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Literature summary extracted from

  • Giraud, M.F.; McMiken, H.J.; Leonard, G.A.; Messner, P.; Whitfield, C.; Naismith, J.H.
    Overexpression, purification, crystallization and preliminary structural study of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD), the fourth enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar typhimurium (1999), Acta Crystallogr. Sect. D, 55, 2043-2046.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.133 native overexpressed in Escherichia coli BL21(DE3) and selenomethionine-enriched in B834(lambdaDE3) Salmonella enterica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.133 sitting drop vapour-diffusion method, 6 d Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.133 32500
-
HPLC Salmonella enterica
1.1.1.133 32540
-
mass spectrometry, native protein Salmonella enterica
1.1.1.133 32560
-
deduced from sequence Salmonella enterica
1.1.1.133 32730
-
mass spectrometry, selenomethionine-enriched protein Salmonella enterica
1.1.1.133 49500
-
light-scattering Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Escherichia coli synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Xanthomonas campestris synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Salmonella enterica synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Shigella flexneri synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Streptomyces mutans synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.133 Escherichia coli
-
K12
-
1.1.1.133 Salmonella enterica
-
-
-
1.1.1.133 Shigella flexneri
-
-
-
1.1.1.133 Streptomyces mutans
-
-
-
1.1.1.133 Xanthomonas campestris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.133 anion-exchange, hydrophobic chromatography Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Escherichia coli dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Xanthomonas campestris dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Salmonella enterica dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Shigella flexneri dTDP-L-rhamnose + NADP+
-
?
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Streptomyces mutans dTDP-L-rhamnose + NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.133 dTDP-6-deoxy-L-lyxo-4-hexulose reductase
-
Salmonella enterica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.133 NADPH
-
Escherichia coli
1.1.1.133 NADPH
-
Xanthomonas campestris
1.1.1.133 NADPH
-
Salmonella enterica
1.1.1.133 NADPH
-
Shigella flexneri
1.1.1.133 NADPH
-
Streptomyces mutans