Literature summary extracted from

Stoop, J.M.H.; Mooibroek, H.
Cloning and characterization of NADP-dependent dehydrogenase cDNA from the button mushroom, Agaricus bisporus, and its expression in response to NaCl stress (1998), Appl. Environ. Microbiol., 64, 4689-4696.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.138	expression in Pichia pastoris, gene cloning in Escherichia coli	Agaricus bisporus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.138	29000	-	x * 29000, SDS-PAGE	Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.138	D-mannitol + NADP+	Agaricus bisporus	-	D-fructose + NADPH + H+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.138	Agaricus bisporus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.138	to homogeneity, chromatography techniques	Agaricus bisporus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.138	fruit body	stage 2, highest activity in salt stressed fruit bodies	Agaricus bisporus	-
1.1.1.138	mycelium	-	Agaricus bisporus	-

Storage Stability

EC Number	Storage Stability	Organism
1.1.1.138	-80°C, 50 mM MOPS, pH 7.5, 1 mM DTT, 50 mM NaCl, several weeks	Agaricus bisporus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.138	D-mannitol + NADP+	-	Agaricus bisporus	D-fructose + NADPH + H+	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.138	?	x * 29000, SDS-PAGE	Agaricus bisporus

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.138	NADP-mannitol dehydrogenase	-	Agaricus bisporus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.138	NADP+	specific for NADP+	Agaricus bisporus

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Release 2023.1 (January 2023)