BRENDA - Enzyme Database

Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers

Ghosh, M.K.; Hajra, A.K.; Arch. Biochem. Biophys. 245, 523-530 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.101
Clofibrate
activation
Mus musculus
General Stability
EC Number
General Stability
Organism
1.1.1.101
NADPH enhances thermal stability
Cavia porcellus
1.1.1.101
stable toward high concentrations of N-ethylmaleimide or DTNB
Cavia porcellus
1.1.1.101
thiols stabilize solubilized enzyme
Cavia porcellus
1.1.1.101
Triton X-100, stable to solubilization in the presence of high salt concentrations
Cavia porcellus
1.1.1.101
trypsin inactivates
Cavia porcellus
1.1.1.101
trypsin inactivates
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.101
alkyldihydroxyacetone phosphate
at high concentrations
Cavia porcellus
1.1.1.101
additional information
DTNB and thiols; stable to N-ethylmaleimide
Cavia porcellus
1.1.1.101
NADP+
competitive to NADPH
Cavia porcellus
1.1.1.101
palmitoyl-CoA
-
Cavia porcellus
1.1.1.101
Trypsin
inactivation
Cavia porcellus
1.1.1.101
Trypsin
inactivation
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.01
-
NADPH
Km-value below: hexadecyldihydroxyacetone phosphate + NADPH, microsomal enzyme
Cavia porcellus
1.1.1.101
0.01
-
NADPH
-
Rattus norvegicus
1.1.1.101
0.02
-
NADPH
,cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme; cosubstrate palmitoyldihydroxyacetone phosphate, microsomal enzyme
Cavia porcellus
1.1.1.101
0.05
-
NADPH
hexadecyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.06
-
NADPH
cosubstrate palmitoyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.07
-
NADPH
in presence of NADP+, microsomal enzyme
Cavia porcellus
1.1.1.101
0.08
-
NADH
cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme, NADPH, cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.13
-
NADPH
in presence of NADP+, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.8
-
NADH
cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Mus musculus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Mus musculus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Rattus norvegicus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Mus musculus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Mus musculus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Rattus norvegicus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.101
Cavia porcellus
-
-
-
1.1.1.101
Mus musculus
-
-
-
1.1.1.101
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.101
partial, solubilization
Cavia porcellus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
liver
-
Mus musculus
-
1.1.1.101
liver
-
Rattus norvegicus
-
1.1.1.101
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.101
0.0186
-
light mitochondrial fraction
Mus musculus
1.1.1.101
0.0296
-
light mitochondrial fraction, clofibrate-fed animals
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285688
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285688
Mus musculus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285688
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285688
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285688
Mus musculus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285688
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285688
Mus musculus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
ether analog of acyl dihydroxyacetone phosphate
285688
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
ether analog of acyl dihydroxyacetone phosphate
285688
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285688
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285688
Mus musculus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285688
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285688
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285688
Rattus norvegicus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285688
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285688
Rattus norvegicus
1-palmitoylglycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.101
55
-
10% loss of activity after 15 min, NADPH enhances thermal stability
Cavia porcellus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.101
6.5
-
-
Cavia porcellus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.101
5.5
8.2
about half-maximal activity at pH 5.5 and 8.2
Cavia porcellus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADH
-
Cavia porcellus
1.1.1.101
NADH
-
Rattus norvegicus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Rattus norvegicus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.101
Clofibrate
activation
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.101
NADH
-
Cavia porcellus
1.1.1.101
NADH
-
Rattus norvegicus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Rattus norvegicus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Cavia porcellus
1.1.1.101
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.101
NADPH enhances thermal stability
Cavia porcellus
1.1.1.101
stable toward high concentrations of N-ethylmaleimide or DTNB
Cavia porcellus
1.1.1.101
thiols stabilize solubilized enzyme
Cavia porcellus
1.1.1.101
Triton X-100, stable to solubilization in the presence of high salt concentrations
Cavia porcellus
1.1.1.101
trypsin inactivates
Cavia porcellus
1.1.1.101
trypsin inactivates
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.101
alkyldihydroxyacetone phosphate
at high concentrations
Cavia porcellus
1.1.1.101
additional information
DTNB and thiols; stable to N-ethylmaleimide
Cavia porcellus
1.1.1.101
NADP+
competitive to NADPH
Cavia porcellus
1.1.1.101
palmitoyl-CoA
-
Cavia porcellus
1.1.1.101
Trypsin
inactivation
Cavia porcellus
1.1.1.101
Trypsin
inactivation
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.101
0.01
-
NADPH
Km-value below: hexadecyldihydroxyacetone phosphate + NADPH, microsomal enzyme
Cavia porcellus
1.1.1.101
0.01
-
NADPH
-
Rattus norvegicus
1.1.1.101
0.02
-
NADPH
,cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme; cosubstrate palmitoyldihydroxyacetone phosphate, microsomal enzyme
Cavia porcellus
1.1.1.101
0.05
-
NADPH
hexadecyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.06
-
NADPH
cosubstrate palmitoyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.07
-
NADPH
in presence of NADP+, microsomal enzyme
Cavia porcellus
1.1.1.101
0.08
-
NADH
cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme, NADPH, cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.13
-
NADPH
in presence of NADP+, peroxisomal enzyme
Cavia porcellus
1.1.1.101
0.8
-
NADH
cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Mus musculus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Mus musculus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
Rattus norvegicus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Mus musculus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Mus musculus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
Rattus norvegicus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.101
partial, solubilization
Cavia porcellus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.101
liver
-
Mus musculus
-
1.1.1.101
liver
-
Rattus norvegicus
-
1.1.1.101
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.101
0.0186
-
light mitochondrial fraction
Mus musculus
1.1.1.101
0.0296
-
light mitochondrial fraction, clofibrate-fed animals
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285688
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285688
Mus musculus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
-
285688
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285688
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285688
Mus musculus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285688
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
-
285688
Mus musculus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
ether analog of acyl dihydroxyacetone phosphate
285688
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
ether analog of acyl dihydroxyacetone phosphate
285688
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285688
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285688
Mus musculus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Mus musculus
?
1.1.1.101
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285688
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285688
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
hexadecyldihydroxyacetone phosphate + NADPH
-
285688
Rattus norvegicus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285688
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285688
Cavia porcellus
?
1.1.1.101
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285688
Rattus norvegicus
1-palmitoylglycerol 3-phosphate + NADP+
-
285688
Rattus norvegicus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.101
55
-
10% loss of activity after 15 min, NADPH enhances thermal stability
Cavia porcellus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.101
6.5
-
-
Cavia porcellus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.101
5.5
8.2
about half-maximal activity at pH 5.5 and 8.2
Cavia porcellus