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Literature summary extracted from

  • Englard, S.; Avigad, G.
    5-keto-D-Fructose reductase from Gluconobacter cerinus (1978), Methods Enzymol., 41B, 127-131.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.124 D-fructose inhibition of 5-keto-D-frucotse reduction, Ki: 82 mM Gluconobacter cerinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.124 0.002
-
NADPH
-
Gluconobacter cerinus
1.1.1.124 1.3
-
NADP+
-
Gluconobacter cerinus
1.1.1.124 4.2
-
5-Keto-D-fructose 1-phosphate
-
Gluconobacter cerinus
1.1.1.124 4.5
-
5-keto-D-fructose
-
Gluconobacter cerinus
1.1.1.124 70
-
D-fructose
-
Gluconobacter cerinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.124 D-fructose + NADP+ Gluconobacter cerinus predominant formation of D-fructose 5-dehydro-D-fructose + NADPH
-
r
1.1.1.124 D-fructose + NADP+ Gluconobacter cerinus highly specific for D-fructose 5-dehydro-D-fructose + NADPH
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.124 Gluconobacter cerinus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.124 high purity Gluconobacter cerinus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.124 cell culture
-
Gluconobacter cerinus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.124 627
-
-
Gluconobacter cerinus

Storage Stability

EC Number Storage Stability Organism
1.1.1.124 4°C, stable for 3-4 weeks Gluconobacter cerinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.124 5-keto-D-fructose 1-phosphate + NADPH + H+ 16% of the rate compared to 5-keto-D-fructose Gluconobacter cerinus D-fructose 1-phosphate + NADP+
-
?
1.1.1.124 D-fructose + NADP+ predominant formation of D-fructose Gluconobacter cerinus 5-dehydro-D-fructose + NADPH
-
r
1.1.1.124 D-fructose + NADP+ highly specific for D-fructose Gluconobacter cerinus 5-dehydro-D-fructose + NADPH
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.124 6.5
-
NADPH oxidation in phosphate buffer Gluconobacter cerinus
1.1.1.124 7.4
-
NADPH oxidation in Tris-HCl buffer Gluconobacter cerinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.124 NADPH cannot be replaced by NADH Gluconobacter cerinus