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Literature summary extracted from

  • Cline, A.L.; Hu, A.S.L.
    Some physical properties of three sugar dehydrogenases from a pseudomonad (1965), J. Biol. Chem., 240, 4498-4502.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.117 2.2
-
 NADP+
-
 Pseudomonas sp.
1.1.1.121 0.25
-
 NAD+
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.117 cytoplasm
-
 Pseudomonas sp. 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.117 20000
-
 x * 20000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible Pseudomonas sp.
1.1.1.117 50000
-
 gel filtration Pseudomonas sp.
1.1.1.117 104000
-
 sedimentation analysis in EDTA/phosphate buffer Pseudomonas sp.
1.1.1.121 38000
-
 x * 38000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible Pseudomonas sp.
1.1.1.121 40000
-
 gel filtration Pseudomonas sp.
1.1.1.121 140000
-
 sedimentation analysis in EDTA/phosphate buffer Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+ Pseudomonas sp.
-
 D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 D-arabinose + NAD(P)+ Pseudomonas sp. G6
-
 D-arabino-1,4-lactone + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.117 Pseudomonas sp.
-
 G6
-
1.1.1.117 Pseudomonas sp. G6
-
 G6
-
1.1.1.121 Pseudomonas sp.
-
 G6
-
1.1.1.121 Pseudomonas sp. G6
-
 G6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.117
-
 Pseudomonas sp.
1.1.1.121 G6 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.117 D-arabinose + NAD(P)+
-
 Pseudomonas sp. D-arabino-1,4-lactone + NAD(P)H
-
 ?
1.1.1.117 D-arabinose + NAD(P)+
-
 Pseudomonas sp. G6 D-arabino-1,4-lactone + NAD(P)H
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.117 ? x * 20000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible Pseudomonas sp.
1.1.1.121 ? x * 38000, sedimentation analysis in 4.2 M guanidinium chloride, presence of subunits of unequal size might be possible Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.117 58
-
 half-life of 20 s is increased to 80 s in presence of cofactor, in both cases biphasic inactivation curve Pseudomonas sp.
1.1.1.121 46
-
 half-life: 1 min, biphasic inactivation curve Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.117 NAD+
-
 Pseudomonas sp.
1.1.1.117 NADP+
-
 Pseudomonas sp.
1.1.1.121 NAD+
-
 Pseudomonas sp.