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Literature summary extracted from

  • Craig, P.A.; Dekker, E.E.
    L-Threonine dehydrogenase from Escherichia coli K-12: thiol-dependent activation by Mn2+ (1986), Biochemistry, 25, 1870-1876.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 Ag+ 0.064 mM, activity is completely blocked Escherichia coli K-12
1.1.1.103 Be2+ 3.2 mM, 20-50% inhibition Escherichia coli K-12
1.1.1.103 Cu2+ 3.2 mM, 20-50% inhibition Escherichia coli K-12
1.1.1.103 Hg2+ 0.064 mM, activity is completely blocked Escherichia coli K-12
1.1.1.103 Ni2+ 3.2 mM, 20-50% inhibition Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.11
-
 NAD+ demetallized enzyme Escherichia coli K-12
1.1.1.103 0.6
-
 NAD+ enzyme saturated with 0.25 mM Mn2+ Escherichia coli K-12
1.1.1.103 1.1
-
 L-threonine demetallized enzyme Escherichia coli K-12
1.1.1.103 221
-
 L-threonine enzyme saturated with 0.25 mM Mn2+ Escherichia coli K-12

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.103 Cd2+ 0.064 or 3.2 mM, 6fold increase of L-threonine dehydrogenase activity Escherichia coli K-12
1.1.1.103 Mn2+ 0.064 or 3.2 mM, 4fold increase of L-threonine dehydrogenase activity, activation is dependent on the presence of a reduced thiol in all enzyme stock solutions and assay buffers, binding of 0.86 mol of Mn2+ per mol of enzyme subunit Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.103 L-threonine + NAD+ Escherichia coli K-12
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Escherichia coli K-12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103
-
 Escherichia coli K-12

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.103 8
-
 fully demetalized enzyme Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
 Escherichia coli K-12 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+
-
 Escherichia coli K-12