EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.14 | ZnSO4 | reverses cysteine inhibition | Malus domestica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.14 | AgNO3 | - |
Malus domestica | |
1.1.1.14 | cysteine | strong effect on sorbitol oxidation, slight effect on fructose reduction, ZnSO4 reverses inhibition | Malus domestica | |
1.1.1.14 | HgCl2 | - |
Malus domestica | |
1.1.1.14 | iodoacetate | strong effect on sorbitol oxidation, weaker effect on fructose reduction | Malus domestica | |
1.1.1.14 | p-chloromercuribenzoate | - |
Malus domestica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.14 | 37 | - |
D-xylitol | - |
Malus domestica | |
1.1.1.14 | 86 | - |
D-sorbitol | - |
Malus domestica | |
1.1.1.14 | 1500 | - |
D-fructose | - |
Malus domestica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.14 | Malus domestica | - |
apple | - |
1.1.1.14 | Malus domestica | - |
cv Granny Smith | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.14 | mesocarp | tissue culture | Malus domestica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.14 | D-fructose + NADH + H+ | - |
Malus domestica | D-sorbitol + NAD+ | - |
? | |
1.1.1.14 | D-mannitol + NAD+ | - |
Malus domestica | ? | - |
? | |
1.1.1.14 | D-ribitol + NAD+ | - |
Malus domestica | ? | - |
? | |
1.1.1.14 | D-sorbitol + NAD+ | - |
Malus domestica | D-fructose + NADH + H+ | - |
? | |
1.1.1.14 | D-xylitol + NAD+ | - |
Malus domestica | ? | - |
? | |
1.1.1.14 | L-arabitol + NAD+ | - |
Malus domestica | ? | - |
? | |
1.1.1.14 | L-sorbose + NADH | - |
Malus domestica | ? | - |
? | |
1.1.1.14 | L-threitol + NAD+ | - |
Malus domestica | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 60 | - |
- |
Malus domestica |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 35 | 65 | 35°C: about 50% of activity maximum, 65°C: about 95% of activity maximum | Malus domestica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 5.7 | 7 | D-fructose reduction | Malus domestica |
1.1.1.14 | 9 | 10 | sorbitol oxidation | Malus domestica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 4.7 | 8 | at pH 4.7 and 8 about 50% of activity maximum, D-fructose reduction | Malus domestica |
1.1.1.14 | 8 | 11 | at pH 8 and 11 about 50% of activity maximum, sorbitol oxidation | Malus domestica |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.14 | 4.5 | - |
at lower pH denaturation occurs | Malus domestica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.14 | NAD+ | - |
Malus domestica | |
1.1.1.14 | NADH | - |
Malus domestica | |
1.1.1.14 | NADP+ | cannot replace NAD+ | Malus domestica | |
1.1.1.14 | NADPH | less effective than NADH | Malus domestica |