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Literature summary extracted from

  • Horan, N.J.; Jarman, T.R.; Dawes, E.A.
    Studies on some enzymes of alginic acid biosynthesis in Azotobacter vinelandii grown in continuous culture (1983), J. Gen. Microbiol., 129, 2985-2990.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.132 Azotobacter vinelandii
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SM52B, mutant of A. vinelandii NCIB 9068, alginate-prducing ability, no activity with sorbitol as sole carbon source during batch growth, enzyme not produced, loss of ability to synthesise alginate during prolonged continuous culture after 300 h for mutant and more than 1000 h for parent strain, ability transferable by conjugation
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.132 0.002
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inorganic phosphate limitation, 85 h after changing carbon source from sorbitol to sucrose Azotobacter vinelandii
1.1.1.132 0.0025
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oxygen limitation Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.132 GDP-mannose + NAD+ + H2O
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Azotobacter vinelandii GDP-mannuronate + NADH
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?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.132 NAD+
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Azotobacter vinelandii