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Literature summary extracted from

  • Green, M.L.; Elliott, W.H.
    The enzymatic formation of aminoacetone from threonine and its further metabolism (1964), biochem. J., 92, 537-548.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.047
-
 NAD+
-
 Staphylococcus aureus
1.1.1.103 8.7
-
 L-threonine
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.103 L-threonine + NAD+ Gallus gallus
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+ Staphylococcus aureus
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+ Rattus norvegicus
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+ Sus scrofa
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+ Oryctolagus cuniculus
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Gallus gallus
-
-
-
1.1.1.103 Oryctolagus cuniculus
-
-
-
1.1.1.103 Rattus norvegicus
-
-
-
1.1.1.103 Staphylococcus aureus
-
-
-
1.1.1.103 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 MnCl2, ammonium sulfate, calcium phosphate gel, protamine sulfate, DEAE-cellulose, 7fold purification Staphylococcus aureus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.103 brain
-
 Rattus norvegicus
-
1.1.1.103 heart
-
 Rattus norvegicus
-
1.1.1.103 kidney
-
 Rattus norvegicus
-
1.1.1.103 liver
-
 Gallus gallus
-
1.1.1.103 liver
-
 Rattus norvegicus
-
1.1.1.103 liver
-
 Sus scrofa
-
1.1.1.103 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.103 3.28
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
 Gallus gallus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Staphylococcus aureus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Rattus norvegicus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Sus scrofa (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Oryctolagus cuniculus (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.103 25 35 85% inactivation after 2 min at 35°C, 10% inactivation at 35°C in the presence of 80 mM KCl Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 8.4 8.5
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+
-
 Gallus gallus
1.1.1.103 NAD+
-
 Staphylococcus aureus
1.1.1.103 NAD+
-
 Rattus norvegicus
1.1.1.103 NAD+
-
 Sus scrofa
1.1.1.103 NAD+
-
 Oryctolagus cuniculus