Any feedback?
Literature summary extracted from
- Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers (1986), Arch. Biochem. Biophys., 245, 523-530.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.101 | Clofibrate | activation | Mus musculus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.101 | NADPH enhances thermal stability | Cavia porcellus |
| 1.1.1.101 | stable toward high concentrations of N-ethylmaleimide or DTNB | Cavia porcellus |
| 1.1.1.101 | thiols stabilize solubilized enzyme | Cavia porcellus |
| 1.1.1.101 | Triton X-100, stable to solubilization in the presence of high salt concentrations | Cavia porcellus |
| 1.1.1.101 | trypsin inactivates | Cavia porcellus |
| 1.1.1.101 | trypsin inactivates | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.101 | alkyldihydroxyacetone phosphate | at high concentrations | Cavia porcellus | |
| 1.1.1.101 | additional information | DTNB and thiols; stable to N-ethylmaleimide | Cavia porcellus | |
| 1.1.1.101 | NADP+ | competitive to NADPH | Cavia porcellus | |
| 1.1.1.101 | palmitoyl-CoA | - |
Cavia porcellus | |
| 1.1.1.101 | Trypsin | inactivation | Cavia porcellus | |
| 1.1.1.101 | Trypsin | inactivation | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.101 | 0.01 | - |
NADPH | - |
Rattus norvegicus | |
| 1.1.1.101 | 0.01 | - |
NADPH | Km-value below: hexadecyldihydroxyacetone phosphate + NADPH, microsomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.02 | - |
NADPH | cosubstrate palmitoyldihydroxyacetone phosphate, microsomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.02 | - |
NADPH | ,cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.05 | - |
NADPH | hexadecyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.06 | - |
NADPH | cosubstrate palmitoyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.07 | - |
NADPH | in presence of NADP+, microsomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.08 | - |
NADH | cosubstrate hexadecyldihydroxyacetone phosphate, microsomal enzyme, NADPH, cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.08 | - |
NADH | enzyme isolated from microsome | Cavia porcellus | |
| 1.1.1.101 | 0.13 | - |
NADPH | in presence of NADP+, peroxisomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.8 | - |
NADH | cosubstrate hexadecyldihydroxyacetone phosphate, peroxisomal enzyme | Cavia porcellus | |
| 1.1.1.101 | 0.8 | - |
NADH | enzyme isolated from peroxisome | Cavia porcellus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | Cavia porcellus | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | Mus musculus | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | Rattus norvegicus | reaction in non-ether lipids biosynthesis | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | Cavia porcellus | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | Mus musculus | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | Rattus norvegicus | reaction in ether lipids biosynthesis | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.101 | Cavia porcellus | - |
- |
- |
| 1.1.1.101 | Mus musculus | - |
- |
- |
| 1.1.1.101 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.101 | partial, solubilization | Cavia porcellus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.101 | liver | - |
Cavia porcellus | - |
| 1.1.1.101 | liver | - |
Mus musculus | - |
| 1.1.1.101 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.101 | 0.0186 | - |
light mitochondrial fraction | Mus musculus |
| 1.1.1.101 | 0.0296 | - |
light mitochondrial fraction, clofibrate-fed animals | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | - |
Mus musculus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | - |
Rattus norvegicus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Cavia porcellus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Mus musculus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | acyldihydroxyacetone phosphate + NADPH | reaction in non-ether lipids biosynthesis | Rattus norvegicus | 1-acyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | - |
Mus musculus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | ether analog of acyl dihydroxyacetone phosphate | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | ether analog of acyl dihydroxyacetone phosphate | Rattus norvegicus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Cavia porcellus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Mus musculus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | alkyldihydroxyacetone phosphate + NADPH + H+ | reaction in ether lipids biosynthesis | Rattus norvegicus | 1-alkyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | hexadecyl dihydroxyacetone phosphate + NADH + H+ | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NAD+ | - |
r | |
| 1.1.1.101 | hexadecyldihydroxyacetone phosphate + NADPH | - |
Cavia porcellus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | hexadecyldihydroxyacetone phosphate + NADPH | - |
Rattus norvegicus | 1-hexadecyl-sn-glycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Cavia porcellus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? | |
| 1.1.1.101 | palmitoylglycerone phosphate + NADPH | i.e. palmitoyldihydroxyacetone phosphate | Rattus norvegicus | 1-palmitoylglycerol 3-phosphate + NADP+ | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.101 | 55 | - |
10% loss of activity after 15 min, NADPH enhances thermal stability | Cavia porcellus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.101 | 6.5 | - |
- |
Cavia porcellus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.101 | 5.5 | 8.2 | about half-maximal activity at pH 5.5 and 8.2 | Cavia porcellus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.101 | NADH | - |
Cavia porcellus | |
| 1.1.1.101 | NADH | - |
Rattus norvegicus | |
| 1.1.1.101 | NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Cavia porcellus | |
| 1.1.1.101 | NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Mus musculus | |
| 1.1.1.101 | NADPH | hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates | Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
