinii
1.1.1.14 NADP+
-
 [Candida] boidinii
1.1.1.14 NADPH
-
 [Candida] boidinii