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Literature summary extracted from

  • Marini, I.; Bucchioni, L.; Borella, P.; Del Corso, A.; Mura, U.
    Sorbitol dehydrogenase from bovine lens: purification and properties (1997), Arch. Biochem. Biophys., 340, 383-391.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.14 freezing, loss of activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 1,10-phenanthroline
-
 Bos taurus
1.1.1.14 EDTA
-
 Bos taurus
1.1.1.14 quercetin
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 0.052
-
 NADH
-
 Bos taurus
1.1.1.14 0.089
-
 NAD+
-
 Bos taurus
1.1.1.14 3.2
-
 D-xylitol
-
 Bos taurus
1.1.1.14 18
-
 D-sorbitol
-
 Bos taurus
1.1.1.14 20
-
 L-Iditol
-
 Bos taurus
1.1.1.14 67
-
 D-ribitol
-
 Bos taurus
1.1.1.14 180
-
 D-fructose
-
 Bos taurus
1.1.1.14 260
-
 D-ribulose
-
 Bos taurus
1.1.1.14 268
-
 L-Threitol
-
 Bos taurus
1.1.1.14 381
-
 D-mannitol
-
 Bos taurus
1.1.1.14 670
-
 L-sorbose
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.14 Zn 0.83 mol per subunit Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 39000
-
 4 * 39000, SDS-PAGE Bos taurus
1.1.1.14 156000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.14
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 lens
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 52
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.1.1.14 4°C, 2 mM dithiothreitol, 0.1 mM NADH, 15 days Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
 Bos taurus D-sorbitol + NAD+
-
 ?
1.1.1.14 D-mannitol + NAD+ low activity Bos taurus ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Bos taurus ?
-
 ?
1.1.1.14 D-ribulose + NADH
-
 Bos taurus ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Bos taurus D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Bos taurus ?
-
 ?
1.1.1.14 L-iditol + NAD+
-
 Bos taurus ?
-
 ?
1.1.1.14 L-sorbose + NADH
-
 Bos taurus ?
-
 ?
1.1.1.14 L-threitol + NAD+ low activity Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.14 tetramer 4 * 39000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 7.4
-
 D-fructose reduction Bos taurus
1.1.1.14 9
-
 sorbitol oxidation Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+ specific for Bos taurus
1.1.1.14 NADH specific for Bos taurus