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Literature summary extracted from

  • Condemine, G.; Hugouvieux-Cotte-Pattat, N.; Robert-Baudouy, J.
    An enzyme in the pectinolytic pathway of Erwinia chrysanthemi: 2-Keto-3-deoxygluconate oxidoreductase (1984), J. Gen. Microbiol., 130, 2839-2844.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.127 Ag+ 72% inhibition at 0.01 mM Dickeya chrysanthemi
1.1.1.127 ATP 30% inhibition for both reduction and oxidation reaction at 1 mM Dickeya chrysanthemi
1.1.1.127 p-chloromercuribenzoate 50% inhibition at 0.02 mM Dickeya chrysanthemi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.127 0.03
-
 NADH
-
 Dickeya chrysanthemi
1.1.1.127 0.34
-
 (4S)-4,6-dihydroxy-2,5-dioxohexanoate
-
 Dickeya chrysanthemi
1.1.1.127 0.4
-
 NAD+
-
 Dickeya chrysanthemi
1.1.1.127 7.7
-
 2-dehydro-3-deoxy-D-gluconate
-
 Dickeya chrysanthemi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.127 Ca2+ 20% activation at 1 mM Dickeya chrysanthemi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.127 2-dehydro-3-deoxy-D-gluconate + NAD+ Dickeya chrysanthemi involved in pectinolysis by some phytopathogenic bacteria (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.127 Dickeya chrysanthemi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.127 to homogeneity, 2step chromatography Dickeya chrysanthemi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.127 cell culture
-
 Dickeya chrysanthemi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.127 39
-
-
 Dickeya chrysanthemi

Storage Stability

EC Number Storage Stability Organism
1.1.1.127 -20°C, 2 mM DTT, 6 months Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.127 2-dehydro-3-deoxy-D-gluconate + NAD+ involved in pectinolysis by some phytopathogenic bacteria Dickeya chrysanthemi (4S)-4,6-dihydroxy-2,5-dioxohexanoate + NADH
-
 r
1.1.1.127 pyruvate + NAD+ 10% of the activity compared to 2-dehydro-3-deoxy-D-gluconate Dickeya chrysanthemi ? + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.127 45
-
-
 Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.127 20
-
 stable for 15 h Dickeya chrysanthemi
1.1.1.127 46
-
 half-life: 10 min, NADH increases thermal stability Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.127 7 7.5 reduction reaction Dickeya chrysanthemi
1.1.1.127 10
-
 oxidation reaction Dickeya chrysanthemi

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.127 NAD+
-
 Dickeya chrysanthemi
1.1.1.127 NADH
-
 Dickeya chrysanthemi
1.1.1.127 NADP+ 53% lower activity than with NAD+ Dickeya chrysanthemi
1.1.1.127 NADPH 62% lower activity than with NADH Dickeya chrysanthemi