Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Goulas, P.
    Purification and properties of carnitine dehydrogenase from Pseudomonas putida (1988), Biochim. Biophys. Acta, 957, 335-339.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.108 0.013
-
 NADH potassium phosphate buffer, pH 7 Pseudomonas putida
1.1.1.108 0.067
-
 NADH Tris-HCl buffer, pH 9 Pseudomonas putida
1.1.1.108 0.2
-
 NAD+
-
 Pseudomonas putida
1.1.1.108 1.1
-
 3-dehydrocarnitine potassium phosphate buffer, pH 7 Pseudomonas putida
1.1.1.108 1.54
-
 3-dehydrocarnitine Tris-HCl buffer, pH 9 Pseudomonas putida
1.1.1.108 6.25
-
 L-carnitine
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.108 32000
-
 2 * 32000, SDS-PAGE Pseudomonas putida
1.1.1.108 60000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.108 (R)-3-hydroxy-4-trimethylaminobutyrate + NAD+ Pseudomonas putida
-
 3-dehydrocarnitine + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.108 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.108 ammonium sulfate, Sephadex G-200, 5'-AMP-Sepharose 4B, gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.108 55.3
-
-
 Pseudomonas putida
1.1.1.108 240
-
 reduction of 3-dehydrocarnitine at pH 7-7.5 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.108 (R)-3-hydroxy-4-trimethylaminobutyrate + NAD+
-
 Pseudomonas putida 3-dehydrocarnitine + NADH + H+
-
 r
1.1.1.108 carnitine + NAD+
-
 Pseudomonas putida 3-dehydrocarnitine + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.108 dimer 2 * 32000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.108 30
-
 activity declines rapidly above 35°C Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.108 10 30
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.108 7 7.5 reduction of 3-dehydrocarnitine Pseudomonas putida
1.1.1.108 9
-
 oxidation of L-carnitine Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.108 NAD+
-
 Pseudomonas putida