BRENDA - Enzyme Database

Properties of the enzymes catalyzing the biosynthesis of lysophosphatidate and its ether analog in cultured fibroblasts from Zellweger syndrome patients and normal controls

Webber, K.O.; Datta, N.S.; Hajra, A.K.; Arch. Biochem. Biophys. 254, 611-620 (1987)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.0051
-
NADPH
skin fibroblasts, normal cells
Homo sapiens
1.1.1.101
0.0054
-
NADPH
skin Zellwenger syndrome fibroblasts
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Homo sapiens
acyldihydroxyacetone phosphate pathway
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Homo sapiens
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Homo sapiens
acyldihydroxyacetone phosphate pathway
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.101
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
skin fibroblast
Zellweger syndrome cell lines GM0228 and GM4340 and normal control cells
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285689
Homo sapiens
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
acyldihydroxyacetone phosphate pathway
285689
Homo sapiens
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285689
Homo sapiens
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
acyldihydroxyacetone phosphate pathway
285689
Homo sapiens
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285689
Homo sapiens
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.101
50
-
t1/2: 15 min, control fibroblasts, t1/2: 20 min, Zellweger syndrome fibroblasts
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.0051
-
NADPH
skin fibroblasts, normal cells
Homo sapiens
1.1.1.101
0.0054
-
NADPH
skin Zellwenger syndrome fibroblasts
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Homo sapiens
acyldihydroxyacetone phosphate pathway
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Homo sapiens
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Homo sapiens
acyldihydroxyacetone phosphate pathway
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Homo sapiens
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
skin fibroblast
Zellweger syndrome cell lines GM0228 and GM4340 and normal control cells
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285689
Homo sapiens
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
acyldihydroxyacetone phosphate pathway
285689
Homo sapiens
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285689
Homo sapiens
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
acyldihydroxyacetone phosphate pathway
285689
Homo sapiens
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285689
Homo sapiens
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285689
Homo sapiens
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.101
50
-
t1/2: 15 min, control fibroblasts, t1/2: 20 min, Zellweger syndrome fibroblasts
Homo sapiens